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Construction method and application of a kind of epilepsy animal model

A technology of epileptic animals and construction methods, which is applied in the field of preparation of medical and biological experimental models, and can solve problems such as low epilepsy levels, large differences, and unfavorable scientific research progress

Active Publication Date: 2021-06-04
WUHAN UNIV
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Problems solved by technology

Therefore, MIC19 plays a crucial role in the assembly, stability and maintenance of the normal shape of the mitochondrial cristae of the MICOS complex, and has an important impact on the occurrence and change mechanisms of epilepsy; however, most of the existing epilepsy animal models use drugs Induction, drug induction has defects such as low induction success rate, low epilepsy grade, and large differences among individuals induced by the same batch, which is not conducive to the progress of related scientific research

Method used

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  • Construction method and application of a kind of epilepsy animal model
  • Construction method and application of a kind of epilepsy animal model
  • Construction method and application of a kind of epilepsy animal model

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[0030] For a better understanding of the present invention, the following examples are further descriptions of the present invention, but the content of the present invention is not limited to the following examples.

[0031] Such as figure 1 Shown is the basic flow chart of the epilepsy animal model construction method in this embodiment. In this embodiment, the gene sequence encoding FRT-PGK-neomycin-FRT-Loxp is first inserted into the wild-type Mic19 allele carrier using Cre-LoxP technology. The intron sequence between exon 2 and exon 3, and the intron sequence after the exon 1 is inserted into another loxp sequence in the same direction to construct a targeting vector. The gene sequence encoding FRT-PGK-neomycin-FRT-Loxp is a gene sequence with neomycin resistance; then, the targeting vector is microinjected into the embryonic stem cells of C57BL / 6 strain mice, and polymerized Enzyme chain reaction technology (Polymerase Chain Reaction, hereinafter referred to as PCR tech...

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Abstract

The invention relates to the technical field of preparation of medical and biological experimental models, in particular to a construction method and application of an epilepsy animal model. An animal model of spontaneous epilepsy was constructed by specifically knocking out Mic19 in animals. The epilepsy animal model obtained by adopting the construction method of the present invention has better stability, lower individual differences, and high induction success rate.

Description

technical field [0001] The invention relates to the technical field of preparation of medical and biological experimental models, in particular to a construction method and application of an epilepsy animal model. Background technique [0002] Epilepsy is abnormal synchronous firing of neurons, and its mechanism is mainly mediated by glutamate, aspartate and γ-aminobutyric acid. As a neuroexcitatory transmitter, glutamate (Glutamic acid, hereinafter referred to as Glu) can be absorbed by the Glu receptor on the post-synaptic membrane, that is, the N-methyl-D-aspartic acid receptor (N-methyl-D -aspartic acid receptor, hereinafter referred to as NMDAR), which causes calcium ion influx, changes the expression and activity of key molecules in the subsequent signal transduction pathway, and finally destroys the excitation-inhibition balance in the brain. [0003] Studies have found that mitochondrial dysfunction can cause calcium ion dynamic imbalance in neurons, leading to calc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/85C12N15/90A01K67/027
CPCA01K67/0275A01K2217/075A01K2227/105A01K2267/03C12N15/85C12N15/907
Inventor 宋质银左梅灵
Owner WUHAN UNIV
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