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Screening method of febrile convulsion model rat and application thereof

A screening method and model technology, which is applied in the fields of pharmaceutical formulations, preparations for in vivo experiments, animal husbandry, etc., can solve the problems of not being able to become an animal model, the mortality rate of body surface burns, and the low success rate of convulsions, and achieve the goal of model induction. The effect of high success rate, maintenance of genetic predisposition, and short induction time

Inactive Publication Date: 2012-07-04
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the direct heating method of the body surface will cause the body surface of the experimental mice to be scalded and the mortality rate is high (Holtzman, D., Obana, K., and Olson, J.Hyperthermia-induced seizures in therat pup: A model for febrile convulsions.Science .1981, 213(4511): 1034-1036.)
The success rate of lipopolysaccharide-induced convulsions is low, only 50%, and the influence of foreign substances on model construction cannot be ruled out, so it cannot be a suitable animal model (Heida JG, Boisse L, Lipopolysaccharide-induced febrile convulsions in the rat: short-term sequelae. Epilepsia. 2004, 45(11): 1317-1329.)

Method used

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  • Screening method of febrile convulsion model rat and application thereof
  • Screening method of febrile convulsion model rat and application thereof
  • Screening method of febrile convulsion model rat and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] A screening method for febrile convulsion sensitive model mice, the steps are as follows:

[0055] (1) In the initial screening experiment, 100 (half male and half male) 21-day-old normal rats with SPF grade were selected. Put the rat into a transparent glass cylinder (diameter 6cm, height 20cm), and the lower part of the glass cylinder has a plurality of small holes communicating with the outside. The cylinder was erected in a constant temperature water bath, and the depth of the water in the cylinder was adjusted by placing a plastic gasket on the bottom of the glass cylinder, so that only the head could be exposed when the rat stood along the wall of the cylinder. The temperature for the first screening is 43.0°C, the temperature for the second screening is 44.0°C, and the temperature for the third screening is 45.0°C. After each water bath for 4 minutes, the rats were taken out for observation. According to the 5-level scoring standard of febrile seizures, grade 3...

Embodiment 2

[0060] A screening method for febrile convulsion tolerance model mice, the steps are as follows:

[0061] (1) In the initial screening experiment, 100 (half male and half male) 21-day-old normal rats with SPF grade were selected. Put the rat into a transparent glass cylinder (diameter 6cm, height 20cm), and the lower part of the glass cylinder has a plurality of small holes communicating with the outside. The cylinder was erected in a constant temperature water bath, and the depth of the water in the cylinder was adjusted by placing a plastic gasket on the bottom of the glass cylinder, so that only the head could be exposed when the rat stood along the wall of the cylinder. The temperature for the first screening is 43.0°C, the temperature for the second screening is 44.0°C, and the temperature for the third screening is 45.0°C. After each water bath for 4 minutes, the rats were taken out for observation, and screened according to the 5-grade scoring standard of febrile convu...

Embodiment 3

[0065] Example 3: Gene chip expression profiling analysis of febrile convulsion sensitive and tolerant model mice

[0066] Using the information provided by the gene expression profile chip to conduct high-throughput information analysis, obtain gene functions, signaling pathways, and metabolic pathways involved in differentially expressed genes related to febrile convulsions, so as to gain a deeper understanding of the pathogenesis of febrile convulsions. The clustering heatmap is a hierarchical clustering of the samples and genes, and then it is drawn together with the heatmap. The expression of the same gene in different samples can be observed through the heat map, and the grouping of samples can also be verified according to the gene expression.

[0067] The experimental steps are as follows:

[0068] 1. Animal screening: From the first generation of 100 (half male and half male) 21-day-old SPF rats, use the above method to screen generation by generation, and finally se...

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Abstract

The invention relates to the technical field of establishment of an animal model used for screening drug and researching disease mechanism, in particular to a screening method of a febrile convulsion model rat, which can be used for obtaining the model rat by screening in a way of combining a typical water bath heating induction model with artificial directional selection and mating breeding. Themethod is unique and can be used for successfully obtaining the good febrile convulsion sensitive model rat and a tolerance model rat.

Description

technical field [0001] The present invention relates to the technical field of establishment of animal models for drug screening and disease mechanism research, in particular to a screening method for febrile convulsion model mice, which is a method of manual directional selection and mating breeding through febrile convulsion models Screening method to obtain model mice. Background technique [0002] Febrile convulsions are the most common emergency symptoms of central nervous system dysfunction in childhood, especially in infants and young children, with a total incidence of about 2% to 4%, 2% to 5% in Europe and the United States, and 6% in Japan ~9%, and even as high as 14% in some areas. Febrile convulsions can lead to sequelae of the nervous system. Retrospective studies have shown that 40% of patients with a history of complex febrile convulsions will develop hippocampal sclerosis and temporal lobe epilepsy. At the same time, epidemiological investigations have shown...

Claims

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Application Information

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IPC IPC(8): A01K67/027A61K49/00
Inventor 何小华刘万红彭碧文尹君余方徐健李亮范元腾
Owner WUHAN UNIV
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