Open type plant tissue culture seedlings-raising method

An open technology of plant tissue culture, applied in the field of open plant tissue culture and industrialized seedling cultivation, can solve the problems of immature large-scale production technology, difficult to solve medium pollution, cumbersome test tube production technology, etc., and achieve the omission of disinfection procedures, The effect of low price and reduction of production cost

Inactive Publication Date: 2009-06-17
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are four defects in this tissue culture method: the pollution of the medium is difficult to solve, the cost of test tube seedlings is too high, the large-scale production technology is immature and the test tube production technology is cumbersome
One of the reasons is that there is no antibiotic that is effective against all bacteria, and the efficacy period is short; the second is that some antibiotics and preservatives can directly damage plant tissues by their bactericidal and antibacterial ions at effective concentrations.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment one Konjac open tissue culture

[0032] Konjac open tissue culture is carried out according to the following steps:

[0033] (1) Preparation of antibacterial agent:

[0034] ① Preparation of garlic extract

[0035] 1 kg of fresh white-skinned garlic after peeling, after washing, add 300 ml of water, beat with a beater for 20 minutes to obtain garlic paste, pour it into a sealed tank, add 0.02mol / L hydrochloric acid dropwise and keep stirring until the pH of garlic paste = 6, Seal and stand at a temperature of 40° C., allowing the endogenous alliin in the mashed garlic to enzymatically hydrolyze alliin for 4 hours to produce allicin. After the enzymatic hydrolysis is completed, transfer the garlic pulp to a stirring tank, add 4L of 95% edible ethanol, and after fully stirring, filter the plate and frame to obtain the allicin extract, transfer it to a vacuum decompression concentration tank, and distill it to dryness under reduced pressure at 60°C. , to obta...

Embodiment 2

[0045] Example 2 Germination of Glycyrrhizae glabra, Glycyrrhiza inflata and Glycyrrhiza uralensis seeds under open conditions

[0046] Seed germination of Glycyrrhiza glabra, Glycyrrhiza inflata and Glycyrrhiza uralensis under open conditions is carried out according to the following steps:

[0047] (1) Preparation of antibacterial agent:

[0048] According to the preparation method described in Example 1, garlic extract, coptis extract and honeysuckle leaf extract were prepared, and the three extracts were mixed according to the 5 ratios described in Attached Table 2 to prepare 5 antibacterial agents .

[0049] (2) Preparation of antibacterial medium

[0050] Prepare 5 parts of 1L MS medium, and add 1% agar to it, which is the seed germination medium. Add 5 kinds of antibacterial agents described in (1) respectively, and the addition amount is 4g / L, is mixed with 5 kinds of solid bacteriostatic medium. Pack in transparent plastic cups, seal the container with plastic wra...

Embodiment 3

[0053] Embodiment 3 Glycyrrhizae open tissue culture seedling cultivation

[0054] Glycyrrhiza open tissue culture seedling cultivation is carried out according to the following steps:

[0055] (1) Preparation of antibacterial agent:

[0056] ① Preparation of licorice root extract

[0057] Accurately weigh 1 kg of dried licorice rhizome, grind it through a pulverizer and pass through an 80-mesh sieve, place the licorice powder in an extraction tank, add 3L 50% industrial ethanol, extract ultrasonically for 1 hour, filter through a plate frame to obtain a licorice flavonoid extract, and transfer it to Distilled to dryness under reduced pressure at 55° C. in a vacuum vacuum concentration tank to obtain a licorice root extract, wherein the purity of licorice flavonoids is equal to 70%.

[0058] ② Preparation of osmanthus extract

[0059] Accurately weigh 1 kg of dried sweet-scented osmanthus, add 500 ml of 90% ethanol, and desorb for 15 minutes. Then add 4L of 80% ethanol to ...

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Abstract

The present invention relates to an open type plant tissue seedling cultivation method pertaining to the agroforestry field of plant tissue seedling cultivation technology. The method can be performed in a nonsterile natural environmental condition in a room temperature and the method comprises steps of adding plant extracts bacteriostatic agent in a culture medium, in each 1L culture medium, adding bacteriostatic agent of 4-15g, manufacturing a bacteriostasis culture medium, and performing an inoculated culture by utilizing the bacteriostasis culture medium. The bacteriostatic agent comprises two kinds one of which comprises garlic extracts, coptis root extracts and honeysuckle leaf extracts with a mass percent of 20-100%:0-40%:0-50%; the other one of which comprises liquorice root extracts, osmanthus extracts and lycoris radiata extracts with a mass percent of 10-100%:0-80%:0-50%. By utilizing the method of the present invention, the plant tissue seedling cultivation process can be divorced from a stringent axenic operation environment and can be performed in an open nonsterile environment, therefore, links in the plant tissue seedling cultivation are simplified radically, the working efficiency is improved and the breeding cost is greatly reduced.

Description

technical field [0001] The invention belongs to the plant tissue culture seedling raising technology in the field of agriculture and forestry, and in particular relates to an open plant tissue culture factory seedling raising method. That is, under the condition of adding a bacteriostatic agent formulated with plant extracts to the medium, the process of plant tissue culture and seedling cultivation is separated from the strict aseptic operating environment, especially without the need for high-pressure sterilization, and can be used in an open bacteria-carrying environment. Plant tissue culture and seedling cultivation fundamentally simplifies the process of plant tissue culture and seedling cultivation, improves the efficiency of seedling cultivation, and greatly reduces the cost of plant tissue culture and seedling cultivation. Background technique [0002] Plant tissue culture is a plant propagation technology developed on the basis of plant physiology at the beginning o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A61L2/16
Inventor 余龙江杨英何峰金文闻季家兴
Owner HUAZHONG UNIV OF SCI & TECH
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