43results about How to "Reduce separation and purification process" patented technology

A synthetic method of 2-phenyl-3-cyano benzofuran compounds is disclosed. The method includes weighing an aldehyde compound, an aryl acetonitrile compound, a catalyst and an alkali, according to a mole ratio of 1:1-2:0.2:4, adding into a reaction container, adding a solvent into the container until the aldehyde compound and the aryl acetonitrile compound are fully dissolved, putting the reaction container into an oil bath having temperature of 100-120 DEG C, reacting under stirring for 18-24 h, cooling to room temperature, adding water the volume of which is same as the volume of the solvent, extracting with dichloromethane for 2-4 times, separating by a silica column and distilling under vacuum to obtain a product that is one of the 2-phenyl-3-cyano benzofuran compounds. The method adopts a one-pot manner to produce the 2-phenyl-3-cyano benzofuran compounds, reduces processes of separation and purification of intermediates, and is simple in operation method, mild in reaction conditions, simple and easily available in raw materials, low in production cost, suitable for small-scale preparation in a laboratory and suitable for large-scale industrial production.

The process of biosynthesizing gamma-amino butyric acid includes the following steps: slant agar culture to activate Lactobacillus brevis with the preservation number of CGMCC No. 1306, inoculating to GYP or MRS seed culture medium to culture for 10-30 hr, inoculating to GYP or MRS fermenting culture medium in the amount of 0.5-5 % to stationarily culture at 25-35 deg.c for 48-120 hr to obtain fermented liquid containing thallus and centrifugally separating and collecting thallus; washing thallus with sterilized deionized water, suspending wet thallus in citric acid-disodium hydrogen phosphate buffering system with sodium L-glutamate to react for 1-10 hr, and centrifuging the reacted liquid to obtain gamma-amino butyric acid solution. The present invention has low production cost, mild reaction condition, less environmental pollution, simple process, high production efficiency, and high product purity.

The invention discloses a synthesis method of polysubstituted sulfur-containing or selenium-containing benzo[c,d]indole compounds. Firstly, 8-alkynylnaphthylamine compounds, thioether (diselenide), acatalyst and an additive are placed in a reactor, a solvent is added to the reactor until 8-alkynylnaphthylamine compounds and thioether (diselenide) are completely dissolved, then, the mixture is heated to react, after the reaction, aftertreatment is performed, and the polysubstituted sulfur-containing or selenium-containing benzo[c,d]indole compounds are obtained. The polysubstituted sulfur-containing or selenium-containing benzo[c,d]indole compounds are synthesized with a one-pot one-step method, the reaction operation is simple, intermediate separation and purification processes are reduced, the reaction condition is mild, the reaction selectivity is good, the yield is high, the substrate universality is wide, the reaction materials are simple and easy to obtain, the production cost islow, and the method is applicable to large-scale industrial production as well as small-scale laboratory preparation.

The invention discloses a method for separating and culturing muskrat scent gland secretory cells in vitro and its application, and belongs to the technical field of cell biology. In the present invention, the scent gland tissues of muskrats in the fragrance secretion stage are taken, and immediately placed in a 4°C pre-cooled phosphate buffer solution containing penicillin and streptomycin for cleaning treatment, and the peripheral tissues of the scent glands are removed. The scented gland tissue was washed with a 4°C pre-cooled phosphate buffer solution containing penicillin and streptomycin, then centrifuged, digested with type II collagenase, centrifuged after digestion, and the precipitated tissue was cultured and separated. The invention simplifies the in vitro separation and culture steps of the muskrat scent gland secreting cells, only needs to obtain uniform small pieces of scent gland tissue, and can directly carry out the culture operation, and can ensure that the growth condition of the cells is close to the microenvironment in the body. At the same time, the present invention can simply and quickly obtain secretory cells with high purity, greatly shortening the process of separating and purifying secretory cells.

The invention discloses a method for preparing halogenated aniline from halogenated nitrobenzene through catalytic hydrogenation. The method comprises the following steps: in a reaction kettle, performing liquid phase catalytic hydrogenation reaction to halogenated nitrobenzene under the action of a sulfur-doped carbon material loaded noble metal catalyst, to obtain halogenated aniline shown in the formula (II), wherein the loading quantity of noble metal in the sulfur-doped carbon material loaded noble metal catalyst is 0.1-5wt%. In the method, the catalyst has good stability, the hydrogenation halogen removal side effect can be effectively inhibited under the condition of having no added halogen removal inhibitor, and the product selectivity is high.

The invention discloses a synthetic method for indole-3-carboxaldehyde compounds. The synthetic method comprises the following steps: (1) using anhydrous dimethylformamide as a solvent, and slowly adding phosphorus oxychloride, and agitating for 30 minutes at 0 to 5 DEG C so as to obtain a Vilsmeier agent; (2) using the anhydrous dimethylformamide as the solvent, adding 2-methylaniline compounds shown in the formula (I), dropping the Vilsmeier agent prepared in step (1) at 0 to 5 DEG C, agitating for 1 to 2 hours at room temperature after dropping, and heating for reflux to react for 5 to 8 hours; and (3) cooling after the reaction, adding a saturated sodium carbonate solution to adjust to alkalinity, filtering the precipitated solid, an drying to obtain a product, and finally recrystallizing the product to obtain the indole-3-carboxaldehyde compounds as shown in the formula (II). The method disclosed by the invention is simple to operate, and low in cost of production, and is not only suitable for small-scale preparation in a laboratory, but also suitable for large-scale industrial production.

The invention discloses an electric field intensified brewing process of sea buckthorn and wolfberry health-care fruit wine. Using immobilized Saccharomyces cerevisiae, the mixed juice of sea buckthorn and wolfberry is fermented into low-alcohol fruit wine by electric field enhanced liquid fermentation, and then the sea buckthorn and wolfberry health fruit wine drink is obtained through subsequent processing. Immobilized yeast has a short adaptation period after inoculation, which can save the total fermentation time and can be reused, omitting cumbersome operations such as re-inoculation of each batch and sterilization of fermenters in batch fermentation, reducing production costs and improving production efficiency. The electric field enhanced liquid fermentation technology can promote the growth and metabolism of yeast cells, solve the problems of slow alcohol production rate, low conversion rate and long fermentation period, and can also significantly improve the quality of fruit wine, which has a wide range of practicability.

The invention discloses a method for comprehensive recovery of copper-containing sulfur-gold ore charcoal leaching solution resources. Firstly, the copper-cyanide complex in the carbon leaching solution is converted into copper thiocyanate by acidification to precipitate and recover copper, and the copper is fully released. Cyanide, in order to facilitate the recovery of hydrocyanic acid by blowing off; then selectively convert thiocyanate into cyanide by electrocatalytic oxidation, and at the same time, use the air to separate the cyanide from the solution in the form of hydrocyanic acid in time, Avoid further oxidation of cyanide; then adjust the pH of the solution to 6-9; finally recover gold, copper, cyanide, thiocyanate, etc. remaining in the water through activated carbon adsorption to realize resource recovery and further purification; The iron slag is reused as the iron salt for electrocatalytic oxidation, realizing recycling and reducing the amount of by-product slag. The method of the invention has a high comprehensive recovery rate of resources, and the treated aqueous solution has a low concentration of pollution factors, which can meet discharge standards or be directly reused.