Tissue culture method of brunnera macrophylla "Hadspen Cream"

A Huddersburn, tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of blank, low reproductive efficiency, and large-scale production limitations of C. The effect of vigorous division activity, improving reproductive efficiency, and improving the success rate of induction

Active Publication Date: 2021-02-19
BEIJING GREEN GARDEN GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the best breeding season for Buglossia cordata "Hadsbourn Ice Cream" is in early spring, and the main breeding methods are seeding and branching, and the breeding efficiency is not high.
That is, at other times of the year except early spring, the mass production of this variety is limited
[0004] In view of the related technologies mentioned above, the inventor believes that relying on the current breeding method of Buglossia cordata "Hadesben Ice Cream" obviously limits the application of this variety, and it is necessary to find a new breeding method to overcome the above-mentioned defects. Tissue culture is one option
At present, there is no information about the plant tissue culture of Buglossia cordata "Hades Ben ice cream" in China, and the field of plant tissue culture of Buglossia cordata "Hades Ben ice cream" is still blank

Method used

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  • Tissue culture method of brunnera macrophylla "Hadspen Cream"
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  • Tissue culture method of brunnera macrophylla "Hadspen Cream"

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] The present embodiment provides a tissue culture method of Buglossia cordata "Hades Ben ice cream", specifically comprising the following steps:

[0064] 1. Acquisition and Sterilization of Explants

[0065] (1) Pre-cultivation of the mother plant: place the mother plant in a greenhouse for 30-60 days, remove dead leaves and diseased leaves in time during the growth process, and spray carbendazim once every 15 days;

[0066] (2) Acquisition of explants: select the mother plant that grows robustly in the above-mentioned pre-cultivation, has good traits and no damage by diseases and insect pests, takes the inflorescence on the mother plant that has a growth period of 20 days, removes all the florets, and obtains the inflorescence axis;

[0067] (3) Disinfection of explants:

[0068] A. Rinse the acquired inflorescence axis under flowing tap water for 10 minutes and dry;

[0069] B. Cut the inflorescence rachis treated in step A into 2 cm inflorescence rachis, sterilize ...

Embodiment 2

[0081] This embodiment provides a tissue culture method of Buglossia cordata "Hadesben ice cream". The difference between this embodiment and Example 1 is the following points:

[0082] 1. During the acquisition and disinfection steps of the explants:

[0083] Step (2) in the acquisition of explants: select the inflorescence whose growth period of the mother plant is 25 days, remove all the florets, and obtain the inflorescence axis; step (3) in the disinfection of the explants:

[0084] A. Rinse the acquired inflorescence axis under flowing tap water for 10 minutes and dry;

[0085] B. Cut the inflorescence rachis treated in step A into small inflorescence rachis of 3 cm, sterilize in 75% alcohol for 30 seconds, shake gently, and be careful not to break the inflorescence rachis; then wash twice with heavy distilled water, shaking each time 3min;

[0086] C. Soak the inflorescence axis treated in step B in 0.1% mercuric chloride solution for 7 minutes; then wash it 3 times w...

Embodiment 3

[0098] This embodiment provides a tissue culture method of Buglossia cordata "Hadesben ice cream", and the difference between this embodiment and Example 2 lies in the following points:

[0099] 1. During the acquisition and disinfection steps of the explants:

[0100] Step (2) during the acquisition of explants: select the inflorescence whose growth period of the mother plant is 30 days, remove all the florets, and obtain the inflorescence axis;

[0101] In the disinfection of step (3) explant:

[0102] A. Rinse the acquired inflorescence axis under flowing tap water for 10 minutes and dry;

[0103] B. Cut the inflorescence rachis treated in step A into small inflorescence rachis of 3 cm, sterilize in 75% alcohol for 30 seconds, shake gently, and be careful not to break the inflorescence rachis; then wash twice with heavy distilled water, shaking each time 3min;

[0104] C. Soak the inflorescence axis treated in step B in 0.1% mercuric chloride solution for 9 minutes; then...

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Abstract

The invention relates to the technical field of plant in-vitro culture, in particular to a tissue culture method of a brunnera macrophylla "Hadspen Cream". The tissue culture method comprises the following steps of obtaining and disinfecting explants, inducing the explants to form calluses, inducing the calluses to form adventitious buds, proliferating the adventitious buds and inducing the adventitious buds to root; and in the step of obtaining and disinfecting the explant, a rachis of a mother plant which grows strongly and is free of plant diseases and insect pests is selected as the explant, disinfection treatment is conducted on the rachis, and the disinfected explant is obtained. According to the tissue culture method of the brunnera macrophylla "Hadspen Cream", the propagation efficiency of the brunnera macrophylla "Hadspen Cream" can be improved, meanwhile, the limitation of seasons on the production of the variety is reduced, so that the application of the brunnera macrophylla"Hadspen Cream" is promoted.

Description

technical field [0001] The present application relates to the technical field of plant in vitro culture, in particular to a tissue culture method of Buglossia cordata "Hadesben ice cream". Background technique [0002] Brunnera macrophylla "Hadspen Cream" (Brunnera macrophylla'Hadspen Cream'), a plant of the genus Buglossia in the Boraginaceae family, is a perennial herb that tolerates half shade and grows well under shaded conditions such as under trees, avoiding strong light Long-term direct sunlight, likes loose and well-drained sandy loam. The plant is clustered, hemispherical, and naturally unfolded; the leaves are green oval-shaped heart-shaped, with entire margins and light yellow leaf margins; the leaves and leaf backs are densely covered with white pilose, and the inflorescence rachis, pedicels and sepals are densely covered with striae Plumber, flowering period is March-May. Sexually likes cool and humid climate, not resistant to high temperature, and enters dorm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 林巧玲张学超辛培培杨聪儿董志男李现坤
Owner BEIJING GREEN GARDEN GRP CO LTD
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