Application of protein m57 in regulating rice resistance to ammonium

A protein and rice technology, applied in the biological field, can solve problems such as damage, affecting the growth and yield of rice, and the effective use of nitrogen fertilizers

Active Publication Date: 2022-05-17
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The yield of rice is very dependent on the application of nitrogen fertilizer, but the application of nitrogen fertilizer also has many serious negative effects. For example, most of the applied nitrogen fertilizer cannot be effectively used by rice, but seeps into groundwater and volatilizes into groundwater in the form of various forms of nitrogen compounds. In the air, the nitrogen cycle causes pollution and destruction of large areas of water resources, soil and ecosystems
In addition, rice is a crop that likes ammonium nitrogen fertilizers, but excessive ammonium fertilizers are toxic to rice, affecting the growth and yield of rice.

Method used

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  • Application of protein m57 in regulating rice resistance to ammonium
  • Application of protein m57 in regulating rice resistance to ammonium
  • Application of protein m57 in regulating rice resistance to ammonium

Examples

Experimental program
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Embodiment 1

[0059] Example 1, T 3 Obtainment of homozygous transgenic OsMADS57-R rice and identification of its resistance to ammonium and agronomic traits

[0060] 1. Construction of recombinant plasmid pGEM-T-MADS57

[0061] 1. Using 10-day-old seedlings of rice variety Nipponbare (hereinafter referred to as Nipponbare) as experimental materials, total RNA was extracted with Trizol reagent to obtain Nipponbare RNA. Take Nipponbare RNA, first use DNA gel to detect the quality of RNA, and then use a nanodrop spectrophotometer to measure the concentration of RNA.

[0062] 2. After completing step 1, take 1 μL of Nipponbare RNA and perform reverse transcription with a plant cDNA synthesis kit to obtain 20 μL of cDNA product (the cDNA product is the cDNA of Nipponbare).

[0063] 3. After completing step 2, use the cDNA product as a template, MADS57-Fp: 5′-atggggagggggaagatagt-3′ and MADS57-Rp: 5′-ttaaggcagatgaagtccca-3′ as primers, and use high-fidelity Phusion DNA polymerase for PCR ampli...

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Abstract

The invention discloses the application of protein M57 in regulating the resistance of rice to ammonium. A recombinant vector expressing protein M57 was introduced into Nipponbare to obtain T 0 Transgenic rice with OsMADS57‑R gene; T 0 Transgenic OsMADS57‑R gene rice was continuously selfed for three generations to obtain T 3 Generation of homozygous transgenic OsMADS57‑R rice. Compared with Nippon Clear, T 3 The generation homozygous OsMADS57‑R transgenic rice had stronger growth in the vegetative growth period, significantly increased leaf width, significantly thicker stems, more compact plant shape, significantly increased leaf angle, and significantly decreased plant height at the mature stage. Grain weight, grain width and yield increased significantly after harvesting, as did panicle length and grain number per panicle. It can be seen that protein M57 plays an important role in regulating rice resistance to ammonium, yield, plant height, grain weight, grain width, panicle length, grain number per panicle, leaf width, stem diameter and leaf angle. The invention has important application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of protein M57 in regulating the resistance of rice to ammonium. Background technique [0002] Rice is one of the most important food crops in the world. More than 120 countries around the world grow rice, and the cultivated area is maintained at more than 150 million hectares all year round. 50% of the world's population uses rice as a staple food. The yield of rice is very dependent on the application of nitrogen fertilizer, but the application of nitrogen fertilizer also has many serious negative effects. For example, most of the applied nitrogen fertilizer cannot be effectively used by rice, but seeps into groundwater and volatilizes into groundwater in the form of various forms of nitrogen compounds. In the air, the nitrogen cycle causes pollution and damage to large areas of water resources, soil and ecosystems. In addition, rice is a crop that prefe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H5/12A01H6/46
CPCC07K14/415C12N15/8261C12N15/8271
Inventor 颜永胜方荣祥陈晓英焦晓明
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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