Wheat stripe rust resistance genes and methods of use

A gene and resistance technology, applied in the field of disease resistance genes to enhance plant resistance to plant diseases, can solve the problems of destroying the linkage of harmful alleles, long breeding timeline, etc.

Active Publication Date: 2018-11-06
TWO BLADES FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional methods for the introduction of R genes often involve long breeding timelines that disrupt linkages with deleterious alleles of other genes

Method used

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  • Wheat stripe rust resistance genes and methods of use
  • Wheat stripe rust resistance genes and methods of use
  • Wheat stripe rust resistance genes and methods of use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0233] Example 1: Isolation and Fine Mapping of Rps6

[0234]Mapping of Rps6: Different phenotypes were observed between Abed Binder 12 and Russell inoculated with Pst isolates 08 / 501 and 08 / 21. Russell showed less pustules but had a clear microscopic phenotype of colonization ( figure 1 ). We used Pst isolate 08 / 501 (AxR-Pst) to inoculate Abed Binder 12 x Russell F 2 group. Parental, F 1 and 92 F 2 Plants were phenotyped. For AxR-Pst F 2 Pustule formation was not observed for the population, but segregation was observed for chlorosis and pCOL ( figure 2 A-2B). A strong correlation between chlorosis and pCOL was observed (r 2 =0.88)( figure 2 C). f 1 Shows a resistance phenotype similar to Abed Binder 12. To map resistance to Pst, we used the barley oligonucleotide assay for AxR-Pst F 2 The population was phenotyped (BOPA1; 1,536 SNP-based markers; Close et al. 2009). Between Abed Binder 12 and Russell, a total of 535 polymorphic OPA markers were identified an...

Embodiment 2

[0308] Example 2: Transformation of barley using Rps6 and testing for wheat stripe rust resistance

[0309] Yeo et al. ((2014) Theor.Appl.Genet.127:325–337) previously established the double haploid population SusPtrit x Golden Promise (SxGP DH) to identify transformable barley numbers that have a significant contribution to barley A variety of heterologous rust pathogens are also susceptible. We found that accession SxGP DH-47 is susceptible to wheat stripe rust and was previously shown to be competent for Agrobacterium-based transformation (Yeo et al. (2014) Theor. Appl. Genet. 127:325-337). Transformation of SxGP DH-47 barley was performed as described by Bartlett et al. ((2008) Plant Methods 4:22) with the modification that immature embryogenic calli were infected with Agrobacterium tumefaciens instead of immature Embryo. Briefly, immature embryos, approximately 1.5 to 2 mm in diameter, were harvested from barley plants grown in the greenhouse. In contrast to what was des...

Embodiment 3

[0311] Example 3: Transformation of wheat by Rps6 and test for resistance to wheat stripe rust

[0312] Transformation of wheat (Tyticum vulgaris "Fielder") was performed as described by Periyannan et al. ((2013) Science 341 :786-788). The T-DNA insert comprises the original promoter and coding sequence for NLR-A (SEQ ID NO: 17) from barley contained in a T-DNA plasmid (SEQ ID NO: 15, 34 or 35), As described in Example 2, and a genomic fragment of the open reading frame of NLR-A (SEQ ID NO: 2) fused to the promoter and terminator of a highly expressed NLR gene from wheat. Phenotypic screening of transformed wheat plants for resistance or susceptibility to wheat stripe rust was performed against barley as described above in Example 2.

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Abstract

Compositions and methods for enhancing the resistance of wheat and barley plants to wheat stripe rust caused by Puccinia striiformis f. sp. tritici are provided. The compositions comprise nucleic acidmolecules encoding resistance (R) gene products and variants thereof and plants, seeds, and plant cells comprising such nucleic acid molecules. The methods for enhancing the resistance of wheat and barley plants to wheat stripe rust comprise introducing a nucleic acid molecule encoding an R gene product into a wheat or barley plant cell. Additionally provided are methods for using the wheat and barley plants in agriculture to limit wheat stripe rust.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of U.S. Provisional Patent Application No. 62 / 250,136, filed November 3, 2015, the contents of which are incorporated herein by reference in their entirety. [0003] About Sequence Listings Submitted as Text Files [0004] The official text of the Sequence Listing is submitted electronically via EFS-Web as a Sequence Listing in ASCII format, with file name 070294-0106SEQLST.TXT, created on November 1, 2016, and is 440 kilobytes in size, and is identical to the specification Commit at the same time. The Sequence Listing contained in this document in ASCII format is part of the specification and is hereby incorporated by reference in its entirety. technical field [0005] The present invention relates to the fields of gene separation and plant improvement, in particular to enhancing the resistance of plants to plant diseases by using disease resistance genes. Background technique ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H5/10A01H6/46C07K14/415C12N15/82
CPCA01H5/10C07K14/415C12N15/8282A01H6/4624A01H6/4678Y02A40/146
Inventor M·J·莫斯库A·M·道森
Owner TWO BLADES FOUND
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