The estrogenic gene ferr and the estrogenic gene ferr-r in Populus americana and their application

A gene and black poplar technology, applied in the field of plant genetic engineering, can solve the problem that sex-determining genes have not been cloned and determined.

Active Publication Date: 2021-05-25
NANJING FORESTRY UNIV
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  • Description
  • Claims
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Problems solved by technology

But the sex-determining gene of poplar has not been cloned and determined

Method used

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  • The estrogenic gene ferr and the estrogenic gene ferr-r in Populus americana and their application
  • The estrogenic gene ferr and the estrogenic gene ferr-r in Populus americana and their application
  • The estrogenic gene ferr and the estrogenic gene ferr-r in Populus americana and their application

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Embodiment 1

[0042] (1) Populus nigra full-sib population construction and preliminary sex-determining gene mapping

[0043] In 2012, we constructed a full-sib population of Populus americana consisting of 1077 offspring. The population was planted in Sihong Forest Farm, Jiangsu Province with a row spacing of 6×6 meters, and the population had reached sexual maturity. A genetic map covering the whole genome was constructed for this population, some individuals were randomly selected from the mapping population, and a genome-wide scan of the sex-determining genes of poplar was performed. Sex-determining genes are located at the end of chromosome 19, between the telomere and the N110 marker ( figure 1 ). Sex linkage analysis also found that the sex-determining genes of poplar were heterozygous for males, which indicated that the sex determination system of Populus nigra was XY.

[0044] (2) Fine mapping of sex-determining genes and construction of chromosomal haplotypes in sex-determining ...

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Abstract

The invention discloses an estrogen-promoting gene FERR and an estrogen-repressing gene FERR-R of Populus americana and applications thereof, belonging to the technical field of plant genetic engineering. The present invention fine-positions the sex-determining gene loci of Populus americana through family linkage analysis, and then uses natural population materials to carry out genome-wide association analysis, combined with transcriptome and genome methylation sequencing, and discloses for the first time the poplar estrogen-promoting genes FERR and inhibitory genes. The female gene FERR-R, the FERR-R gene does not encode a protein, but produces siRNAs to methylate the promoter sequence of the female-promoting gene FERR and cut its transcript, thereby inhibiting the female-promoting gene FERR in male poplar plants expression. However, the female poplar plant does not contain the FERR-R gene, and the expression of FERR is not inhibited by FERR-R, so the female flower of the female plant develops. The poplar estrogenic gene and estrogenic suppressor gene provided by the invention can be applied to early sex identification and molecular breeding of poplar.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and more specifically relates to an estrogen-promoting gene FERR and an estrogen-repressing gene FERR-R of American black poplar (Poplusdeltoides) and applications thereof. Background technique [0002] The hypothesis of the origin of plant sex chromosomes holds that sex chromosomes originate from a pair of autosomes. The evolution of the sex chromosomes begins with a male or female sterile mutation on one chromosome of the pair of autosomes. The hypothesis of the origin of plant sex chromosomes holds that sex chromosomes originate from a pair of autosomes. The evolution of sex chromosomes begins with a male or female sterile mutation on one chromosome of the pair of autosomes. Due to some unknown reason, the recombination of the mutation site is inhibited, and the non-recombination region gradually expands to form a heterozygous sex chromosome. Dioecious plants are evolutiona...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/02C12Q1/6895
CPCC07K14/415C12N15/827C12Q1/6895
Inventor 尹佟明薛良交吴怀通陈赢男陆静李小平侯静戴晓港
Owner NANJING FORESTRY UNIV
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