Fast tissue culture reproducing method of actinidia eriantha

Abstract

The invention discloses a fast tissue culture reproducing method of actinidia eriantha, belonging to the technical field of plant tissue culture. The method comprises the following steps: (1) tissue culture medium preparation; (2) explant selection and sterilization; (3) inducing culture; (4) proliferation culture; (5) rooting culture; (6) tissue culture plantlet acclimatization and transplant, and the like. The fast tissue culture reproducing method has high culture medium pertinence and good applicability, the inductivity of an initial sprout of an explant reaches more than 80 percent, the proliferation rate of a successive sprout reaches 2-7 times, the plantlet height can reach 2-5 centimeters for 20-30 days, the quality of tissue culture plantlets is enhanced, the rooting rate reachesmore than 95 percent, and the transplant survival rate reaches more than 95 percent. The invention can be popularized and applied to fruit tree plantlet enterprises.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture and rapid propagation method of the Actinidia genus Actinidia plant. Background technique [0002] Actinidia eriantha Benth., also known as white-haired kiwifruit and hairy wax gourd, is a unique species of kiwifruit in China, mainly distributed in the south of the Yangtze River in my country. The fruit of kiwi fruit is rich in vitamin C and various active substances. Its vitamin C content is as high as 568.9~1137.0mg / 100g. Immunity and other functions; at the same time, compared with other kiwifruit species, its peel is very easy to peel off. The above-mentioned excellent comprehensive properties have attracted extensive attention of kiwifruit researchers and commercial producers. But the lack of seedling supply of this variety has become one of the limiting factors of current development. Therefore, the rapid propagation method of this variety see...

AI Technical Summary

Problems solved by technology

However, the speed of propagating high-quality seedlings by grafting is relatively slow, and the survival rate of cutting propagation is relatively low and it is easy to cause the plants to be infected with bacteria. Breeding causes breed degeneration

The research on Actinidia chinensis is still in its infancy, and it is a new generation of kiwifruit that is currently being developed. There are few reference materials, such as Zhang Yuanji, Mu Xijin, Cai Qigui, etc. Protoplast regeneration plants of Actinidia chinensis[J]. , 1995, 37(1): 48-52; Zhang Yuanji, Qian Yingqian, Cai Qigui, etc. Chromosomal number variation and cell multinucleation in root tip regenerated plants of Actinidia trichomes protoplasts[J]. Acta Botanica Sinica, 1997, 39(2) : 102-105, they obtained regenerated plants through protoplast culture; another example is WANG T, ATKINSON RG, JANSSEN BJ.The choice of Agrobacteriuim strain for transformation of kiwifruit[J].Acta Horticulturae, 2006(a), 753-759; WANG T, RANY, ATKINSON RG, GLEAVE AP, GOHEN D. Transformation of Actinidia eriantha: A potential species for functional genomics studies in Actinidia[J]. Plant Cell Rep, 2006(b), 25: 425-431; their research Although the genetically transformed plants of Actinidia chinensis have also been obtained, their research purposes, explants used, and culture regeneration approaches are completely different from those of the present invention.