Culture method for enhancing functionality of hepatocytes and used hepatocyte culture solution
A technology of hepatocytes and culture medium, applied in cell culture active agent, culture process, animal cells and other directions, can solve the problems of difficulty in human primary hepatocytes, short in vitro proliferation cycle, etc. simple effect
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[0030] The present application will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0031] This application provides a hepatocyte culture medium, which is used to induce hepatocytes with a lower degree of differentiation, to enhance the specific function of hepatocytes, and to make the physiological state of induced hepatocytes closer to the physiological state of hepatocytes in the human body State, or close to the physiological state of human primary hepatocytes, to improve the possibility of clinical application.
[0032] The hepatic cell culture medium of the present application comprises the following components: dexamethasone with a final concentration of 1-20 μM, curcumin with a final concentration of 1-20 μM and basal medium.
[0033] The basal medium is selected from one of DMEM / F12 cell culture medium, F12 cell culture medium and William's E cell culture medium. DMEM / F-12 (Dulbecco's Modified Eagle Mediu...
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