Dominant SCAR molecule mark for wild cabbage type cole self-incompatibility and uses thereof

A Brassica napus and molecular marker technology, applied in DNA/RNA fragments, recombinant DNA technology, microbial measurement/testing, etc., can solve problems such as self-incompatibility, reduce breeding workload, and shorten breeding years Effect

Inactive Publication Date: 2008-09-03
HUAZHONG AGRI UNIV
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Problems solved by technology

These works laid the foundation for molecular marker-assisted selection (MAS) self-incompati

Method used

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  • Dominant SCAR molecule mark for wild cabbage type cole self-incompatibility and uses thereof
  • Dominant SCAR molecule mark for wild cabbage type cole self-incompatibility and uses thereof
  • Dominant SCAR molecule mark for wild cabbage type cole self-incompatibility and uses thereof

Examples

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Embodiment 1

[0044] Through screening, the applicant obtained a batch of artificially created dominant SCAR molecular markers of self-incompatibility S sites in Brassica napus, including self-incompatibility line S-site dominant markers and self-incompatibility restorer lines S-site dominant markers, wherein the self-incompatible line S-site dominant markers are respectively named SRKa, SRKb and SP11a, and their nucleotide sequences are shown in the sequence table SEQ ID NO: 1, 2, 6 ; The dominant markers at the S site of the self-incompatibility restorer line are named SLGa, SLGb and SLGc respectively, and their nucleotide sequences are shown in SEQ ID NO: 3, 4, 5 in the sequence table.

[0045] A method for screening Brassica napus self-incompatibility S site dominant SCAR molecular markers, the steps are as follows:

[0046] 1) The self-incompatibility line S-1300 of Brassica napus was used as the female parent, and the self-incompatibility restorer line Defendor of Brassica napus from ...

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Abstract

The invention belongs to the field of rape breeding, particularly relates to rape self-incompatibility complete linkage dominant SCAR labels and the application thereof. Cabbage type rape self-incompatibility S locus dominant SCAR molecular labels are produced artificially, which includes self-incompatibility S locus dominant labels and self-incompatible restorer line S locus dominant labels, wherein the self-incompatibility S locus dominant SCAR labels are named as SRKa, SEKb and SP11a, whose nucleotide sequences are displayed in sequence tables with SEQ ID NO: 1, 2, 6; the self-incompatible restorer line S locus dominant SCAR labels are named as SLGa, SLGb and SLGc, whose nucleotide sequences are displayed in sequence tables with SEQ ID NO: 3, 4, 5. The molecular labels can differentiate homozygous-compatible, hybrid-compatible and homozygous-incompatible genes. The molecular labels are used to verify the cabbage type rape self-incompatibility phenotype. The invention provides practical labels and a usage method for rape molecular breeding.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and in particular relates to an artificially created dominant SCAR molecular marker of Brassica napus self-incompatibility and its application in marker-assisted selection of Brassica napus. Background technique [0002] Compared with cytoplasmic male sterility, which is currently the main way of utilizing heterosis in Brassica napus, self-incompatible hybrids have the advantages of short breeding cycle, many restorer lines, no adverse cytoplasmic effects, and high seed production yield. One of the important ways to utilize the advantages has become one of the research hotspots of breeders at home and abroad in recent years. [0003] Brassica napus (AA) and Brassica napus (CC) are both naturally self-incompatible, while Brassica napus (AACC) is self-compatible and lacks self-incompatibility resources. Excellent self-incompatibility materials of Brassica napus can be bred through direct...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 马朝芝张幸果傅廷栋涂金星沈金雄
Owner HUAZHONG AGRI UNIV
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