Protein relevant to plant height, coding gene and application thereof
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A highly relevant and plant-based technology, applied in the direction of plant genetic improvement, application, plant peptides, etc., to achieve a wide range of effects
Inactive Publication Date: 2010-04-28
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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However, it has not been reported in monocot rice
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Embodiment 1
[0040] Embodiment 1, the discovery of plant height-related protein and its coding gene
[0041] 1. Rice dominant dwarf mutant 986083D and its genetic analysis
[0042] A dominant dwarf mutant 986083D obtained from anther cultured offspring of autotetraploid rice H3774 was used for research. Compared with the wild type, the mutant showed: (1) the plants were significantly shorter, the panicle length and internodes were correspondingly shortened, and the plant height decreased by about 49%; (2) the number of panicles per plant was up to 28.3, an increase of about 139.8 %; (3) The number of grains per panicle decreased by about 51.9%, and the seed setting rate was significantly reduced (13.4%); (4) The grains became smaller, with a thousand-grain weight of 11.7g, and the grains were oval; (5) The plant type was compact and the leaves were short and narrow , dark green leaves; see figure 2 .
[0043] Homozygous dwarf mutant 986083D was used as the male parent, and eight rice v...
Embodiment 2
[0082] Embodiment 2, the acquisition and identification of transgenic plants
[0083] 1. Construction of recombinant expression vector
[0084] The pBS-OsFIE1 constructed in Example 1 was digested with Xba I and Sal I, and the fragment containing the OsFIE1 gene was recovered and connected to the pCAMBIA2300 vector (CAMBI, Australia) that was treated with Xba I and Sal I. A recombinant expression vector was obtained.
[0085] The recombinant expression vector was transformed into Escherichia coli DH5α competent cells. The plasmid was extracted for enzyme digestion and sequencing detection, and the detection results showed that a recombinant plasmid containing the OsFIE1 gene shown in Sequence 2 was obtained, which was named pCAMBIA2300-OsFIE1 (the backbone vector was pCAMBIA2300, inserted between the Xba I and Sal I sites OsFIE1 gene shown in sequence 2).
[0086] Second, the acquisition of recombinant Agrobacterium
[0087] pCAMBIA2300-OsFIE1 was transformed into Agrobact...
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Abstract
The invention discloses a protein relevant to plant height, a coding gene and an application thereof. The protein provided by the invention is the protein as the following (a) or (b): (a) a protein comprising an amino acid sequence shown in a sequence 1 in a sequence table; (b) a protein which is formed by substituting and / or deleting and / or adding the amino acid sequence of the sequence 1 by one or plural amino acid residues, is relevant to the plant height and is derived from the sequence 1. The relevant protein for regulating and controlling the development of the plant height mainly controls the plant height, can cause the dwarfing of plants after being expressed, is dominant relatively to normal high plants and is an important dwarfness source when being applied to the work such as plant hybridization, seed breeding, utilization and the like. A dominant short stalk gene is adopted as the dwarfness source, which is easy to solve the problem of plant height, has more extensive sieving range of parents and is beneficial to distant hybridization comprising the selective matching of the hybridized parents between indica rice and japonica rice and between subspecies.
Description
technical field [0001] The invention relates to a plant height-related protein and its coding gene and application. Background technique [0002] Plant height is one of the important agronomic traits of rice (Oryza sativa L.). In the 1960s, the "Green Revolution" marked by crop dwarf breeding in the world has greatly increased the grain yield. The semi-dwarf gene sd-1 encoding the oxidase OsGA20ox2 of the gibberellin synthesis pathway is the main gene of the rice "green revolution". In my country, except for a few varieties containing sd-g and sd-t(t), most of the indica rice varieties contain the sd-1 gene from Aizai Zhan or Bianjiao Nante. However, the dwarf origin of japonica rice varieties is complex, and its plant height may be controlled by micro-effect polygenes. A single dwarf gene that has been used for a long time can easily lead to a narrow genetic background. In the utilization of hybrid vigor between japonica and indica, hybrid plants were higher due to indi...
Claims
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