Method for identifying seedling-stage cold-resistant phenotypes of paddy rice

A technology of rice and cold tolerance, applied in the field of identification of cold tolerance phenotype of rice seedling stage, can solve the problems of difficulty in refrigeration, low efficiency, large area of ​​artificial climate chamber and the like

Inactive Publication Date: 2017-12-05
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, low-temperature treatment is generally carried out in artificial climate chambers or incubators to identify the cold tolerance of rice seedlings, but there are some shortcomings: artificial climate chambers occupy a large area, it is difficult to achieve low temperature refrigeration, and it is difficult to carry out long-term cooling. The identification of plant seedling cold tolerance test; low temperature treatment in the incubator, due to the influence of the volume and sealing of the incubator, there are problems of unstable treatment temperature and low efficiency

Method used

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  • Method for identifying seedling-stage cold-resistant phenotypes of paddy rice
  • Method for identifying seedling-stage cold-resistant phenotypes of paddy rice
  • Method for identifying seedling-stage cold-resistant phenotypes of paddy rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1, the preparation of plant treatment device

[0068] Prepare the plant treatment device. The plant treatment device includes a support, a culture plate and a fixing part. The bracket is composed of five side plates 1; the first side plate has a horizontal groove on the upper inner surface, and the second side plate until the fourth side plate has a horizontal groove on the upper part of the two sides at the corresponding position. Groove, the inner surface top of the 5th side plate has a horizontal groove at the corresponding position (that is, the opposite horizontal grooves on every adjacent two side plates are mirror images of each other). A culture plate is inserted between every two side plates, one end of each culture plate is inserted in the horizontal groove of a certain side plate, and the other end is inserted in the horizontal groove on the side plate adjacent to the side plate. In the horizontal groove opposite to the groove. 96 cultivation ch...

Embodiment 2

[0069] Embodiment 2, using plant treatment device to identify cold tolerance of rice seedling stage under low temperature stress

[0070] The plant treatment device prepared in Example 1 was used to identify the cold tolerance of rice seedlings under low temperature stress.

[0071] The low-temperature water bath in this embodiment is prepared according to the embodiment of the utility model patent with the Chinese patent application number 201620970857.0.

[0072] The rice variety to be tested is GLA4, NIP, C32069 or C32001.

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Abstract

The invention discloses a method for identifying seedling-stage cold-resistant phenotypes of paddy rice. The method provided by the invention sequentially comprises the following steps: (1) culturing germination-accelerated paddy rice seeds to be identified, so as to obtain young seedlings of a three-leaf stage; (2) carrying out low-temperature stressing on stem growing points and parts below the same of the young seedlings obtained in the step (1); (3) carrying out recovery culture on the young seedlings obtained in the step (2); and (4) after the step (3) is completed, carrying out statistics on the survival rate of the young seedlings, and identifying the cold resistance of the paddy rice to be identified under low-temperature stress according to the survival rate. The low temperature is 18 DEG C or below. Through identifying cold resistance of indica rice, i.e., Guangluai 4# and japonica rice, i.e., Nipponbare by adopting the method, results are in full agreement with expected experimental results. Therefore, the method provided by the invention can be used for identifying the seedling stage cold resistance of paddy rice under low-temperature stress and has an important application value in screening cold-resistant varieties of plants.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for identifying cold-tolerant phenotypes of rice seedlings. Background technique [0002] Temperature has an important effect on plant growth. At present, the global climate is changing abnormally, extreme weather occurs frequently, and low temperature and chilling damage have seriously affected the agricultural production activities of crops. [0003] Rice is one of the world's three major food crops, and more than 60% of my country's population takes rice as the staple food. Because rice originated in tropical and subtropical regions, it is more susceptible to cold damage than other grains. Low temperature chilling damage may occur in different growth and development stages of rice, such as germination stage, seedling stage, booting stage, flowering stage and grain filling stage, among which the chilling stress at seedling stage and booting stage has a partic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G16/00A01G7/00A01G22/22
CPCA01G7/00A01G22/22
Inventor 种康刘栋峰罗伟徐云远唐永严
Owner INST OF BOTANY CHINESE ACAD OF SCI
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