Female backcross method for directionally transferring silkworm plain markings variety into multilunar sex-limiting system

A spot-limited and tea-spot technology, applied in the field of animal husbandry germplasm creation, can solve the problems of low preservation of excellent traits, low accuracy rate, and complicated operation of reincarnated parents, achieving high accuracy rate, guaranteed success rate of transfer, highly feasible effect

Active Publication Date: 2021-07-09
云南省农业科学院蚕桑蜜蜂研究所 +1
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0005] This invention is to solve the limitations of the existing technology of creating a tea spot-limited system, low preservation of good traits of reincarnated parents, complicated operation and low accuracy The problem is to use the characteristics of the complete linkage of silkworm females, and adopt the directional transfer methods such as simple hybridization, backcross and self-crossing of common spot-limited varieties, basic common tea varieties and plain spot varieties, and can be accurately and quickly created by the classification selection method A tea spot-limited system that contains more genetic components of the recurrent parent Suban cultivar and retains its excellent traits

Method used

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  • Female backcross method for directionally transferring silkworm plain markings variety into multilunar sex-limiting system
  • Female backcross method for directionally transferring silkworm plain markings variety into multilunar sex-limiting system

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Embodiment 1

[0029] Embodiment one: a kind of female backcrossing method that the Bombyx mori variegated species is directionally transformed into tea spot-limited sex, comprises the following steps:

[0030] (1) Use females of common-spot-restricted varieties Crossed with the male (ZZ +p / +p L / L) of the basic pucha variety to get F 1 generation;

[0031] (2)F 1 Each generation of male and female has 1 genotype (female: p / +p + L / L, male: ZZ p / +p + L / L) and 1 phenotype each (female: deep pupa, male: pupa). They were mixed and bred until cocoons were formed in the upper cocoons, and the females were retained after cutting the cocoons and identifying pupae. Taixiong (ZZ p / +p + L / L), using the female and the reincarnated parent Suban variety male (ZZ p / p + L / + L ) cross, get F 2 generation;

[0032] (3)F 2 on behalf of p / p + L / + L , +p / p + L / + L , +p / p L / + L , p / p L / + L 、ZZ p / p + L / + L 、ZZ +p / p + L / + L 、ZZ +p / p L / + L and ZZ p / p L / + L 8 genotypes, inclu...

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Abstract

The invention discloses a female backcross method for directionally transforming a silkworm plain markings variety into multilunar sex-limiting system. The method comprises the following steps: hybridizing a female common marking sex-limited variety and a male basic common multilunar variety to obtain F1; hybridizing the F1 female and the male recurrent parent (plain marking variety) to obtain F2; backcrossing the F2 multilunar female and the male recurrent parent to obtain BC1; dividing the BC1 into a moth area type 1 and a moth area type 2 according to the phenotype number, and backcrossing the multilunar male of the moth area type 1 and recurrent parent male to obtain BC2; continuously backcrossing the backcrossed offspring multilunar female to the recurrent parent male to BCn; performing female and male selfing on BCn multilunar to obtain S1; carrying out female and male selfing on the S1 multilunar to obtain S2; randomly numbering moth areas with only two phenotypes of common multilunar and Ji multilunar, and performing male and female selfing in the moth areas to obtain S3; and S3, selecting and remaining a breeding area only having common multilunar and Ji multilunar, namely the multilunar sex-limiting system. According to the method, the characteristic of complete linkage of female silkworms and the principle of interaction of multilunar and common markings are fully utilized, the silkworm common marking variety is directionally transformed into the multilunar sex-limiting system, and the method is high in feasibility, good in character retention degree and high in practicability.

Description

technical field [0001] The invention belongs to the technical field of animal husbandry germplasm creation, and specifically relates to a female backcrossing method for directional transfection of a silkworm plain spot variety into a tea spot-limited system by using a basic pu-tea variety. Background technique [0002] In addition to the characteristics of laying eggs, female silkworms also have the characteristics of large cocoon shape, high cocoon production, and thick cocoon silk, while male silkworms can mate 2-3 times without affecting the fertilization rate of silkworm eggs, and there is still leaf silk transformation. It has the advantages of high yield, fine cocoon silk size and excellent quality. The sex determination of silkworm is female heterogamy, that is, the sex chromosome of female silkworm is ZW type, and the sex chromosome of male silkworm is ZZ type, and the W chromosome has the function of completely determining female. In order to differentiate the use ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/04
CPCA01K67/04
Inventor 廖鹏飞董占鹏代方银鲁成刘敏李继娅罗顺高李涛范永慧欧秀华李琼艳白红英杨继芬朱红涛杨文李红李刚罗智明
Owner 云南省农业科学院蚕桑蜜蜂研究所
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