Dominant SCAR molecule mark for wild cabbage type cole self-incompatibility and uses thereof
A cabbage rape, molecular marker technology, applied in DNA/RNA fragment, recombinant DNA technology and other directions, can solve problems such as self-incompatibility, and achieve the effect of reducing breeding workload and shortening breeding years
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[0044] Through screening, the applicant obtained a batch of artificially created dominant SCAR molecular markers of self-incompatibility S sites in Brassica napus, including self-incompatibility line S-site dominant markers and self-incompatibility restorer lines S-site dominant markers, wherein the self-incompatible line S-site dominant markers are respectively named SRKa, SRKb and SP11a, and their nucleotide sequences are shown in the sequence table SEQ ID NO: 1, 2, 6 ; The dominant markers at the S site of the self-incompatibility restorer line are named SLGa, SLGb and SLGc respectively, and their nucleotide sequences are shown in SEQ ID NO: 3, 4, 5 in the sequence table.
[0045] A method for screening Brassica napus self-incompatibility S site dominant SCAR molecular markers, the steps are as follows:
[0046] 1) The self-incompatibility line S-1300 of Brassica napus was used as the female parent, and the self-incompatibility restorer line Defendor of Brassica napus from ...
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