Full-wavelength light source controllable biological culture device

A biological culture, full-wavelength technology, applied in tissue cell/virus culture devices, biochemical cleaning devices, enzymology/microbiology devices, etc., can solve problems such as lack of scientific instruments, and achieve simple structure, simple and convenient operation , the effect of increasing the growth rate

Inactive Publication Date: 2012-05-02
HUBEI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In particular, the tissue culture or cultivation of rare medicinal plants or the photoreaction research of cell fermentation is of great significance, but there is no scientific instrument that meets the needs of this research.

Method used

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  • Full-wavelength light source controllable biological culture device
  • Full-wavelength light source controllable biological culture device
  • Full-wavelength light source controllable biological culture device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Such as figure 1 As shown, a biological culture device with a full-wavelength controllable light source includes a cabinet 12 and a culture bottle 13 . The first three-dimensional LED lighting panel controller 1, the second three-dimensional LED lighting panel controller 2, the third three-dimensional LED lighting panel controller 3, the fourth three-dimensional LED lighting panel controller 4, and the fifth three-dimensional LED lighting Panel controller 5, temperature, humidity and ultraviolet lamp controller 6 are respectively fixed on the inner wall of the box, and its digital display and parameter setting panel are fixed on the top outside the box. The heater 7a, compressor 7b, and humidifier 8 are respectively fixed on the bottom plate of the box body 12, and the inner wall panel (quartz panel) 9 covers the LED light source board, and is respectively fixed on the box body 12 panel by screws. The LED light source board 10 is fixed on the corresponding box body 12 ...

Embodiment 2

[0041] A laboratory application method of a full-wavelength controllable light source biological culture device:

[0042] Biomaterial: Rhodiola rosea callus

[0043] Medium: Optimized Rhodiola rosea callus medium.

[0044] Research on photoreaction conditions:

[0045] 0-24 hours after callus inoculation: dark culture, cell adaptation period.

[0046] 2-30 days after inoculation: 620-630 nanometer light treatment, surface light intensity: 400LUX (lux), light wave conditions for rapid cell division and growth, callus can grow rapidly. 30-60 days: 420-440 nanometers 50% + 620-630 nanometers 50% (50% means that the two light sources each account for half to form a mixed light source and illuminate at the same time, the same applies below), or 320-350 nanometers 40% + 620- 630nm 60%, or 280-320nm 10%+620-630nm 90% light treatment, surface light intensity: 400LUX, optimized light conditions for Rhodiola seedling differentiation and rooting. It can be repeatedly optimized to obt...

Embodiment 3

[0048] A full-wavelength controllable light source biological culture setting laboratory application method:

[0049] Biomaterial: Cordyceps militaris ( Cordyceps militaris ) strain

[0050] Medium: Cordyceps militaris liquid medium

[0051] Light-controlled fermentation procedure:

[0052] 0-48 hours after inoculation: culture in the dark to allow the strains to adapt and recover.

[0053] 48-168 hours after inoculation: 620-630 nanometers, or 600-620 or 630-640 nanometers or 650-660 nanometers light treatment, surface light intensity: 400LUX, repeated optimization can obtain the best mycelial growth and adenosine biosynthesis Optimum light conditions.

[0054] 168-288 hours after inoculation: 380-400 nm or 400-410 nm or 410-420 nm or 420-440 nm light treatment, surface light intensity: 400LUX, repeated optimization can obtain the best light wave for the biosynthesis of cordycepin and carotenoids condition.

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Abstract

The invention discloses a full-wavelength light source controllable biological culture device. The device comprises a case body the periphery of which is provided with a heat insulating layer, optical wavelength controllable LED (light emitting diode) lamp bead panels arranged on the inner top part and four lateral sides of the case body, temperature and humidity sensors arranged in the case body, a heater and a humidifier arranged at the bottom of the case body, and a miniature quiet compressor. According to the full-wavelength light source controllable and quartz culture flask, biological photo-reaction researches can be performed in plant tissue culture, cell culture, microbiological culture and animal culture ways, the operation is easy, and the light source can be subjected to wavelength combination and illumination time program setting according to different requirements.

Description

technical field [0001] The technology of the present invention relates to the field of biological culture, in particular to a plant material (including callus and seedling) culture device with a full-wavelength controllable light source, which is suitable for light control of biological cells or tissues or plant photoreaction research, physiological metabolism research or Physiological studies of animal light responses. Background technique [0002] A large number of scientific studies have proved that the vegetative growth of autotrophic biological cells and the accumulation of secondary metabolites are oriented by the wavelength under the irradiation of a certain wavelength of light source, and the metabolic pathways of heterotrophic biological cells will also change under the stimulation of special wavelength light sources. lead to the accumulation of some biologically active substances. This feature of living cells can be used for industrial production of biologically a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/38C12M1/36A01G9/20
CPCC12M41/34C12M31/10C12M3/00C12M41/12C12M41/06C12M1/00C12M21/02
Inventor 董静洲郑小江
Owner HUBEI UNIV FOR NATITIES
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