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SNP marker related to Sujiang pig production traits and preparation method and application thereof

A technology for production traits and Sujiang pigs, which is applied in biochemical equipment and methods, microbial determination/inspection, recombinant DNA technology, etc., and can solve the problems of low feed conversion rate, low lean meat rate, slow growth rate, etc.

Active Publication Date: 2020-10-20
JIANGSU AGRI ANIMAL HUSBANDRY VOCATIONAL COLLEGE
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Problems solved by technology

Chinese local pigs have the characteristics of high litter size, excellent meat quality, rough feeding resistance and strong environmental adaptability, but low lean meat rate, slow growth rate and low feed conversion rate

Method used

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  • SNP marker related to Sujiang pig production traits and preparation method and application thereof
  • SNP marker related to Sujiang pig production traits and preparation method and application thereof
  • SNP marker related to Sujiang pig production traits and preparation method and application thereof

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Embodiment Construction

[0030] Below in conjunction with specific embodiment the present invention is described in further detail. Given detailed implementation and specific operation process, examples will help to understand the present invention, but the protection scope of the present invention is not limited to the following examples.

[0031] 1. Identification of genetic variation sites of Sujiang pig PLAG1 gene

[0032] (1) Reference sequence download and primer design

[0033] From Ensembl ASIA Download the pig reference genome version 11.1 PLAG1 gene (ENSSSCG00000006247) sequence SEQ ID NO: 1 ( http: / / asia.ensembl.org / index.html ), including exons, introns, and 600bp 5' flanking sequences and 3' flanking sequences, PCR primers were designed using Primer 5.0 for the exons (as shown in Table 1).

[0034] Table 1 Exon PCR primers

[0035]

[0036] (2) PCR amplification

[0037] Randomly select 100 Sujiang pig DNA samples, uniformly dilute to 50 ng / μL, take 10 μL from each sample, and mi...

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Abstract

The invention discloses an SNP marker related to Sujiang pig production traits. The locus of the SNP marker is selected from at least one of the following loci: (1) the locus mutation of the No. 4 chromosome basic group (rs334240650) of the pig reference genome 11.1 version from C to T; (2) the locus mutation of the No. 4 chromosome basic group (rs326013678) of the pig reference genome 11.1 version from A to G; and (3) the locus mutation of the No. 4 chromosome basic group (rs328675134) of the pig reference genome 11.1 version from A to G, wherein the three loci are completely linked. The correlation analysis determines that the three molecular markers are significantly related to the Sujiang pig production traits and can be used in molecular marker-assisted selection breeding of Sujiang pigs.

Description

technical field [0001] The invention belongs to the field of pig marker-assisted selection (MAS) technology and genetic breeding, and relates to the preparation and application of three molecular markers that can be used for marker-assisted selection of production traits of Sujiang pigs. Background technique [0002] With the continuous improvement of living standards, people's demand for pork is changing from quantity to quality. Chinese local pigs have the characteristics of high litter size, excellent meat quality, resistance to rough feeding and strong environmental adaptability, but low lean meat rate, slow growth rate and low feed conversion rate. In order to meet people's increasing demand for high-quality pork, there has been an upsurge in China to breed new breeds by crossbreeding Western pigs and Chinese local pigs. The breeding of Sujiang pigs began in 1996, with Jiangquhai pigs and Fengjing pigs as the basic female parent, and Duroc pig as the male parent, throu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156Y02P60/87
Inventor 徐盼赵旭庭周春宝陶勇倪黎纲朱淑斌张君胜洪渊
Owner JIANGSU AGRI ANIMAL HUSBANDRY VOCATIONAL COLLEGE
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