High-flux fast detecting method for virus pathogen

A detection method and high-throughput technology, which is applied in the field of high-throughput rapid screening and detection of porcine epidemic virus, can solve the problems of low sensitivity and specificity, inaccurate detection results, laborious and other problems, and achieve high sensitivity and specificity , easy operation and stable test results

Inactive Publication Date: 2008-07-02
SHANXI LIFEGEN
View PDF0 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a high-throughput rapid screening and detection method for pathogens, which solves the technical problems of

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High-flux fast detecting method for virus pathogen
  • High-flux fast detecting method for virus pathogen
  • High-flux fast detecting method for virus pathogen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0039] The virus pathogen high-throughput rapid screening and detection method adopted in the present invention has the following steps:

[0040] 1. Prepare chip:

[0041] The probe design and chip spotting in the chip preparation of the present invention can adopt known techniques.

[0042] (1.1) Design of probes: Design a highly specific 60bp oligonucleotide probe according to the conserved sequence of the viral genome.

[0043] (1.2) Chip spotting: first carry out the surface modification treatment of the substrate, then fix the probes on the substrate according to the designed array, prepare an oligonucleotide chip, and perform post-processing on the chip to obtain the oligonucleotide chip.

[0044] 2. Processing of samples to be tested:

[0045] The sample processing of the invention adopts the method of indirect labeling, the labeling efficiency is high, the detection sensitivity is improved, and the cost is low.

[0046] (2.1) Design and synthesis of primers: obtain...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A high-throughput method for rapid detection of virus pathogens comprises the following steps of: (1) designing probes and spotting on a chip to obtain an oligonucleotide chip; (2) designing and synthesizing primers to obtain primers corresponding to a detected pathogen; (3) performing synchronous asymmetric PCR amplification to a detected sample with the primers corresponding to the detected pathogen to obtain a single-stranded DNA product specifically complementary with the oligonucleotide; (4) labeling a fluorescent substance on the single-stranded DNA product to obtain a fluorescent-labeled single-stranded DNA product; (5) hybridizing with the oligonucleotide chip, and cleaning the chip; and (6) scanning with a fluorescent scanner to obtain virus detection and analysis results. The invention solves the time-consuming, labor-consuming, low sensibility and specificity and inaccurate detection result problems in the detection method of virus causing porcine epidemic diseases in the prior art. The invention can be used for rapid, accurate and efficient detection of multiple genes.

Description

technical field [0001] The invention relates to a screening and detection method for virus pathogens, in particular to a high-throughput rapid screening and detection method for porcine epidemic disease virus. Background technique [0002] Oligonucleotide Microarrays (Oligonucleotide Microarrays) are designed and synthesized oligonucleotide probes of a dozen to dozens of bases that are regularly arranged and fixed on the support through a spotting instrument, and then combined with fluorescent light. The labeled target sequence is hybridized under certain conditions according to the principle of base pairing. After washing, the chip is scanned by the laser confocal fluorescence detection system, and the data is compared and analyzed by the computer. One test can quickly and accurately detect multiple genes. , Efficient detection. [0003] Porcine parvovirus (PPV), porcine circovirus (PCV), and swine fever virus (CSFV) are common pathogens that cause disease in pigs, and oft...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/70C12Q1/68
Inventor 李铮陈超高金拽王小强祁会彩黄鹤
Owner SHANXI LIFEGEN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap