Method for inducing litsea cubeta cluster buds

A technology of Radix chinensis and explants is applied in the field of tissue culture and rapid propagation of inducing the clustered buds of the woody aromatic plant Radix vulgaris Low seedling rate and other problems, to achieve the effects of inhibiting the browning of the medium and explants, improving the disinfection effect, and stabilizing the genetic traits

Inactive Publication Date: 2009-12-02
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the existing tissue culture method can obtain a higher multiplication coefficient, it has the problems of low seedling yield and unstable survival rate after transplanting.
Therefore, it is necessary to explore and study a method of inducing clustered bud culture that can not only guarantee a certain multiplication coefficient but also ensure an effective number of sprouts, so as to solve the key problem of unstable seedling rate of Litsea Cubeba in tissue culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Get the young and tender branches of Litsea Cubeba dry base, remove the leaves, put in 3% H2O after washing with running water. 2 o 2 Soak in medium for 15 minutes, take it out and immediately transfer to 0.1% HgCl containing 5% Tween-80 2 Soak in the solution for 20 minutes, constantly brush the branches with a soft brush, then take them out and rinse them with sterile water for 5 to 6 times and dry the water with sterile absorbent paper, then cut out the stems containing nodes as explants, and quickly inoculate them on the The explants were cultured in the dark on MS medium, and the explants were transferred to new MS medium the next day.

[0021] Transfer the explants that are no longer browned on MS medium to MS+6-BA1.0mg / L+IBA0.5mg / L+GA2.0mg / L+agar 7.0g / L+sucrose 30g / L, pH6.0 on the induction medium, placed at a temperature of 25±2°C, with a photoperiod of 12h.d -1 , Induced culture was carried out in an incubator with a light intensity of 1200 lux. After 21 da...

Embodiment 2

[0025] Get the young and tender branches of Litsea Cubeba dry base, remove the leaves, put in 3% H2O after washing with running water. 2 o 2 Soak in medium for 20 minutes, take it out and immediately transfer to 0.1% HgCl containing 5% Tween-80 2 Soak in the solution for 10 minutes, constantly brush the branches with a soft brush, then take them out and rinse them with sterile water for 5 to 6 times and dry the water with sterile absorbent paper, then cut out the stems containing nodes and inoculate them on MS medium, put Culture in the dark, and transfer to new MS medium the next day.

[0026] Transfer the explants that are no longer browned on MS medium to MS+6-BA0.5mg / L+IBA0.2mg / L+GA1.0mg / L+agar 7.0g / L+sucrose 30g / L, pH6.0 On the culture medium, put it at a temperature of 25±2°C and a photoperiod of 12h.d -1 , Induced culture was carried out in an incubator with a light intensity of 1200 lux. After 21 days, induced shoots were obtained.

[0027] After 30 days, cut out ...

Embodiment 3

[0030] According to the method of Example 1, only the hormone concentration in the subculture stage was reduced. Specific method: After 30 days of multiplication and culture, cut out the section-containing stem section of the bud and inoculate it into MS+6-BA1.0mg / L+IBA0.3mg / L+GA1.0mg / L agar 7.0g / L+sucrose 30g / L, pH6. 0 medium; after 30 days, transfer the buds to MS+6-BA2.0mg / L+IBA0.3mg / L+agar 7.0g / L+sucrose 30g / L pH 6.0 medium in the same way for subculture. The temperature is 25±2°C, the photoperiod is 14h.d-1, and the light intensity is 2000lux; such alternate culture, the multiplication coefficient is more than 4 times, and the sprouts obtained are strong and neat.

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Abstract

The invention relates to a method for inducing clustered buds of Litsea cubeba, which uses the nodular stem section of Litsea cubeba as an explant, soaks it in 3% H2O2 and 0.1% HgCI2 solution containing 1 to 5% Tween-80, and Constantly brushing with a soft brush instead of alcohol disinfection, high-efficiency sterilization while ensuring a sufficient number of effective explants; at the initial stage of inoculation, the method of converting medium and dark culture is used to control the browning of materials; bud induction culture Adding GA in the growth stage, and using the alternate use of proliferation medium and induction medium in the proliferation culture stage, the bud germination time is advanced, and the buds enter the proliferation state quickly, and the sprouts obtained are strong and neat. Adopting the present invention to induce breeding of litsea cubeba is not affected by external conditions and can be carried out in all seasons, which not only saves the area occupied by seedlings, reduces production costs, but also preserves all the good traits of the mother body, and the genetic traits are stable. The invention can form a large number of excellent test-tube seedlings in a short period of time for large-scale and factory production.

Description

technical field [0001] The invention relates to a method for inducing clustered buds of litsea cubeba, in particular to a tissue culture rapid propagation method for inducing clustered buds of woody aromatic plant litsea cubeba. Background technique [0002] Litseacubeba is the collective name of many plants in the genus Zingiberaceae of Lauraceae. There are more than 200 species in the world, and 72 species, 18 varieties and 3 variants in my country, mainly distributed in the south of the Yangtze River. Its aliases are Litsea jasmine (Zhejiang), Litsea cypress (Guangdong), Sai camphor tree (Fujian), Fragrant leaves (Hunan), and Mujiangzi (Guangxi). The volatile aromatic oil obtained by distillation is called litsea cubeba oil, which is a bulk essential oil for export exchange. Litsea cubeba essential oil is mainly used to extract citral, with a content of 70%. It is the main raw material for the manufacture of ionone, methyl ionone, ethyl ionone and vitamin A, and is used ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 张艳玲姚雷王维鹏张静懿高远陆伟芳陆燕李昂
Owner SHANGHAI JIAOTONG UNIV
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