A method for inducing proliferation of fritillary fritillaria callus

A technology of callus induction and callus, which is applied in the field of plant tissue culture, can solve the problems of unsatisfactory induction effect, achieve good quality and performance of developing seedlings, shorten the induction period, and the method is simple and easy to implement

Inactive Publication Date: 2016-06-08
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are few studies on the tissue culture of Fritillaria anhui, based on the differences among various Fritillaria species, the induction methods applicable to callus induction of different Fritillaria due to differences in their growth characteristics also have certain differences. When the existing Fritillaria tissue culture technology is applied to the callus induction of Fritillaria anhui, the induction effect is not ideal

Method used

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  • A method for inducing proliferation of fritillary fritillaria callus
  • A method for inducing proliferation of fritillary fritillaria callus
  • A method for inducing proliferation of fritillary fritillaria callus

Examples

Experimental program
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Effect test

Embodiment 1

[0049] This example is used to illustrate the method for inducing proliferation provided by the present invention.

[0050] (1) Dig out the fritillaria bulbs that are in the vegetative growth period in the greenhouse flower pots of Beilin Science and Technology Flower Engineering Technology Center.

[0051] (2) Peel off the old bulbs, roots and other debris on the above-mentioned bulbs, and then soak them in a solution containing detergent for 20 minutes. During this period, they should be shaken continuously, and then rinse with tap water for 90 minutes, and then put the bulbs Use 75% alcohol to disinfect the surface for 60 seconds on the ultra-clean workbench, rinse with sterile water for 3 times, then treat with 0.1% mercury chloride for 10 minutes, then treat with 2% sodium hypochlorite aqueous solution for 15 minutes, rinse with sterile water for 3 times, each time 5 minutes each time, then dry the surface moisture with sterile filter paper, and set aside;

[0052] (3) T...

Embodiment 2

[0059] The same method as in Example 1 is used to induce proliferation of callus, the difference is that the induction medium used is MS+0.5mg / L6-BA+4.0mg / LNAA, and the induction medium is added according to the mass volume The specific concentration is 30g / L of sucrose, 6.5g / L of agar, and the pH value of the induction medium is 5.95.

[0060] The average number and proliferation of the induced dense callus were counted, and the results are listed in Table 1.

Embodiment 3

[0062] The same method as in Example 1 is used to induce proliferation of callus, the difference is that the induction medium used is MS+0.5mg / L6-BA+2.0mg / LNAA, and the induction medium is added according to the mass volume The specific concentration is 30g / L of sucrose, 6.5g / L of agar, and the pH value of the induction medium is 5.82.

[0063] The average number and proliferation of the induced dense callus were counted, and the results are listed in Table 1.

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Abstract

A related fritillaria anhuiensis callus induced propagation method comprises the following steps: cutting a fritillaria anhuiensis bulb at the growth period into small diamonds, inoculating to an induction medium to form callus through induction, and then inoculating to a propagation medium for propagation and further for obtaining compact callus. The induction medium employs MS medium as a basal medium and contains 6-BA and NAA. The provided method is simple and practicable, shortens the induction period of callus, improves the induction success rate, and has the callus rate of 70-83.33% and the embryogenic callus rate of 80.95-84%. Also, the callus obtained according to the provided induced propagation method has relatively good quality and growth seedling-forming performance.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for inducing and proliferating callus of fritillary fritillaria. Background technique [0002] Fritillaria fritillaria (F. anhuiensis) is a perennial bulbous plant belonging to the genus Fritillaria L. of Liliaceae, which has high medicinal value and ornamental value. It is naturally distributed in the Dabie Mountains and Jianghuai Hills in Anhui Province. It has a small adaptation range, and it likes a cool climate and moist soil, but is afraid of stagnant water. When the temperature exceeds 30°C, the aboveground part will wither. At present, it is mostly cultivated in Jinzhai, Shucheng, and Huoshan in Anhui. However, as a commonly used medicinal material, it is in great demand, so it has been in short supply for a long time. [0003] The conventional propagation method of fritillary fritillary is mainly by sowing and dividing into bulbs. Its reproduction ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 于晓南郝丽红程堂仁张启翔
Owner BEIJING FORESTRY UNIVERSITY
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