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Induction method for autotetraploids of pomegranates

An autotetraploid and pomegranate technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low induction success rate and cumbersome induction operation methods, and achieve high induction success rate and simple operation Effect

Inactive Publication Date: 2015-05-27
攀枝花市农林科学研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current induction operation method is cumbersome, and the induction success rate is not high

Method used

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  • Induction method for autotetraploids of pomegranates
  • Induction method for autotetraploids of pomegranates

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experiment example

[0032](1) In November 2013, at the Panzhihua Academy of Agriculture and Forestry Sciences, the self-bred seeds of 'Israel soft seed acid' pomegranate (the number of chromosomes is 2n=2x=16) were selected as test materials, and the seeds were washed and dried and placed in Treat at a low temperature of 4°C for 4 days, and then put them into a 27°C incubator to accelerate germination to the state of germination.

[0033] (2) Configure a 0.3% colchicine solution (adding 1% DMSO) mutagenic reagent, shading and soaking to germinate the seeds of 'Israeli soft seed acid', and place them on a constant temperature shaker at 25°C to oscillate at a speed of 100r / min Induced culture. The soaking time of colchicine solution was set to four treatments of 0h, 12h, 24h, and 36h, with 60 germinated seeds in each treatment, and the experiment was repeated 3 times.

[0034] (3) Soak and wash the seeds of 'Israeli soft seed acid' soaked in colchicine solution with tap water for 3-5 times, each s...

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Abstract

The invention provides an induction method for autotetraploids of pomegranates. The induction method comprises the following steps: (1) harvesting pomegranate fruits matured by selfing, taking seeds, cleaning with clean water, drying in the sun, treating the dried seeds in the condition with the temperature of 4 DEG C for 4 days, then putting into a calorstat with the temperature of 27 DEG C and germinating to be in a budding state; (2) soaking the budding pomegranate seeds obtained in the step (1) by using a colchicine solution with the mass-percentage concentration of 0.3%, simultaneously putting the pomegranate seeds on a sunshine-shading constant-temperature swinging bed with the temperature of 25 DEG C, carrying out oscillatory induction culture for 24 hours at speed of 100r / min, and using tap water to soak and clean for 3-5 times with 5 minutes for each time after treatment. The induction method provided by the invention has the advantages that the defect of tedious operation of the general ploidy-induction method is overcome, the operation is simple and easy, the success rate of induction is high, and the need for seed selection of polyploidy is met, so that the polyploidy breeding becomes an important method for genetic improvement and new-variety breeding of the pomegranates.

Description

technical field [0001] The invention relates to the technical field of plant germplasm resource innovation, in particular to a pomegranate autotetraploid induction method. Background technique [0002] Pomegranate is the earliest fruit tree cultivated in the world. There are more than 1,000 varieties. It is widely planted and distributed in tropical and subtropical regions of the world. It has high economic value, medicinal value and ornamental value. In recent years, many excellent pomegranate varieties have been bred through cross breeding, bud mutation selection, seed selection, and introduction and domestication of foreign varieties in China, and more than 280 types of pomegranate varieties have been preserved. According to research, the existing cultivated pomegranates are diploid, and the number of chromosomes in somatic cells has two types: 2n=2x=18 and 2n=2x=16. After polyploidization of plants, vegetative and reproductive organs are prone to enlargement, and seconda...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 潘宏兵李贵利张洪祥黄云刘斌杜邦
Owner 攀枝花市农林科学研究院
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