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Method for constructing epilepsy animal model and application

A technology for epilepsy animals and construction methods, which is used in the preparation of medical and biological experimental models, and can solve the problems of low induction success rate, low epilepsy grade, and large differences.

Active Publication Date: 2020-04-03
WUHAN UNIV
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Problems solved by technology

Therefore, MIC19 plays a crucial role in the assembly, stability and maintenance of the normal shape of the mitochondrial cristae of the MICOS complex, and has an important impact on the occurrence and change mechanisms of epilepsy; however, most of the existing epilepsy animal models use drugs Induction, drug induction has defects such as low induction success rate, low epilepsy grade, and large differences among individuals induced by the same batch, which is not conducive to the progress of related scientific research

Method used

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  • Method for constructing epilepsy animal model and application
  • Method for constructing epilepsy animal model and application
  • Method for constructing epilepsy animal model and application

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Embodiment Construction

[0030] For a better understanding of the present invention, the following examples are further descriptions of the present invention, but the content of the present invention is not limited to the following examples.

[0031] Such as figure 1 Shown is the basic flow chart of the epilepsy animal model construction method in this embodiment. First, the Cre-LoxP technology is used to insert the gene sequence encoding FRT-PGK-neomycin-FRT-Loxp into the wild-type Mic19 allele carrier The intron sequence between exon 2 and exon 3, and the intron sequence after the exon 1 is inserted into another loxp sequence in the same direction to construct a targeting vector. The gene sequence encoding FRT-PGK-neomycin-FRT-Loxp is a gene sequence with neomycin resistance; then, the targeting vector is microinjected into the embryonic stem cells of C57BL / 6 strain mice, and polymerized Enzyme chain reaction technology (Polymerase Chain Reaction, hereinafter referred to as PCR technology) screens ...

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Abstract

The invention relates to the technical field of preparation of medical and biological experiment models and in particular relates to a method for constructing an epilepsy animal model and application.By a specific animal Micl9 knockout mode, an animal model of automatic epilepsy is constructed. The epilepsy animal model constructed by using the method provided by the invention has good stability,low individual differences and high induction success rates.

Description

technical field [0001] The invention relates to the technical field of preparation of medical and biological experimental models, in particular to a construction method and application of an epilepsy animal model. Background technique [0002] Epilepsy is abnormal synchronous firing of neurons, and its mechanism is mainly mediated by glutamate, aspartate and γ-aminobutyric acid. As a neuroexcitatory transmitter, glutamate (Glutamic acid, hereinafter referred to as Glu) can be absorbed by the Glu receptor on the post-synaptic membrane, that is, the N-methyl-D-aspartic acid receptor (N-methyl-D -aspartic acid receptor, hereinafter referred to as NMDAR), which causes calcium ion influx, changes the expression and activity of key molecules in the subsequent signal transduction pathway, and finally destroys the excitation-inhibition balance in the brain. [0003] Studies have found that mitochondrial dysfunction can cause calcium ion dynamic imbalance in neurons, leading to calc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85C12N15/90A01K67/027
CPCA01K67/0275A01K2217/075A01K2227/105A01K2267/03C12N15/85C12N15/907
Inventor 宋质银左梅灵
Owner WUHAN UNIV
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