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Method for culturing autotetraploid paris polyphylla plant

A technology of aesculus and one flower, allotetraploid, applied in the directions of botanical equipment and methods, horticultural methods, plant regeneration, etc., can solve problems such as degradation, and achieve expansion of planting area, high induction success rate, and increase of planting income. Effect

Inactive Publication Date: 2014-11-26
华颂种业(北京)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can quickly realize the cultivation of autotetraploid Echinacea esculenta plants, thereby effectively increasing the content of its active ingredients, and overcoming the variety degradation problem of Aesculus aesculus in natural reproduction and artificial planting

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 The cultivation method of autotetraploid Aesculus aesculinum plant

[0026] Proceed as follows:

[0027] (1) Breeding tissue cultured seedlings: get the leaf buds of Aesculus aesculinum plant, and breed tissue cultured seedlings by plant tissue culture technology;

[0028] (2) Colchicine treatment: Under aseptic conditions, select tissue-cultured subcultured seedlings with strong growth potential, cause several (2-5) wounds on the stem, then wrap them tightly with absorbent cotton, and then drop them into the absorbent cotton. The mass concentration is 0.05% colchicine solution infiltration, cultured on the differentiation medium for 12 days, washed with sterile water, cut into single buds and then transferred to cluster bud induction medium;

[0029] The components of the differentiation medium consist of: MS+BA 0.6mg / L+GA 3 0.3mg / L+NAA 0.2mg / L+CTK0.4mg / L;

[0030] The culture conditions for culturing in the differentiation medium are: temperature 28°C...

Embodiment 2

[0038] Embodiment 2 The cultivation method of autotetraploid Aesculus aesculinum plant

[0039] The steps are as follows: (1) Breeding tissue cultured seedlings: take the leaf buds of the Aesculus aesculata plant, and breed the tissue cultured seedlings through the plant tissue culture technique;

[0040] (2) Colchicine treatment: transfer the tissue-cultured seedlings to a differentiation medium with a mass concentration of 0.08% colchicine, and after culturing for 25 days, cut into single buds and transfer them to clustered bud induction medium;

[0041] The components of the differentiation medium consist of: MS+BA 0.6mg / L+GA 3 0.3mg / L+NAA 0.2mg / L+CTK0.4mg / L;

[0042] The culture conditions for culturing in the differentiation medium are: temperature 28°C, sunshine 10h per day, light intensity 60μmol·m -2 ·s -1 ;

[0043] The components of the cluster bud induction medium consist of: MS+BA 1.8mg / L+GA 3 0.3mg / L+NAA 0.3mg / L+sucrose 25g / L;

[0044] (3) Inducing plant d...

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Abstract

The invention discloses a method for culturing an autotetraploid paris polyphylla plant, which solves the problem of variety degeneration in natural propagation and artificial planting of paris polyphylla. The method comprises the steps of (1) cultivating tissue culture seedlings; (2) performing colchicines treating, namely performing treatment by one of the following two methods: firstly, selecting a tissue subculture seedling under a germfree condition, forming a plurality of wounds at the stem part, closely covering the tissue subculture seedling by degreasing cotton, dropping a colchicines solution on the degreasing cotton for soaking, performing culture on a differentiation culture medium, cleaning the tissue subculture seedling by germfree water, cutting the tissue subculture seedling into single buds, and transferring the single buds into a rooting induction culture medium; and secondly, transferring the tissue culture seedling into the differentiation culture medium added with colchicines, after culture is performed, cutting the tissue culture seedling into single buds, and transferring the single buds into a cluster bud induction culture medium; (3) performing differentiation culture on the plant; (4) performing multiplication culture on the plant; (5) detecting chromosomes of the plant; and (6) selecting and culturing the autotetraploid plant. The method disclosed by the invention has a great significance on enlarging of the planting area of peculiar valuable medicinal plants.

Description

technical field [0001] The invention relates to a method for cultivating an autotetraploid Echinacea esculenta plant, belonging to the field of medicinal plant breeding. Background technique [0002] Aescin is a perennial herbaceous plant of the genus Pachyphylla in the family Liliaceae. It grows in the forest at an altitude of 1800-3200 meters in the wild, in the shade of the hillside forest or in the damp place by the ditch. It is distributed in Yunnan, Sichuan, Guizhou, China. Guangdong, Guangxi, Jiangxi, Fujian and other provinces. The dried rhizome is used as medicine, and the traditional Chinese medicine names are Chonglou, Aescin, and Dengtaiqi. The rhizome contains various steroidal saponins, alkaloids and amino acids. Traditional Chinese medicine believes that its nature and taste are bitter and slightly cold, and it has the effects of clearing heat and detoxification, reducing swelling and pain, calming wind and convulsions, relieving asthma and relieving cough, ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王晓娟
Owner 华颂种业(北京)股份有限公司
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