A kind of epimedium korean tissue culture rapid propagation method

The invention provides a rapid and rapid technology for the production of Epimedium, which can be applied in the field of Epimedium breeding, can solve the problems of difficult tissue culture of Epimedium korea, high contamination rate of explants, and there is no research on the rapid propagation of Epimedium korea, and achieves a reliable technical approach. , the stability of genetic traits, the effect of improving the quality and yield of medicinal materials

Inactive Publication Date: 2016-06-01
辽宁省农业科学院微生物工程中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few studies on Epimedium Korean produced in Liaoning, and the tissue culture of Epimedium Korean is difficult. Because Epimedium Korean grows in a shady and humid environment under the forest, the bacteria carried by the underground cryptobuds of the explants are affected by the soil and climate. There are more fungi, especially endophytes, and the contamination rate of explants is higher. This is also one of the difficulties in the rapid propagation of Epimedium korean tissue culture. At present, there is no research on the rapid propagation of Epimedium korean, so , its rapid reproduction is imminent

Method used

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Effect test

Embodiment 1

[0035] A kind of Epimedium Korean tissue culture rapid propagation method, comprises the steps:

[0036] (1) Selection and disinfection of explants: In the late April of spring, wild rhizomes of Epimedium korean were taken, with complete overwintering buds, and the overwintering buds were full, and the tiller buds at the root were used as explants, and first rinsed under running water for 3 hours. Clean the surface appendages with washing powder, wash them twice with sterile water on an ultra-clean workbench, disinfect the surface with 75% ethanol for 30 seconds, and then rinse them with sterile water for 5 times; then disinfect with 0.1% mercury chloride for 2 minutes, and use sterile Rinse with water 3 times, peel off the outer protective layer with a scalpel, then soak in 0.1% mercuric chloride for 2 minutes, and finally rinse with sterile water 3 times, set aside;

[0037] (2) Preparation of culture medium:

[0038] a. Basic medium: WPM medium + 30g / L sucrose + 4.5g / L aga...

Embodiment 2

[0049] A kind of Epimedium Korean tissue culture rapid propagation method, comprises the steps:

[0050](1) Selection and disinfection of explants: In the late April of spring, wild rhizomes of Epimedium korean were taken, with complete overwintering buds, and the overwintering buds were full, and the tiller buds at the root were used as explants, and first rinsed under running water for 3 hours. Clean the surface appendages with washing powder, wash them twice with sterile water on an ultra-clean workbench, disinfect the surface with 75% ethanol for 30 seconds, and then rinse them with sterile water for 5 times; then disinfect with 0.1% mercury chloride for 2 minutes, and use sterile Rinse with water 3 times, peel off the outer protective layer with a scalpel, then soak in 0.1% mercuric chloride for 2 minutes, and finally rinse with sterile water 3 times, set aside;

[0051] (2) Preparation of culture medium:

[0052] a. Basic medium: WPM medium + 30g / L sucrose + 4.5g / L agar...

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Abstract

The invention provides a tissue-culture and rapid-propagation method of epimedium koreanum nakai. The tissue-culture and rapid-propagation method comprises the following steps: selecting explants, disinfecting, preparing a culture medium, carrying out induced culture, carrying out enriched culture, carrying out rooted culture, domesticating tissue-culture seedlings and transplanting. The tissue-culture and rapid-propagation method provided by the invention has the advantages that no limitations of the geographical environment and the seasons are caused, the genetic background is consistent, a great quantity of test-tube seedlings can be formed in a short period, the problems that the current epimedium koreanum nakai is also in a wild state, the artificial breeding and cultivation period is long, the requirement for the growth environment is strict and the large-area planting is difficult are also solved, and a reliable technical path is provided for large-scale production and planting of the epimedium koreanum nakai.

Description

technical field [0001] The invention relates to a method for propagating Epimedium, in particular to a method for rapidly propagating Epimedium korean through tissue culture. Background technique [0002] Epimedium koreanum Nakai is a plant of the genus Epimedium in the Berberidaceae family. It is a perennial herb and is an important medicinal resource in my country. Korean Epimedium is mainly distributed in the eastern part of Jilin Province and Liaoning Province, and there is also a small amount of distribution in the border areas of Heilongjiang and Jilin. Epimedium can not only invigorate kidney yang, strengthen muscles and bones, and dispel rheumatism, but also anti-aging, improve immunity, inhibit Efficacy such as tumor, it is one of the traditional Chinese medicines with the most development potential. [0003] Epimedium Korean is mainly collected from wild plant resources. With the increase of demand, the amount of collection and consumption has exceeded the regener...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 杨镇龚娜李学龙王娜陈珣刘国丽肖军曹君肖伟宋艳雨李跃
Owner 辽宁省农业科学院微生物工程中心
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