Pyrus pyrifolia polyploid induction method

A polyploid, sand pear technology, applied in the field of plant tissue culture, can solve problems such as difficult to cultivate successfully, and achieve the effects of low cost, good induction orientation and simple operation

Inactive Publication Date: 2012-11-07
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

If you choose poorly, it will be difficult to achieve success in cultivation

Method used

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  • Pyrus pyrifolia polyploid induction method
  • Pyrus pyrifolia polyploid induction method

Examples

Experimental program
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Embodiment 1

[0031] (1) Take the sand pear variety 'Cuiguan' tissue culture seedlings cultivated in the tissue culture laboratory of the Pear Engineering Technology Research Center of Nanjing Agricultural University on the ultra-clean workbench. into single-bud stem segments with leaves;

[0032] (2) Under the condition of shading, soak the single-bud stem section of 'Cuiguan' in the autoclaved 0.1% colchicine solution, and then shake it on the shaker at 130r / min for 48 hours. After the treatment, no Rinse with bacterial water 3 times;

[0033] (3) Transfer the dipped single-bud stem section to the proliferation medium containing 0.003wt% colchicine, and the axillary buds of the single-bud stem section should be buried in the proliferation medium so that the growth point is fully in contact with the medicinal solution , the leaves are exposed to the culture medium, and after being cultured in the dark for 6 days, they are transferred to culture under light, and the axillary buds to be tre...

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Abstract

The invention relates to a Pyrus pyrifolia polyploid induction method. The Pyrus pyrifolia polyploid induction method includes subjecting a single-bud stem segment of a Pyrus pyrifolia tissue culture seedling to polyploid induction with colchicine, performing differentiation culture and rooting culture after induction, and performing polyploid identification. The Pyrus pyrifolia polyploid induction method has the advantages of high induction directionality, low cost and simplicity in operation. The Pyrus pyrifolia polyploid induction method is a novel feasible efficient method for Pyrus pyrifolia polyploid breeding, and has significant application value in promoting artificial induction techniques for Pyrus pyrifolia polyploid in China.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a method for inducing polyploidy of pear. Background technique [0002] Pear is a traditional popular fruit in my country. It is rich in nutrition and has a good taste. It is cold in nature, clears away heat and moistens the lungs, and has high edible value and medical value. As an important woody economic fruit tree, pear has wide adaptability to climate and soil, and strong stress resistance. It is planted in various provinces and regions in my country (except Hainan Province). In plant taxonomy, pear is a plant of the genus Pyrus of the subfamily Maloldeae of the Rosaceae (Rosaeeae), and is a deciduous fruit tree of the Arbor. According to the current classification, there are 60 species of pears in the world, and 16 species originate in China. In terms of production and cultivation, pears can be divided into two categories: Oriental pears and Western pears. Most...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张绍铃薛亚男吴俊陶书田齐开杰
Owner NANJING AGRICULTURAL UNIVERSITY
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