Application of oidium heveae-resistant gene ROH1 to arabidopsis

A technology of encoding genes and transgenic plants, which is applied in the application field of Arabidopsis thaliana resistance gene ROH1 to rubber tree powdery mildew, which can solve problems such as environmental pollution, ineffective control of latex production, and newly-pumped leaves of rubber trees.

Active Publication Date: 2015-08-26
HAINAN UNIVERSITY
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AI Technical Summary

Problems solved by technology

If the powdery mildew disease occurs in the same year and cannot be effectively controlled, it will cause a large number of new leaves of the rubber tree to fall, making the rubber tree unable to cut normally, which will have a significant impact on the latex output of the year
[0003] Worldwide, if rubber tree powdery mildew is not effectively controlled, it will have a significant impact on latex production in that year, causing huge economic losses
At present, the control of rubber tree powdery mildew in my country is mainly based on chemical control, which has caused serious pollution to the environment.

Method used

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  • Application of oidium heveae-resistant gene ROH1 to arabidopsis
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  • Application of oidium heveae-resistant gene ROH1 to arabidopsis

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Embodiment 1

[0033] Embodiment 1, the screening of gene ROH1 related to rubber powdery mildew resistance

[0034] 1. Identification of pathways related to rubber powdery mildew resistance

[0035] Rubber powdery mildew HO-73, recorded in the following literature: Collection of rubber powdery mildew and comparison of DNA and RNA extraction methods, Guangdong Agricultural Science, 2013, No. 11, 134-139. Wan Sanlian, Liang Peng, Song Fengya, Zhang Yu, Liu Wenbo, Miao Weiguo, Zheng Fucong.

[0036] Whether the powdery mildew of the rubber tree can infect the model plant Arabidopsis, what kind of interaction is mediated on the Arabidopsis, and whether Arabidopsis can be used as a tool to study the pathogenic mechanism of the powdery mildew of the rubber tree is still unclear. Unknown.

[0037] Inoculate the leaves of Hevea GT-1 strain, Arabidopsis wild-type Col-0, and Arabidopsis mutants eds1, pad4, rar1, ndr1, npr1, NahG and sid2 by inoculating powdery mildew rubber HO-73. Ten days after in...

Embodiment 2

[0044]Embodiment 2, the application of ROH1 gene in improving plant disease resistance

[0045] 1. Acquisition of ROH1 gene

[0046] Sequence 1 in the artificially synthesized sequence listing is the ROH1 gene.

[0047] 2. Obtaining and phenotypic identification of ROH1-transformed Arabidopsis mutant plants

[0048] 1. Obtained by transfecting ROH1 Arabidopsis mutant plants

[0049] 1) Obtaining the recombinant vector

[0050] The recombinant vector pCAMBIA1300-ROH1 is a vector obtained by replacing the ROH1 gene shown in Sequence 1 in the sequence listing with the DNA between the Kpn1 and Sal1 restriction sites of the pCAMBIA1300-FLAG vector, and keeping the rest of the vector sequence unchanged, which expresses the ROH1 protein .

[0051] The pCAMBIA1300-FLAG vector is described in the following documents:

[0052] Kang Sining, Yang Fan, Li Lin, Chen Huamin, Chen She, Zhang Jie.(2015) The Arabidopsis Transcription Factor BRASSINOSTEROID INSENSITIVE1-ETHYL METHANESULFONA...

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Abstract

The invention discloses an application of an oidium heveae-resistant gene ROH1 to arabidopsis. The invention provides an application of ROH1 protein, a coding gene of the ROH1 protein or a recombinant vector containing the coding gene to the regulation and control of plant disease resistance; an amino acid sequence of the ROH1 protein is a sequence 2 in a sequence table. An arabidopsis mutant with ROH1 mutation is complemented with the gene ROH1, so that a transgenic plant is obtained and an oidium heveae-resistant phenotype of wild-type arabidopsis is recovered. Therefore, the fact proves that ROH1 is the oidium heveae-resistant gene and lays a very good foundation for breeding for disease resistance.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of Arabidopsis thaliana resistance gene ROH1 to powdery mildew of rubber tree. Background technique [0002] Rubber tree powdery mildew is a fungal disease caused by rubber tree powdery mildew (Oidium heveae). When the disease occurs, the rubber tree begins to germinate or pull out bronze leaves around the Spring Festival every year. If the temperature is cooler and the air humidity is high, if the spraying control is not taken in time, the powdery mildew will cause a large area of ​​rubber in 3 to 5 days. The young leaves of the tree are susceptible to disease. In the initial stage of young leaves being susceptible to the disease, silver-white spider web-like hyphae appeared on the front or back of the leaves, and then gradually expanded, and white powdery substances grew on the surface of the leaves, forming irregular powder spots of different sizes ( figure 1 ). ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8282
Inventor 梅双双戎伟
Owner HAINAN UNIVERSITY
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