Method for culturing in vitro tissue of populuseuphratica

Abstract

The invention provides a method for culturing in vitro tissue of populuseuphratica. The method comprises steps as follows: a stem provided with a bud of the populuseuphratica is inoculated to an initiation culture medium for initiation culture so as to obtain an axillary bud of the populuseuphratica; the axillary bud of the populuseuphratica is transferred to a subculture medium for subculture so as to obtain a populuseuphratica seedling; and the populuseuphratica seedling is transferred to a rooting medium for rooting culture so as to obtain a tissue culture seedling of the populuseuphratica. With adoption of the method, the vitro tissue of the populuseuphratica can be cultured effectively, the induction success rate and the final rooting rate are quite high, so that a large quantity of tissue culture seedlings of the populuseuphratica can be obtained in a short time, and the method is simple and convenient to operate and is suitable for large-scale and fast populuseuphratica reproduction.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a method for cultivating isolated tissues of Populus euphratica. Background technique [0002] Populus euphratica Oliv is a plant of the genus Populus in the family Salicaceae. It is distributed in Mongolia, Central Asia, Egypt, Syria, India and other places. In my country, it is mainly distributed in western Inner Mongolia, Gansu, Qinghai, Xinjiang and other places. . Populus euphratica is an arbor, 10-15 meters high, thin shrub-like, with variable leaf shapes, oval, oval-lanceolate, triangular-shaped oval or kidney-shaped, so it is also named Populus heterophyllum. Under certain conditions, the life span can reach about a hundred years. Populus euphratica is a tree species that grows under arid continental climate conditions. It is well known for its excellent drought and salinity resistance. Its wood can be used for construction, bridges, agricultural tools, furniture, etc., ...

AI Technical Summary

Problems solved by technology

[0003] The genetic background of Populus euphratica is complex, and its propagation method is mainly seed propagation, but the use of seed propagation shows the instability of genetic characters in the process of forestry production and scientific research, and this propagation method has the problem of serious separation of seedlings, and the growth status is uneven , and the long-term inbreeding caused the degradation of Populus euphratica species and made its growth status worse

And in the cloning process of Populus euphratica, there is a characteristic that the cuttings are not easy to survive

Utilizing tissue culture and other asexual reproduction techniques can not only establish the clones of Populus euphratica, but also accelerate the breeding speed of Populus euphratica seedlings. However, yellowing and vitrification generally occur during the tissue culture process, and the asexual reproduction technology is still immature

[0004] Therefore, the breeding technology of Populus euphratica still needs to be improved

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