Butterfly orchid anti-browning tissue culture method and anti-browning culture medium

The technology of a culture medium and a browning group is applied in the field of plant tissue culture, which can solve the problems of unenvironmental protection, incompleteness, and few anti-browning methods, and achieve the effects of cost saving, nutritional health, and low browning rate.
CN103202233AInactive Publication Date: 2013-07-17ANHUI AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ANHUI AGRICULTURAL UNIVERSITY
Publication Date
2013-07-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention mainly relates to the field of plant tissue culture and particularly relates to a butterfly orchid anti-browning tissue culture method and an anti-browning culture medium. The tissue culture method comprises the steps of explant disinfection, primary culture, proliferation and differentiation culture, rooting culture, seedling hardening, transplanting and the like, wherein in the primary culture and the proliferation and differentiation culture, extracted solutions of calendulas, lemons, tomatoes, rosemary and the like are added into a corresponding culture medium so as to effectively prevent and treat a common browning problem in butterfly orchid tissue culture; and meanwhile, different types of cutting manners are used for helping to prevent and treat the browning problem at a differentiation phase, so as to form a compound anti-browning method. The method disclosed by the invention has the advantages of high stability, great success rate, greenness and environmental friendliness, and can annularly and repeatedly produce.
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Description

Technical field

[0001] The invention mainly relates to the field of plant tissue culture, in particular to a phalaenopsis anti-browning tissue culture method and an anti-browning culture medium. Background technique

[0002] Browning of explants is an important problem encountered in plant tissue culture. Studies have shown that the main cause of browning is that polyphenol oxidase acts on the natural substrate phenols to form quinones. The addition of antioxidants and adsorbents can better control browning.

[0003] In view of these causes of browning, earlier studies have shown that activated carbon can prevent browning, because the principle of activated carbon to prevent browning is the adsorption of activated carbon, and the adsorption of activated carbon itself is limited, that is It is impossible to add a large amount of activated carbon to each bottle, so the adsorption effect of a small amount of activated carbon is relatively small. Once the adsorbed harmful components a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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