Method for propagating stonegarlic quickly and efficiently

A fast technology for Lycoris safflower, applied in the field of artificial propagation and cultivation of plants, can solve the degradation of Lycoris safflower bulbs, restrict the breeding process of Lycoris safflower, affect the resistance of Lycoris safflower and the quality of bulbs, etc. problems, to achieve the effects of saving seedling area, reducing production costs, and stabilizing genetic traits

Inactive Publication Date: 2012-10-03
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term artificial propagation is the cause of the degeneration of Lycoris safflower bulbs, and seriously affects the ...

Method used

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  • Method for propagating stonegarlic quickly and efficiently
  • Method for propagating stonegarlic quickly and efficiently
  • Method for propagating stonegarlic quickly and efficiently

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0021] Induction and cultivation of example 1 adventitious buds

[0022] 1. Take the underground bulb of Lycoris safflower, peel off the outer scales, cut off the root, and cut off about 1 / 2 to 2 / 3 of the upper half of the bulb. Divide the bulbs with bulb discs into 4-8 pieces, shake and wash with 0.1% Amway washing liquid on the shaker for 15-20 minutes, rinse with running water for 60 minutes, and then rinse with 75% alcohol on the ultra-clean workbench for 30 seconds After washing with sterile water for 4 times, disinfect with 1% sodium hypochlorite solution for 20-30 minutes (add a few drops of Tween 80 to the sodium hypochlorite solution), rinse with sterile water for 4-5 times, and finally dry the surface of the seeds with sterile filter paper moisture.

[0023] 2. Inoculate the small pieces of sterilized Lycoris safflower bulbs directly into the adventitious bud induction medium: add in the MS basic medium, 3.0 mg / L of 6-benzyl adenine (6-BA), naphthalene acetic acid (...

example 2

[0024] Rooting, transplanting of induction of example 2 clustering buds and test-tube plantlets

[0025] 1. After cultivating for 30 days, when the sprouts grow to 2-3cm, the sprouts are reinoculated into the clump bud proliferation medium of the present invention, and the proliferation culture is carried out. The cluster bud induction medium is based on the MS medium as the basic medium, and adds 5.0 mg / L of the plant growth hormone 6-benzyl adenine (6-BA), 2,4-dichlorophenoxyacetic acid (2,4- D) 1.0mg / L, naphthaleneacetic acid (NAA) 0.5mg / L, sucrose 30g / L, carry out proliferation culture. After culturing for more than 30 days, the induction rate reaches 98%, and most of the sprouts can induce 7-8 clustered buds.

[0026] 2. Choose robust test-tube plantlets, then inoculate them on 1 / 2 MS basic medium, indole butyric acid (IBA) 1.0mg / L, sucrose 60g / L, and carry out rooting induction. After 40 days of cultivation, the rooting rate reached 86%, and the average root number rea...

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Abstract

The invention provides a method for propagating stonegarlic quickly and efficiently. The method is used for large-scale production and propagation, and then market requirements are met. The stonegarlic tissue culture comprises steps of A, sterilization of explants, B, inducement of adventitious buds, C, proliferation of adventitious buds, D, rooting culture and E, seedling exercise and transplanting. The stonegarlic is propagated through the method of plant tissue culture, then the propagation cannot be affected by external conditions and can be performed at all seasons, lands occupied by seedlings are saved, the production cost is saved, all excellent characters of a matrix can be preserved, and hereditary characters are stable. By the aid of the method, a lot of excellent test-tube plantlets can be formed in a short time, the scale and industrial production is performed, and industries such as ornamental horticulture and pharmacy can obtain a lot of raw materials.

Description

technical field [0001] The invention relates to a high-efficiency rapid propagation method for Lycoris safflower, belonging to the technical field of artificial propagation and cultivation methods of plants. Background technique [0002] Lycoris safflower (L.radiata) is a perennial herb of the genus Lycoris in the family Amaryllidaceae. There are spherical bulbs underground, covered with dark brown membranous scales. The petals are bright red, also known as "Manjusawa", which is an important ornamental plant and medicinal plant. The main medicinal components of Lycoris safflower bulbs are various lycorines and galantamines. Lycorine and its derivatives have certain anticancer activity, anti-inflammatory, antipyretic, sedative and emetic effects, and are also effective for amoebic dysentery. Galantamine is a reversible cholinesterase inhibitor, which is used for the treatment of paralysis and myasthenia gravis caused by central paralysis diseases such as Alzheimer's disease...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 汪仁贺佳夏冰彭峰江玉梅李晓丹
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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