Preparation and application of DNA (deoxyribonucleic acid) chip for carbapenem antibiotic drug-resistant gene detection

A carbapenem, DNA chip technology, applied in biochemical equipment and methods, microbial determination/inspection, chemical library, etc., can solve the problems of high price, many influencing factors, poor freedom of antibiotic selection, etc. High specificity and good specificity
CN104774941AInactive Publication Date: 2015-07-15INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
2 Cites 9 Cited by

Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
Publication Date
2015-07-15
Estimated Expiration
Not applicable ยท inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to preparation and application of a DNA (deoxyribonucleic acid) chip for carbapenem antibiotic drug-resistant gene detection. The preparation method comprises the following steps: preparing nine carbapenem drug-resistant gene specific primers, preparing nine carbapenem antibiotic drug-resistant gene specific probes, preparing an oligonucleotide chip, establishing a multiplex PCR (polymerase chain reaction) system, and establishing a hybrid system. The gene chip can simultaneously detect nine common carbapenem drug-resistant genes, including KPC, NDM-1, OXA-23, OXA-48, OXA-51, IMP, VIM, SIM and DIM. The DNA chip has the advantages of high speed, high accuracy, high flux and high specificity, and can provide a new detection means for drug-resistant gene confirmation and molecular epidemiological survey of carbapenem antibiotic drug-resistant phenotypes.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the preparation and application of a carbapenem antibiotic resistance gene detection DNA chip, belonging to the technical field of gene chip detection. Background technique

[0002] Carbapenem antibiotics are broad-spectrum, potent, highly stable to most ฮฒ-lactamases, and can still exert excellent antibacterial effects on cephalosporin-resistant bacteria. Bacteria do not have cross-resistance to this type of drug, so carbapenem antibiotics have strong antibacterial activity against Gram-positive bacteria, negative bacteria, and anaerobic bacteria, and they are complex in the treatment of critical infections or failure of initial antibiotic therapy. One of the commonly used antibacterial drugs for infection. Carbapenem antibiotics are well tolerated in most cases, and the incidence of adverse reactions is low. They are suitable for severe Gram-negative bacterial infections, mixed infections, drug-resistant bacterial infections,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More