43results about How to "Reduce separation and purification process" patented technology

The process of biosynthesizing gamma-amino butyric acid includes the following steps: slant agar culture to activate Lactobacillus brevis with the preservation number of CGMCC No. 1306, inoculating to GYP or MRS seed culture medium to culture for 10-30 hr, inoculating to GYP or MRS fermenting culture medium in the amount of 0.5-5 % to stationarily culture at 25-35 deg.c for 48-120 hr to obtain fermented liquid containing thallus and centrifugally separating and collecting thallus; washing thallus with sterilized deionized water, suspending wet thallus in citric acid-disodium hydrogen phosphate buffering system with sodium L-glutamate to react for 1-10 hr, and centrifuging the reacted liquid to obtain gamma-amino butyric acid solution. The present invention has low production cost, mild reaction condition, less environmental pollution, simple process, high production efficiency, and high product purity.

The invention provides a method for synthesising halogenated aromatic amine through hydrogenation in a solvent-free condition. The method comprises the following step of: carrying out a liquid-phase hydrogenation reaction on the halogenated aromatic nitro compound shown in formula (I) under the action of hydrogen, in the absence of a solvent and a dehalogenation inhibitor under the action of a carbon-supported large-particle-size precious metal catalyst to prepare the halogenated aromatic amine shown in formula (II). The method provided by the invention is capable of achieving the effect of inhibiting a hydrogenation dehalogenation side reaction in the case of not adding a dehalogenation inhibitor, is high in target product selectivity, and is capable of remarkably increasing the reaction speed.

The invention belongs to the field of recycling of solid waste, and provides a method for preparing liquid fuel by using biomass. The method is characterized by comprising the following steps that thereaction phase and the extract phase are mixed to form a two-phase system to serve as a reaction medium; an acid catalyst, a hydrodeoxygenation catalyst, a biomass raw material and the reaction medium are added into a reactor at the specific mass ratio; a hydrogen source is introduced to have the continuous reaction under the specific temperature and stirring conditions, and cellulose and hemicellulose in the biomass are conversed to be 2,5-2-methylfuran and 2-methylfuran respectively; the water phase and the organic phase are separated, the organic phase rich in 2,5-2-methylfuran and 2-methylfuran is obtained to serve as the liquid fuel, and the unreacted rest materials in the water phase are recycled. The method provided by the invention does not need to pre-process the biomass, can lower temperature and hydrogen consumption and loss needed by the reaction, reduces the separation and purification process, and can integrate 2,5-2-methylfuran and methylfuran into the fuel product directly.

The invention discloses a synthesis method of polysubstituted sulfur-containing or selenium-containing benzo[c,d]indole compounds. Firstly, 8-alkynylnaphthylamine compounds, thioether (diselenide), acatalyst and an additive are placed in a reactor, a solvent is added to the reactor until 8-alkynylnaphthylamine compounds and thioether (diselenide) are completely dissolved, then, the mixture is heated to react, after the reaction, aftertreatment is performed, and the polysubstituted sulfur-containing or selenium-containing benzo[c,d]indole compounds are obtained. The polysubstituted sulfur-containing or selenium-containing benzo[c,d]indole compounds are synthesized with a one-pot one-step method, the reaction operation is simple, intermediate separation and purification processes are reduced, the reaction condition is mild, the reaction selectivity is good, the yield is high, the substrate universality is wide, the reaction materials are simple and easy to obtain, the production cost islow, and the method is applicable to large-scale industrial production as well as small-scale laboratory preparation.

The invention belongs to the field of organic solid waste resourceful utilization, and discloses a method for preparing 5-alkoxy methyl furfural from carbohydrate. The method is characterized by including the following steps of establishing a two-phase system as the reaction medium by using ionic liquid with bromide ions or a deep-eutectic solvent with bromide ions as the reaction phase and usingan organic solvent as the extraction phase, adding a solid acid catalyst with strong Lewis acidity, a carbohydrate raw material, an alcohol raw material and the reaction medium to a reactor, and continuously converting the carbohydrate raw material into 5-alkoxy methyl furfural under the heating and stirring conditions. By means of the method, the Bronsted acid catalysis side reaction can be reduced, the reaction efficiency and selectivity are greatly improved, the conversion efficiency, green property and economy of fuel additives for converting the carbohydrate into 5-alkoxy methyl furfuralare improved, the subsequent separation and purification process can be reduced with liquid fuel and the like as the extraction phase, and products are directly integrated in liquid fuel products.

The invention provides a mixed rare-earth modified methane steam reformed nickel-based catalyst and a preparation method thereof. According to the mixed rare-earth modified methane steam reformed nickel-based catalyst, nickel nitrate and the like are used as precursors and are directly immersed and loaded on spherical aluminum oxide modified by mixed rare earth. The mixed rare earth is obtained by mixing two selected from lanthanum-cerium, lanthanum-yttrium, lanthanum-praseodymium, lanthanum-samarium and the like, wherein the loading weight of nickel in the catalyst is 5-30% of the weight of the catalyst and the loading weight of the mixed rare earth is 0.5-10% of the weight of the catalyst; the ratio of the mixed rare earth is 7 / 3-3 / 7. With the adoption of the mixed rare earth, the separation cost of the rare earth can be greatly reduced; the preparation method of the catalyst is simple; the catalyst has the good catalytic activity and stability on reforming methane steam in a fixed bed.

Disclosed is a rice variety resource false smut resistance disease nursery identification method. According to the method, a disease nursery is built on a piece of paddy field which is pathogenetic all year around in a region where false smut is popular; by planting pathogenetic varieties on the disease nursery, scattering false smut balls, increasing field humidity, unevenly applying nitrogen fertilizer and the like, rice is triggered to be pathogenetic, and a good pathogenetic effect can be acquired. Compared with prior artificial inoculation, identification of a large number of variety resources can be carried out at the same time, and the workload is greatly reduced. Complex work like separation, purifying and culture of artificially-inoculated pathogenic bacteria is avoided, and the identification technical difficulty is reduced. False smut resistance of a large number of rice variety resources, new strains, new combinations and breeding materials can be identified at the same time. The method provides evidence for false smut resistance resource selection, breeding for disease resistance, rice variety certification and the like, and the risk of an outbreak of false smut in rice planting is reduced. The method has good market application prospect.

The invention discloses a group of novel 3-cyanoindole compounds listed as formula I; wherein, R1 is equal to H, R2 is equal to 7-Br; or R1 is equal to H, R2 is equal to 7-C1; or R1 is equal to H, R2 is equal to 6-F; or R1 is equal to H, R2 is equal to 7-F; or R1 is equal to H, R2 is equal to 7-OCH3; or R1 is equal to 4-CH3, R2 is equal to 6-CH3; or R1 is equal to 4-CH3, R2 is equal to 7-CH3; or R1 is equal to 6-CH3, R2 is equal to 7-CH3. The invention also discloses a synthesis method for the 3-cyano indole compounds listed as formula I. R1 and R2 in formula I are separately H, F, Cl, Br, I, methyl, ethyl, n propyl, isopropyl, butyl, methoxy and benzyloxy, and the steps are that under a temperature of 65 to 180 DEG C, the 3-formylindole compounds and hydroxylamine salt are mixed to be reacted in an organic solvent. The method provided by the invention is easily operated with a low cost and can not only be applied to small-scale facility in a laboratory, but can also be applied to large-scale industrial production.

The invention provides a synthesis method of a copper-promoted 3-cyano-substituted-indole compound. According to the method, a copper reagent which is cheap and easy to obtain is used as a medium, an indole compound, TMEDA (tetramethylethylenediamine) and acetonitrile are used as raw materials, and the 3-cyano-substituted-indole compound is synthesized. The method has the advantages as follows: the raw materials are cheap and easy to obtain, the TMEDA and the acetonitrile are used as sources of cyano-groups, usage of higher-toxicity cyanide is avoided, special reaction conditions such as ligands, acids, bases, superoxides, microwave radiation and the like are not adopted, operation is simple, conditions are moderate, the yield is higher, and the method has the broad industrial application prospect.

The invention belongs to the technical field of perfume synthesis, and particularly relates to a synthetic method of epsilon-decalactone perfume. The synthetic method comprises the following steps: (1) mixing an alkaline solution with a part of cyclohexanone, then dropwise adding a mixture of n-butyraldehyde and the rest of cyclohexanone into the mixture, carrying out stirring, and carrying out heat preservation for a reaction; (2) standing the reactants in the step (1), collecting a water layer, collecting an oil layer, and washing the oil layer with water to obtain a condensed product 2-butenyl cyclohexanone crude product; (3) carrying out distilling to recover cyclohexanone to obtain 2-butenyl cyclohexanone; (4) carrying out a hydrogenation reaction; (5) collecting a liquid-phase intermediate product 2-butyl cyclohexanone crude product, and carrying out distilling to obtain 2-butyl cyclohexanone; (6) carrying out an oxidation reaction; and (7) carrying out distilling purification toobtain the epsilon-decalactone perfume product. According to the invention, the cyclohexanone is added into the reaction system in different ways, the excess cyclohexanone is used as a reaction solvent through a stirring dripping technology, and the cyclohexanone is reused after being recycled, so that separation and purification processes of the reaction solvent are reduced, and a high reactionyield is ensured.

A synthetic method of 2-phenyl-3-cyano benzofuran compounds is disclosed. The method includes weighing an aldehyde compound, an aryl acetonitrile compound, a catalyst and an alkali, according to a mole ratio of 1:1-2:0.2:4, adding into a reaction container, adding a solvent into the container until the aldehyde compound and the aryl acetonitrile compound are fully dissolved, putting the reaction container into an oil bath having temperature of 100-120 DEG C, reacting under stirring for 18-24 h, cooling to room temperature, adding water the volume of which is same as the volume of the solvent, extracting with dichloromethane for 2-4 times, separating by a silica column and distilling under vacuum to obtain a product that is one of the 2-phenyl-3-cyano benzofuran compounds. The method adopts a one-pot manner to produce the 2-phenyl-3-cyano benzofuran compounds, reduces processes of separation and purification of intermediates, and is simple in operation method, mild in reaction conditions, simple and easily available in raw materials, low in production cost, suitable for small-scale preparation in a laboratory and suitable for large-scale industrial production.

The invention discloses a preparation method of clethodim and an intermediate thereof. The preparation method of the intermediate comprises the step of acidifying a compound A to obtain a compound B.According to the invention, the compound B is obtained by acidifying the compound A, and reacts to obtain a compound D, and the compound D reacts to obtain clethodim, so that the reaction condition are mild, the operation is simple, the preparation process of the compounds A-D does not require separation, water washing, layering, rearrangement and other operations, the clethodim preparation process flow is simplified, the reaction time is shortened, the production efficiency is improved, the post-treatment is simple, water washing, layering and other processes are not needed, separation and purification are facilitated, the wastewater treatment burden is not increased, three wastes are reduced, the production cost is reduced, the environmental protection requirement is met, and the methodis suitable for industrial production.

The invention discloses a 3-cyanoindole compound synthesis method which includes: firstly, taking indole compound, benzyl cyanide and CuI according to the molar ratio of 1:1-2:1-2, placing the indole compound, the benzyl cyanide and the CuI in a reaction vessel, and adding solvent into the reaction vessel until the indole compound and the benzyl cyanide are completely dissolved; then, placing the reaction vessel in oil bath at the temperature of 100-130 DEG C with stirring for reaction for 30-34h, cooling to the room temperature, adding water as same as the solvent in volume, and extracting for 2-4 times by the aid of dichloromethane; and finally, separating by means of silica gel column chromatography prior to reduced pressure distillation so that the product, namely, the 3-cyanoindole compound is obtained. The 3-cyanoindole compound is synthesized by the aid of 'one-pot' reaction, and accordingly the intermediate separation and purification process is omitted, and the method is simple to operate, mild in reaction conditions, available in reaction raw materials, low in production cost and applicable to small-scale laboratorial preparation and large-scale industrial production.

The present invention discloses new 1,3-dihydroxy-3,7-dimethyl-6-octen-2-one synthesis method, which comprises racemic synthesis and enantioselective synthesis. According to the present invention, commercially available 6-methyl-5-hepten-2-one (CAS:110-93-0) is adopted as a starting material, two one-pot series connection reaction systems are adopted, simple operations are performed, the separation purification of the intermediate is effectively avoided, the high-yield racemic synthesis of the 1,3-dihydroxy-3,7-dimethyl-6-octen-2-one is achieved, and the asymmetric synthesis method for enantioselective synthesis of (R)-1,3-dihydroxy-3,7-dimethyl-6-octen-2-one and (S)-1,3-dihydroxy-3,7-dimethyl-6-octen-2-one is developed. The present invention further discloses an intermediate compound.

A synthetic method of 2-phenyl-3-cyano benzofuran compounds is disclosed. The method includes weighing an aldehyde compound, an aryl acetonitrile compound, a catalyst and an alkali, according to a mole ratio of 1:1-2:0.2:4, adding into a reaction container, adding a solvent into the container until the aldehyde compound and the aryl acetonitrile compound are fully dissolved, putting the reaction container into an oil bath having temperature of 100-120 DEG C, reacting under stirring for 18-24 h, cooling to room temperature, adding water the volume of which is same as the volume of the solvent, extracting with dichloromethane for 2-4 times, separating by a silica column and distilling under vacuum to obtain a product that is one of the 2-phenyl-3-cyano benzofuran compounds. The method adopts a one-pot manner to produce the 2-phenyl-3-cyano benzofuran compounds, reduces processes of separation and purification of intermediates, and is simple in operation method, mild in reaction conditions, simple and easily available in raw materials, low in production cost, suitable for small-scale preparation in a laboratory and suitable for large-scale industrial production.

The invention discloses a transforming adsorbing method to separate and recycle C3+ hydrocarbon from composite gas, which is characterized by the following: the composite gas is filled in the adsorbing tower with adsorbant, which is separated through transforming adsorbing course; the adsorbing tower possesses alumina, activated charcoal and silica gel with middle and micro-hole structure and specific area at 300-1500m2 / g, whose bulk rate is 5-50% alumina, 5-90% activated charcoal, 0-90% silica gel; the content of activated charcoal is more than alumina, which can separate and recycle C3+ hydrocarbon through extracting step.

The invention provides a method for simultaneously preparing guaijaverin and avicularin from guava leaf and use of the method. The method provided by the invention comprises the following steps: crushing guava leaf as the raw material, extracting by using 5-10 times of 30-90% lower alcohol in volume for 2-3 times, each time for 2-3 h, mixing an extracting solution, concentrating in vacuum, and recycling a solvent to obtain an extract; dissolving the extract by adding water, sufficiently extracting by using ethyl acetate, mixing ethyl acetate extraction liquid, and concentrating in vacuum to obtain an ethyl acetate extract; carrying out silica gel column chromatography separation by using the ethyl acetate extract, carrying out gradient elute by using eluents, namely petroleum ether and ethyl acetate, concentrating eluents in vacuum with the volume ratio of petroleum ether to ethyl acetate of 60:40 or 40:60; and adding the concentrated solution into lower alcohol, concentrating and crystallizing in vacuum, filtering, and drying to obtain guaijaverin and avicularin. The method disclosed by the invention is simple and convenient; and guaijaverin and avicularin disclosed by the invention are capable of obviously reducing hyperglycemia induced by streptozotocin and can be used for preparing medicines or health-care products for reducing blood glucose.

The process of biosynthesizing gamma-amino butyric acid includes the following steps: slant agar culture to activate Lactobacillus brevis with the preservation number of CGMCC No. 1306, inoculating to GYP or MRS seed culture medium to culture for 10-30 hr, inoculating to GYP or MRS fermenting culture medium in the amount of 0.5-5 % to stationarily culture at 25-35 deg.c for 48-120 hr to obtain fermented liquid containing thallus and centrifugally separating and collecting thallus; washing thallus with sterilized deionized water, suspending wet thallus in citric acid-disodium hydrogen phosphate buffering system with sodium L-glutamate to react for 1-10 hr, and centrifuging the reacted liquid to obtain gamma-amino butyric acid solution. The present invention has low production cost, mild reaction condition, less environmental pollution, simple process, high production efficiency, and high product purity.

The invention discloses a synthesis method of 7-chloroquinaldine. 2-nitrotoluene, SiO2-HEPIMBr, m-chloroaniline, crotonaldehyde, TEOS, 2-bromoethanol, imidazole and 3-chloropropyltriethoxysilane are taken as main raw materials. According to the synthesis process, m-chloroaniline and crotonaldehyde are subjected to Skraup reaction under action of an immobilized ion catalyst SiO2-HEPIMBr, and 7-chloroquinaldine is obtained. SiO2 immobilized hydroxyl ionic liquid HEPIMBr is prepared with a grafting method, in the reaction, few by-products are produced and no 5-isomer is produced, so that the separation and purification process is reduced, purity of a product is improved, yield of the product is increased, and higher market competitiveness is achieved.

The invention discloses a synthesis method of a polysubstituted benzo[c, d] indole compound. The synthesis method comprises the following steps: taking an 8-alkynyl naphthylamine compound, a catalystand alkali according to a molar ratio of 1:(0.1-0.5):(1-2), and putting into a reaction container; adding a solvent into the reaction container until the 8-alkynyl naphthylamine compound is totally dissolved; putting the reaction container in an oil bath at 0-100 DEG C, and stirring for reacting for 0-24h; cooling to room temperature; filtering with kieselguhr or silica gel powder; flushing the filter residue with dichloromethane for 2-4 times; combining the filtrates and performing reduced-pressure distillation to remove the solvent; separating with a silica gel chromatographic column, and performing reduced-pressure distillation to obtain the polysubstituted benzo[c, d] indole compound. According to the synthesis method disclosed by the invention, the polysubstituted benzo[c, d] indole compound is synthesized by a one-pot one-step process, the processes of intermediate separation and purification are reduced, and the operation method is simple; moreover, the reaction conditions are mild, the reaction selectivity is high, the yield is high, the substrate is widely applicable, the reaction raw materials are simple and easily available, and the production cost is low; the synthesismethod is suitable for small-scale preparation in a laboratory as well as industrial large-scale production.

The invention discloses a preparation method of an isoquinolinone derivative, which comprises the following steps: weighing an 8-alkynyl-1-aryl naphthylamine compound and a catalyst according to a molar ratio of 1:(0.1-1.5), putting the 8-alkynyl-1-aryl naphthylamine compound and the catalyst into a reaction vessel, and adding a solvent into the reaction vessel until the 8-alkynyl-1-aryl naphthylamine compound is completely dissolved; and placing the reaction container at 80-150 DEG C, carrying out a stirring reaction for 0-36 h, and carrying out post-treatment to obtain the product polysubstituted isoquinolinone compound. The method is simple in operation method, mild in reaction condition, good in reaction selectivity, high in yield, convenient to separate and purify, wide in substrate universality, simple and easily available in reaction raw materials, low in production cost and good in application prospect.

The invention discloses a refining purification method for a glyphosate by-product industrial salt. The treatment method comprises the following steps: (a) performing concentration crystallization ona glyphosate diluted mother liquid to obtain a crude-product salt; (b) performing primary stirring beating washing on the crude-product salt to obtain a first-stage crude-product salt and a primary washing liquid; and (c) performing secondary stirring beating washing on the first-stage crude-product salt by adopting glyphosate production process water, and performing filtration separation on the washed slurry in a centrifugal machine to obtain secondary washing water; and performing spraying by adopting glyphosate production process water in the centrifugal machine to obtain a refined industrial salt and tertiary washing water. The treatment method provided by the invention can treat the glyphosate by-product industrial salt to obtain the refined industry salt, and compared to the prior art, the method provided by the invention not only improves quality of the industrial salt, but also avoids production of new three wastes (waste water, waste gas and solid waste).

The invention discloses a synthesis method of polysubstituted sulfur-containing or selenium-containing benzo[c,d]indole compounds. Firstly, 8-alkynylnaphthylamine compounds, thioether (diselenide), acatalyst and an additive are placed in a reactor, a solvent is added to the reactor until 8-alkynylnaphthylamine compounds and thioether (diselenide) are completely dissolved, then, the mixture is heated to react, after the reaction, aftertreatment is performed, and the polysubstituted sulfur-containing or selenium-containing benzo[c,d]indole compounds are obtained. The polysubstituted sulfur-containing or selenium-containing benzo[c,d]indole compounds are synthesized with a one-pot one-step method, the reaction operation is simple, intermediate separation and purification processes are reduced, the reaction condition is mild, the reaction selectivity is good, the yield is high, the substrate universality is wide, the reaction materials are simple and easy to obtain, the production cost islow, and the method is applicable to large-scale industrial production as well as small-scale laboratory preparation.

The invention discloses a method for separating and culturing muskrat scent gland secretory cells in vitro and its application, and belongs to the technical field of cell biology. In the present invention, the scent gland tissues of muskrats in the fragrance secretion stage are taken, and immediately placed in a 4°C pre-cooled phosphate buffer solution containing penicillin and streptomycin for cleaning treatment, and the peripheral tissues of the scent glands are removed. The scented gland tissue was washed with a 4°C pre-cooled phosphate buffer solution containing penicillin and streptomycin, then centrifuged, digested with type II collagenase, centrifuged after digestion, and the precipitated tissue was cultured and separated. The invention simplifies the in vitro separation and culture steps of the muskrat scent gland secreting cells, only needs to obtain uniform small pieces of scent gland tissue, and can directly carry out the culture operation, and can ensure that the growth condition of the cells is close to the microenvironment in the body. At the same time, the present invention can simply and quickly obtain secretory cells with high purity, greatly shortening the process of separating and purifying secretory cells.

The invention discloses a phospholipid-based emulsifier for an emulsion explosive and a preparation method thereof. The phospholipid-based emulsifier is prepared through performing acylation reaction on phospholipid base and acid anhydride and then performing grafting reaction with hydramine, wherein the phospholipid content in the used phospholipid base is 50-80%.The method disclosed by the invention has the following advantages: no solvent condition is needed in the reaction, the variations of the acylation rate and the reaction product acid value are tracked in the reaction process, and the ending of the reaction is judged according to the acid value. According to the method, the phospholipid-based emulsifier generated through the reaction has favorable emulsifying property and emulsifying stability; and the prepared emulsion explosive has fine explosiveness and long storage time.

The invention discloses a method for preparing halogenated aniline from halogenated nitrobenzene through catalytic hydrogenation. The method comprises the following steps: in a reaction kettle, performing liquid phase catalytic hydrogenation reaction to halogenated nitrobenzene under the action of a sulfur-doped carbon material loaded noble metal catalyst, to obtain halogenated aniline shown in the formula (II), wherein the loading quantity of noble metal in the sulfur-doped carbon material loaded noble metal catalyst is 0.1-5wt%. In the method, the catalyst has good stability, the hydrogenation halogen removal side effect can be effectively inhibited under the condition of having no added halogen removal inhibitor, and the product selectivity is high.

The invention discloses a synthetic method for indole-3-carboxaldehyde compounds. The synthetic method comprises the following steps: (1) using anhydrous dimethylformamide as a solvent, and slowly adding phosphorus oxychloride, and agitating for 30 minutes at 0 to 5 DEG C so as to obtain a Vilsmeier agent; (2) using the anhydrous dimethylformamide as the solvent, adding 2-methylaniline compounds shown in the formula (I), dropping the Vilsmeier agent prepared in step (1) at 0 to 5 DEG C, agitating for 1 to 2 hours at room temperature after dropping, and heating for reflux to react for 5 to 8 hours; and (3) cooling after the reaction, adding a saturated sodium carbonate solution to adjust to alkalinity, filtering the precipitated solid, an drying to obtain a product, and finally recrystallizing the product to obtain the indole-3-carboxaldehyde compounds as shown in the formula (II). The method disclosed by the invention is simple to operate, and low in cost of production, and is not only suitable for small-scale preparation in a laboratory, but also suitable for large-scale industrial production.

The invention discloses an electric field intensified brewing process of sea buckthorn and wolfberry health-care fruit wine. Using immobilized Saccharomyces cerevisiae, the mixed juice of sea buckthorn and wolfberry is fermented into low-alcohol fruit wine by electric field enhanced liquid fermentation, and then the sea buckthorn and wolfberry health fruit wine drink is obtained through subsequent processing. Immobilized yeast has a short adaptation period after inoculation, which can save the total fermentation time and can be reused, omitting cumbersome operations such as re-inoculation of each batch and sterilization of fermenters in batch fermentation, reducing production costs and improving production efficiency. The electric field enhanced liquid fermentation technology can promote the growth and metabolism of yeast cells, solve the problems of slow alcohol production rate, low conversion rate and long fermentation period, and can also significantly improve the quality of fruit wine, which has a wide range of practicability.

The invention discloses a method for comprehensive recovery of copper-containing sulfur-gold ore charcoal leaching solution resources. Firstly, the copper-cyanide complex in the carbon leaching solution is converted into copper thiocyanate by acidification to precipitate and recover copper, and the copper is fully released. Cyanide, in order to facilitate the recovery of hydrocyanic acid by blowing off; then selectively convert thiocyanate into cyanide by electrocatalytic oxidation, and at the same time, use the air to separate the cyanide from the solution in the form of hydrocyanic acid in time, Avoid further oxidation of cyanide; then adjust the pH of the solution to 6-9; finally recover gold, copper, cyanide, thiocyanate, etc. remaining in the water through activated carbon adsorption to realize resource recovery and further purification; The iron slag is reused as the iron salt for electrocatalytic oxidation, realizing recycling and reducing the amount of by-product slag. The method of the invention has a high comprehensive recovery rate of resources, and the treated aqueous solution has a low concentration of pollution factors, which can meet discharge standards or be directly reused.

The invention discloses a genetically engineered bacterium community based on artificial design and a construction method and application thereof. The genetically engineered bacterium community based on artificial design comprises a recombinant engineering bacterium 1 containing a GtF3 'H gene and a CPR gene and a recombinant engineering bacterium 2 containing an MpOMT gene. The construction method comprises the following steps: constructing a recombinant engineering bacterium 1 and a recombinant engineering bacterium 2; culturing into a culture solution; and mixing to obtain the genetically engineered bacterium community. In the genetically engineered bacterium community, GtF3 'H catalyzes a hydroxylation reaction of a substrate naringenin to synthesize an intermediate product eriodictyol, CPR is a cofactor for the GtF3' H to perform a catalytic function and transfers electrons of NADPH to GtF3 'H to perform a hydroxylation reaction, and MpOMT catalyzes an oxygen methylation reaction of the intermediate product eriodictyol to synthesize hesperetin.