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Fast propagation method of cannabis for inductrial use

An industrial use, cannabis technology, applied in the field of artificial propagation and cultivation of plants, can solve problems such as restricting development, reporting, and no shoot tip propagation, and achieve the effects of saving seedlings occupying land, reducing production costs, and stabilizing genetic traits.

Inactive Publication Date: 2007-01-03
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the establishment of cannabis tissue culture regeneration system, and there is no report on shoot tip propagation
Thus, to a certain extent, the in-depth study of cannabis in various research fields has been restricted, and its development has been restricted.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1 Take hemp seeds with uniform grains, shake and wash them with 0.1% Amway washing liquid on a shaker for 15 minutes, rinse them with running water for 30 minutes, then rinse them with 75% alcohol on an ultra-clean workbench for 25 seconds, and wash them 4 times with sterile water. Sterilize with "84" disinfectant solution containing 5% available chlorine for 10 minutes, rinse with sterile water until the water body is clear, and finally dry the water on the seed surface with sterile filter paper.

[0020] 2. Inoculate the sterilized hemp seeds directly into the following medium: 1 / 2 MS basic medium, sucrose 5g / L, adjust the pH value to 5; culture temperature 24°C, light intensity 1000lx, light time 10h, light culture.

[0021] 3. Cut off the stem tip of the aseptic seedling that has been germinated and cultivated for 20 days with scissors, and inoculate it into the medium for cultivating lateral buds of the present invention. The specific formula is to add phytohormone ...

Embodiment 2

[0024] 1 Take hemp seeds with uniform grains, shake and wash them with 0.5% Amway washing liquid on a shaker for 20 minutes, rinse them with running water for 60 minutes, then rinse them with 75% alcohol on an ultra-clean workbench for 35 seconds, and wash them with sterile water for 4 times. Sterilize with 0.1% mercuric chloride for 15 minutes, rinse with sterile water until the water body is clear, and finally dry the water on the surface of the seeds with sterile filter paper.

[0025] 2 Inoculate the sterilized hemp seeds directly into the following medium: MS basic medium, sucrose (edible sugar) 10g / L, control the pH value to 7.5; culture temperature 25°C, light intensity 3000lx, light time 10-16h, Carry out light cultivation.

[0026] 3 Cut off the stem tip of the aseptic seedlings that have been germinated and cultivated for 30 days with scissors, and inoculate them into the medium for cultivating lateral buds of the present invention. The specific formula is to add phy...

Embodiment 3

[0029] 1 Take hemp seeds with uniform grains, shake and wash them with 0.3% Amway washing liquid on a shaker for 17 minutes, rinse them with running water for 50 minutes, then rinse them with 75% alcohol on an ultra-clean workbench for 30 seconds, and wash them with sterile water for 4 times. Sterilize with "84" disinfectant containing 5% available chlorine for 12 minutes, rinse with sterile water until the water body is clear, and finally use sterile filter paper to dry the moisture on the surface of the seeds.

[0030] 2. Inoculate the sterilized hemp seeds directly into the following medium: 1 / 2 MS basic medium, sucrose 10g / L, adjust the pH value to 5.8; culture temperature 25°C, light intensity 2000lx, light time 13h, light culture.

[0031] 3 Cut off the stem tip of the aseptic seedlings that have been germinated and cultivated for 22 days with scissors, and inoculate them into the medium for cultivating lateral buds of the present invention. The specific formula is to add...

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PUM

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Abstract

The fast propagation method of hemp for industrial use is one hemp tissue culture process including the steps of sterilizing seed, obtaining bacteria-free seedling, culturing lateral bud and rooting culture. The hemp tissue culture process has no limitation of seasons, saving in seedling culture field, low production cost, capacity of maintaining all the excellent characteristics of the mother plant and stability of the genetic characteristics. The present invention can form great amount of tube seedling in short period for mass planting of hemp.

Description

technical field [0001] The invention relates to a rapid propagation method of hemp for industrial use, and belongs to the technical field of artificial propagation and cultivation methods of plants. Background technique [0002] Marijuana (Cannabis sativa L.), the cannabis for industrial use is also known as hemp, which belongs to the Cannabis family Cannabis genus annual herb, with tall plants and dioecious plants. Native to central Asia, it was one of the earliest crops planted by humans, and has now spread all over the world. [0003] Hemp seeds are rich in protein, multivitamins and trace elements, and can be squeezed for oil and eaten. The roots, bark, leaves, flowers and seeds of cannabis can be used as medicine. In addition, hemp fiber can also be used to make paper, rope and various textiles. In particular, hemp fabrics have good thermal performance, and are warm in winter and cool in summer, and the fibers are soft, so they are very suitable for personal clothing...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01C1/00A01G7/00
CPCA01H4/005
Inventor 夏冰李凝佟金凤高南
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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