Screening method of febrile convulsion model rat and application thereof

A screening method and model technology, which is applied in the fields of pharmaceutical formulations, preparations for in vivo experiments, animal husbandry, etc., can solve the problems of not being able to become an animal model, the mortality rate of body surface burns, and the low success rate of convulsions, and achieve the goal of model induction. The effect of high success rate, maintenance of genetic predisposition, and short induction time

Inactive Publication Date: 2012-07-04
WUHAN UNIV
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Problems solved by technology

Among them, the direct heating method of the body surface will cause the body surface of the experimental mice to be scalded and the mortality rate is high (Holtzman, D., Obana, K., and Olson, J.Hyperthermia-induced seizures in therat pup: A model for febrile convulsions.Science .1981, 213(4511): 1034-1036.)
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  • Screening method of febrile convulsion model rat and application thereof
  • Screening method of febrile convulsion model rat and application thereof
  • Screening method of febrile convulsion model rat and application thereof

Examples

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Example Embodiment

[0053] Example 1:

[0054] A screening method for febrile seizure sensitive model mice, the steps are as follows:

[0055] (1) In the initial screening experiment, 100 (male and female) 21-day-old SPF normal rats were selected. Put the rat into a transparent glass cylinder (diameter 6cm, height 20cm), the lower part of the glass cylinder has a number of small holes communicating with the outside. Erect the cylinder in a constant temperature water bath, and adjust the depth of the water in the cylinder by placing a plastic gasket on the bottom of the glass cylinder, so that only the head can be exposed when the rat stands along the cylinder wall. The first screening temperature was 43.0°C, the second screening temperature was 44.0°C, and the third screening temperature was 45.0°C. The rats were taken out for observation after 4 minutes in each water bath. Screened according to the 5-level scoring standard of the seizure level of febrile seizures, level 3 or higher is sensitive, t...

Example Embodiment

[0059] Example 2:

[0060] A screening method for febrile seizure tolerance model mice, the steps are as follows:

[0061] (1) In the initial screening experiment, 100 (male and female) 21-day-old SPF normal rats were selected. Put the rat into a transparent glass cylinder (diameter 6cm, height 20cm), the lower part of the glass cylinder has a number of small holes communicating with the outside. Erect the cylinder in a constant temperature water bath, and adjust the depth of the water in the cylinder by placing a plastic gasket on the bottom of the glass cylinder. The rat can only expose his head when standing along the cylinder wall. The first screening temperature was 43.0°C, the second screening temperature was 44.0°C, and the third screening temperature was 45.0°C. The rats were taken out for observation after 4 minutes of each water bath, and they were screened according to the 5-level scoring standard of the febrile seizure seizure grade. Grade 0 or 1 is tolerance. The low...

Example Embodiment

[0065] Example 3: Analysis of gene chip expression profile of febrile seizure sensitive and tolerance model mice

[0066] Use the information provided by the gene expression profile chip to conduct high-throughput information analysis to obtain gene functions, signal pathways, and metabolic pathways involved in differentially expressed genes related to febrile seizures, so as to gain a deeper understanding of the pathogenesis of febrile seizures. The clustering heat map is to perform hierarchical clustering of samples and genes together with the heat map. The heat map can be used to observe the expression of the same gene in different samples, and to verify the grouping of samples according to the gene expression.

[0067] The experimental steps are as follows:

[0068] 1. Animal screening: from the first generation of 100 (male and female) 21-day-old SPF rats, the above-mentioned method was used to screen for generations, and finally the third generation rats were selected as the m...

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Abstract

The invention relates to the technical field of establishment of an animal model used for screening drug and researching disease mechanism, in particular to a screening method of a febrile convulsion model rat, which can be used for obtaining the model rat by screening in a way of combining a typical water bath heating induction model with artificial directional selection and mating breeding. Themethod is unique and can be used for successfully obtaining the good febrile convulsion sensitive model rat and a tolerance model rat.

Description

technical field [0001] The present invention relates to the technical field of establishment of animal models for drug screening and disease mechanism research, in particular to a screening method for febrile convulsion model mice, which is a method of manual directional selection and mating breeding through febrile convulsion models Screening method to obtain model mice. Background technique [0002] Febrile convulsions are the most common emergency symptoms of central nervous system dysfunction in childhood, especially in infants and young children, with a total incidence of about 2% to 4%, 2% to 5% in Europe and the United States, and 6% in Japan ~9%, and even as high as 14% in some areas. Febrile convulsions can lead to sequelae of the nervous system. Retrospective studies have shown that 40% of patients with a history of complex febrile convulsions will develop hippocampal sclerosis and temporal lobe epilepsy. At the same time, epidemiological investigations have shown...

Claims

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Application Information

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IPC IPC(8): A01K67/027A61K49/00
Inventor 何小华刘万红彭碧文尹君余方徐健李亮范元腾
Owner WUHAN UNIV
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