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Quantitative screening method for embrittling special rice

A screening method and technology for special rice, applied in the field of agricultural science, can solve the problems of brittleness screening and quantitative statistics of brittleness degree, etc., and achieve the effect of saving labor and cost

Inactive Publication Date: 2015-10-14
YIELEAD RICE IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Although the traits of brittle-stem rice are easy to qualitatively distinguish from other conventional rice varieties, as long as the stems or leaves are broken by hand, it can be judged intuitively, but the degree of brittleness cannot be quantitatively counted, which gives the genetic location of the multigenic brittle mutants. The fragility selection in the process of polygenic aggregation breeding of fragility genes has brought troubles

Method used

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  • Quantitative screening method for embrittling special rice
  • Quantitative screening method for embrittling special rice
  • Quantitative screening method for embrittling special rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1. Comparison of puncture test and creasing test between brittle rice at heading stage and conventional rice with different treatments

[0032] Freshly refrigerated brittle rice at the heading stage and conventional rice Zhendao 88 and Lianjing 7 were taken respectively, and the non-leaf-stripping puncture test, leaf-stripping puncture test, puncture test after dewaxing and embrittlement treatment (this invention) and destruction test were carried out respectively. , using the measurement data of the plant lodging resistance instrument YYD-1A of Zhejiang Top Instrument Company, the unit is: kPa or N.

[0033] Puncture test: take freshly refrigerated brittle-stem mutant materials at the heading stage and conventional rice varieties Zhendao 88 and Lianjing 7, and puncture at a place 1.5-2 cm above the beginning of the first internode near the root, record the data, and select Different tillers were punctured, repeated 10 times; in addition, the rice leaves wrappe...

Embodiment 2

[0040] Example 2. The comparison between the brittle stem material at the heading stage of the dewaxing embrittlement treatment and the puncture at different positions of the conventional rice

[0041] The brittle stem materials at the heading stage and the conventional rice varieties Zhendao 88 and Lianjing 7 were respectively taken, and after dewaxing and embrittlement treatment, the flag leaf puncture test and the first internode (the present invention), the second internode, In the third internode puncture test, the plant lodging resistance instrument YYD-1A of Zhejiang Top Instrument Company was used to measure the data, the unit is kPa, and the results are shown in Table 2.

[0042]

[0043] The result finds, adopts flag leaf puncture, brittle stem material / zhendao 88=6.8 / 9.0=0.76, brittle stem / Lianjing No. 7=6.8 / 9.3=0.74; Stem / Zhendao 88=0.45, fragile stem / Lianjing No. 7=0.43, so adopting the technology of the present invention is more conducive to the quantitative...

Embodiment 3

[0045] Example 3. Comparison of brittle stem materials in different growth stages and puncture of conventional rice varieties

[0046] Freshly refrigerated brittle stem materials at the full tillering stage, heading stage, and mature stage were taken respectively, and conventional rice varieties Zhendao 88 and Lianjing 7 were dewaxed and embrittled to conduct the first internode leaf-stripping stem puncture test. The lodging resistance of the plants was Instrument YYD-1A measurement data, the unit is: kPa, the result table 3.

[0047]

[0048] The puncture comparison experiments between brittle stem materials at different growth stages and conventional rice varieties with stripped leaves and stalks found that the puncture comparisons between brittle stem materials at the peak tillering stage and conventional rice varieties were 25.7 / 50.8=0.51 and 25.7 / 54.7=0.47; The puncture ratios of brittle stem materials at early stage and conventional varieties were 0.45 and 0.43 resp...

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Abstract

The invention provides a quantitative screening method for embrittling special rice. According to the quantitative screening method, a specific embrittlement treatment method and a detection method are adopted to to-be-screened materials for detection, and accurate numerical indices are obtained by identifying detection organs and parts with the notable difference degree and detection periods among the to-be-screened materials, ultimately realizing notable and accurate quantitative screening of the phenotypic difference degree among embrittling special rice. The problems of embrittling stalk gene mapping of embrittling special rice breeding and inaccuracy in embrittlement phenotypic difference degree screening in multiple genes pyramiding breeding are solved, thereby greatly saving manpower and cost of molecular detection, and realizing accurate quantitative screening of the embrittling special rice.

Description

technical field [0001] The invention relates to the field of agricultural science and technology, in particular to a method capable of quantitatively evaluating and screening brittle special rice through numerical indexes. Background technique [0002] The so-called brittle special rice refers to the special rice cultivated by using the rice brittle stem mutant, which inherits the heritable variation of the brittle stem and other tissues and organs of the brittle stem mutant, and finally shows the rice cell wall brittleness Altered trait variation. The brittle stem mutation refers to the mutation that increases the brittleness of the plant stem. It is a relatively common type of mutation in plants. Usually, the roots, stems, leaves, stem nodes and other organs show a phenotype of increased brittleness and fragility. Arabidopsis, maize, etc. , tomato, rice and other plants have been reported. [0003] In 1963, Nagao and Takahashi first discovered the rice brittle stem trait...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00
CPCA01G7/00
Inventor 不公告发明人
Owner YIELEAD RICE IND
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