Porcine MMP20 gene segment as genetic marker of litter size trait and application

A technology of genetic markers and litter size, applied in the fields of application, genetic engineering, plant genetic improvement, etc.

Active Publication Date: 2017-12-08
应城市永盛畜牧有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the influence of MMP20 gene on reproductive performance has not been reported yet.

Method used

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  • Porcine MMP20 gene segment as genetic marker of litter size trait and application
  • Porcine MMP20 gene segment as genetic marker of litter size trait and application
  • Porcine MMP20 gene segment as genetic marker of litter size trait and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Obtaining of porcine STIM1 gene fragment and establishment of polymorphism detection method

[0021] 1. Extraction of pig genomic DNA

[0022] The test pig breeds of the present invention are large white pigs, Meishan pigs and a new strain of Chinese lean pigs DVI (abbreviated as DVI pigs). The samples come from the Institute of Animal Husbandry and Veterinary Medicine of Hubei Academy of Agricultural Sciences and Huazhong Agricultural University. The Meishan pig is a Chinese local pig breed, and the DVI pig is a new breed of large white pig and Tongcheng pig. The extraction of pig genomic DNA adopts the genomic DNA kit (operated according to the instructions of the kit) produced by Beijing Biotech Biotechnology Co., Ltd., and the specific steps are as follows:

[0023] (1) Take pig ear or tail tissue, put it into a 2mL centrifuge tube, add 200μL of lysate TL, and blow it evenly with the tip of the pipette;

[0024] (2) Add 20 μL proteinase K (20mg / ml), vigo...

Embodiment 2

[0047] Example 2 Application of the genetic markers prepared by the present invention in the association analysis of pig litter size traits

[0048] The applicant analyzed the relationship between the genetic markers prepared by the present invention and pig litter size traits in 185 Large White pig herds (from the Institute of Animal Husbandry and Veterinary Medicine, Hubei Academy of Agricultural Sciences) and a total of 497 parity reproductive trait records. The PCR direct sequencing method established in Example 1 was used for genotyping detection, and the SPSS statistical software (Statistical Package for the Social Sciences, Version 17.0) general linear model GLM was used for statistical analysis, wherein the fixed effects included field number, year and litter seasonal effect.

[0049] The results of the association analysis are summarized in Table 2.

[0050] Table 2 Statistical analysis results of pig MMP20 gene mutation and litter size traits

[0051]

[0052] T...

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Abstract

The invention belongs to the technical field of domestic animal molecular genetic marker screening, and specifically relates to a porcine MMP20 gene segment as a genetic marker of a pig litter size trait and an application. By screening, a genetic marker, as shown by SEQ ID NO:1, related to the pig litter size trait is obtained, the gene segment has a G>C base mutation at a site 257bp, and a C>T base mutation at a site 292bp; and another genetic marker, as shown by SEQ ID NO:2, related to the pig litter size trait is obtained, and the gene segment has a C>G base mutation at a site 257bp and a T>C base mutation at a site 292bp. The above two mutation sites are completely interlocked, so that the pig litter size trait can be significantly affected. The invention further discloses a screening method of the genetic marker and an application of the genetic marker in correlation analysis of the pig litter size trait. The invention provides a novel molecular breeding marker for marker assisted breeding of the pig litter size trait.

Description

technical field [0001] The invention belongs to the technical field of livestock genetic marker screening, and in particular relates to a genetic marker and application of pig litter size traits. The pig genetic marker is located on the nucleotide fragment of the pig MMP20 gene. Background technique [0002] The litter size of pigs is an important economic trait directly related to the economic benefits of the pig industry, and increasing the litter size of pigs is of great significance for improving the overall economic benefits of the pig industry. However, the sow litter size trait is a complex trait with low heritability (about 0.1), and it is difficult to genetically improve it by conventional breeding techniques. Therefore, finding key molecular genetic markers controlling pig litter size traits will be applicable to this Molecular assisted breeding for traits is of great significance for improving piglet size. [0003] MMP20 (Matrix Metallopeptidase 20, matrix metal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/12
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 吴俊静董斌科梅书棋彭先文乔木武华玉宋忠旭孙华李良华李明波刘贵生胡华
Owner 应城市永盛畜牧有限责任公司
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