Method used for simplified identification of tobacco brown spot resistance using artificial disease nursery

A tobacco red spot disease and disease nursery technology is applied in the field of light and simplified identification of tobacco red spot disease resistance in artificial disease nurseries, which can solve problems affecting the accuracy and reproducibility of identification results, high intensity of disease investigation work, and fuzzy operational details. Achieve the effect of strict standardization of pathogen base and field distribution, improvement of accuracy and reproducibility, and reliable results

Active Publication Date: 2018-03-23
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

The resources of resistance to red spot disease in tobacco are all identified and screened by this method for many years and in many places, which can objectively reflect the actual resistance level of the variety, but are easily affected by meteorological conditions such as temperature and rainfall, and the bacterial content in the field. Year-to-year fluctuations are large, and reliable conclusions can only be obtained after repeated identifications over many years
The change of its pathogenic conditions seriously affects the accuracy and reproducibility of the identification results
The national standard GB / T23224 identification of disease resistance of tobacco varieties regulates the establishment of artificial disease beds in terms of the cultivation and preparation of red spot disease inoculum, inoculation period, inoculation concentration, etc., but some technical operation details are vague, and human factors, The impact of environmental conditions is relatively large, and there are still large fluctuations in the identification results, and the work intensity of disease investigation is high

Method used

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  • Method used for simplified identification of tobacco brown spot resistance using artificial disease nursery

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Experimental program
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Effect test

Embodiment 1

[0032] Identification of Field Alternative Spot Disease Resistance of Regional Trial Varieties

[0033] Tobacco red spot disease was isolated from typical diseased spots on tobacco leaves in the field, and was verified as a pathogenic fungus by the Offset method. The disease was investigated on a leaf basis, according to the national standard GB / T 23222 Tobacco pest classification and investigation methods, Tiantou Set up 2 protection rows, remove 2-3 tobacco plants at the edge of the field, select 5 inoculated tobacco plants, and investigate all leaves. The control resistant variety is Jingyehuang, the moderately resistant variety is G28, the susceptible variety is G140, and the materials to be tested are 65 regional test varieties.

[0034] Specific steps are as follows:

[0035] A. Selection of disease nursery: Choose the temperature in the mature stage of tobacco leaves over the years at 15-30°C, the temperature difference between day and night is greater than 8°C, the av...

Embodiment 2

[0045] BC 4 f 1 Identification of Alternative Spot Disease Resistance of Strains in the Field

[0046] Tobacco red spot disease was isolated from typical diseased spots on tobacco leaves in the field, and was verified as a pathogenic fungus by the Offset method. The disease was investigated on a leaf basis, according to the national standard GB / T 23222 Tobacco pest classification and investigation methods, Tiantou Set up 2 protection rows, remove 2-3 tobacco plants at the edge of the field, select 5 inoculated tobacco plants, and investigate all leaves. The control resistant variety is Jingyehuang, the moderately resistant variety is G28, and the susceptible variety is G140. The materials to be tested are 120 BCs formed by Y3 and Beinhart1000-1 4 f 1 strain.

[0047] Specific steps are as follows:

[0048] A. Selection of disease nursery: Choose the temperature in the mature stage of tobacco leaves over the years at 15-30°C, the temperature difference between day and nigh...

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Abstract

The invention discloses a method used for simplified identification of tobacco brown spot resistance using artificial disease nursery. The method comprises following steps: disease nursery selection,intensive line-type field overall arrangement, fertilizer controlled cultivation, formation of artificial disease nursery via relatively low concentration large area spraying, moisture retention inducing, and resistance evaluation. According to the method, the characteristics of field tobacco plants, that leaves are susceptible to diseases at maturation stage, and tobacco brown spot is easily caused at an appropriate temperature and high humidity conditions, are taken into consideration, biological zones with appropriate temperature, high humidity, irrigation convenience, and excellent irrigation conditions at tobacco leaf maturation stage are taken as disease nursery; intensive line-type field overall arrangement, fertilizer controlled cultivation, and plant leaf disease susceptibility increasing are adopted, effective inducing of diseases is realized, the accuracy and the reproducibility of tobacco brown spot resistance identification are increased; follow up investigation on the disease infection process of resistant (resistance and moderate resistance) and susceptible reference varieties is taken as a key investigation. Operation is simple; labor intensity is low; both water and labor are saved; cost is reduced; efficiency is high; tobacco brown spot resistant phenotype identification is accurate; and the method is worthy of popularization.

Description

technical field [0001] The invention belongs to the technical field of tobacco disease-resistant breeding, and in particular relates to a method for identifying tobacco red spot disease resistance in an artificial disease nursery. Background technique [0002] The recommended identification method for tobacco red spot disease resistance usually adopts the disease nursery identification method, which is a disease resistance identification method formed in the tobacco field growth environment, and there are two methods: artificial inoculation and natural induction. The resources of resistance to red spot disease in tobacco are all identified and screened by this method for many years and in many places, which can objectively reflect the actual resistance level of the variety, but are easily affected by meteorological conditions such as temperature and rainfall, and the bacterial content in the field. Year-to-year fluctuations are large, and reliable conclusions can only be obt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04A01G22/45
CPCA01H1/04
Inventor 方敦煌童治军李永平肖炳光陈学军王亚辉张谊寒吴兴富杨春江
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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