41results about How to "Slow down proliferation" patented technology

The invention discloses a production method of a high-salt diluted and low-temperature fragrant soy sauce, which comprises the following steps of: (1) curing raw materials including wheat and defatted soybeans, and then uniformly mixing the wheat and the defatted soybeans; (2) inoculating aspergillus oryzae strains to the obtained raw material mixture so as to obtain a yeast; (3) cultivating the yeast for 44-48 hours at a temperature of 25-30 DEG C so as to obtain a mature yeast; (4) adding 2.0-2.5 weight times of saline water into the mature yeast so as to obtain a raw soy sauce mash; (5) moving the raw soy sauce mash to the inside of an airtight fermentation tank for fermenting: firstly fermenting for 23-27 days at a temperature of 13-17 DEG C, then continuing to ferment at a temperature of 28-32 DEG C, and on the 28-32nd days, adding mixed fermentation strains into the fermentation tank and continuing to ferment; in the process of fermentation, stirring by using a compressed air flow; and fermenting for more than six months so as to obtain a mature soy sauce mash; and (6) squeezing and filtering the mature soy sauce mash so as to obtain the low-temperature fragrant soy sauce. According to the invention, the conversion rate of proteins in raw materials is high, and the contents of ammonia nitrogen, total nitrogen, soybean peptides and soybean oligosaccharides in the prepared soy sauce are high.

The invention discloses application of a reagent for inhibiting circ_CLASP2 (Cytoplasmic Linker Associated Protein 2) in preparation of a preparation for treating nasopharyngeal darcinoma and the preparation. A research proves that the circ_CLASP2 is inhibited in nasopharyngeal darcinoma cells, and the proliferation, invasion and metastasis of the nasopharyngeal darcinoma cells can be inhibited. Therefore, a circ_CLASP2 inhibitor is used for treating the nasopharyngeal darcinoma and has profound clinical significance and an important popularization and application prospect.

The invention relates to a compound extracted from drug fungus and natural traditional medicine, which can adjust the balance of enterocin, restrain cancer, and improve healthy. It comprises friend germ, ramalic polysaccharide and polygonum multiflorum thumb polysaccharide, while their contents are 10<8cfu-3*10<8cfu / g, 1g-3g, and 1g-3g.

The invention relates to a compound fresh-preserving agent, which is prepared by the following raw materials in proportion by weight: 5-20 nisin from streptococcus lactis, 10-60 sodium dehydroacetate, 5-50 Glucono- beta-lactone, 5-40 Sodium Erythorbate, 5-50 sodium chloride, and 1-50 sodium tripolyphosphate. The compound fresh-preserving agent of the invention uses the synergistic effect of various raw materials to enable the fresh-preserving effect to be obvious and stabilize sense, chemistry and microorganism indexes of meat and meat products, thus prolonging the shelf life of the meat and the meat products; and the invention has the advantages of wide application product range, convenient processing and use, safety and no toxicity.

The invention provides ringlike RNA circ-CCNY and application thereof. The nucleotide sequence of the circ-CCNY gene is as shown in SEQ ID NO: 1; the expression level of the ringlike RNA circ-CCNY is obviously reduced by detecting the expression condition of the circ-CCNY gene in a liver cancer patient; and the multiplication rate of the ringlike RNA circ-CCNY is obviously reduced by comparing liver cancer cells for expressing the RNA circ-CCNY gene with control liver cancer cells. The ringlike RNA circ-CCNY gene and an expression product thereof serve as markers of diagnosing liver cancer, so that diagnosis of the liver cancer is more accurate and quicker, and the ringlike RNA circ-CCNY gene as a target gene for preparing drugs capable of curing the liver cancer provides a new therapeutic target and therapy pathway for curing the liver cancer.

The invention discloses an antineoplastic medicine prepared through gelsmium elegans medicinal materials. The antineoplastic medicine comprises the following steps that fresh gelsmium elegans rattan stems and / or roots and / or leave and / or dry medicinal slices are taken, steeping and extracting are conducted, ethyl alcohol is recycled by reducing pressure, concentration is conducted until the alcohol taste is eliminated, acid is added for dissolving, PH is adjusted, and upper clear liquid is taken; alkali is added, PH is adjusted to be 9-10, solution extraction is conducted, organic solvent is recycled, and gelsmium elegans total alkaloid extractum is obtained; the extractum is dissolved through methyl alcohol and the like and blended with silica gel, drying and crushing are conducted, the upper silica gel is prepared to a column, gradient elute is conducted by organic solvent, and rough-wrought portions of sempervirine are obtained; and high-middle-pressure phase reversal silica gel column preparation is conducted. The gelsmium elegans total alkaloid begins to exert influences on activity of tumour cells after being added for four hours, and the multiplication speed of the tumour cells is lowered a little compared with a blank group; and the sempervirine begins to exert influences on activity of the tumour cells after being added for five hours, the sempervirine with a low dosage can lower the multiplication speed of the tumour cells, and the sempervirine with a high dosage can kill the tumour cells.

The invention provides ring-shaped RNA circ-NFATC3 and application thereof. The nucleotide sequence of a ring-shaped RNA circ-NFATC3 gene is as shown as SEQ ID NO:1. Besides, the invention provides a medicine composition, a liver cancer diagnosis kit and application of the circ-NFATC3 gene serving as a target gene to preparing medicine for treating liver cancer. By means of the circ-NFATC3 gene and expression products of the circ-NFATC3 gene as liver cancer diagnosis markers, liver cancer diagnosis is more accurate and quicker, and a new therapeutic target and a new treatment means are provided for liver cancer treatment by means of the circ-NFATC3 gene as the target gene for preparing medicine for treating liver cancer.

The invention discloses a wheat planting method resistant to soil pollution, and belongs to the field of planting. The planting method specifically comprises the following steps: (1) seed selection, (2) seed soaking liquid preparation, (3) seed treatment, and (4) germination seeding. According to the planting method, seed characteristics can be well improved, the vitality of the seeds is enhanced,the vitality of roots of later seedlings can be improved, and the absorption characteristics are improved, so that the absorption properties of the cultivated wheat plants on zinc, chromium, mercury,lead and nickel heavy metals are well improved, the stress resistance of the plants is improved, and the wheat yield is also improved; and in addition, the method is simple to handle, is easy to implement, and has high operability, therefore, the method has a very good promotion and use value.

The invention provides microRNA used for inducing leukemia cell differentiation and application thereof in the induction of erythroid differentiation of tumor cell lines K562. The microRNA not only has the advantages of cheapness, no toxic or side effect, long duration, high transfection efficiency and the like, but also has the characteristics of stronger action and effectiveness of various indexes for tumor cell differentiation compared with a conventional human leukemia cell erythroid differentiation siRNA inducer.

The invention discloses a premix capable of reducing propagation and invasion of Salmonella Enteritidis phage type 4 in a laying hen. The premix comprises, by mass, ferrous fumarate, ferrous lactate, zinc methionine chelate, zinc propionate, manganese methionine chelate, copper sulphate, cobalt acetate, a sodium selenite premix, a potassium iodide premix, magnesium sulfate and calcium carbonate. The invention also discloses a preparation method for the premix capable of reducing propagation and invasion of Salmonella Enteritidis phage type 4 in the laying hen. The additive premix can provide trace element nutritional components needed by the laying hen and reduce the positive rate of almonella Enteritidis phage type 4 in laying hens of common chicken flocks; and the proportion of salmonella-positive eggs is reduced by 20 to 30%, and the course that the excrement of the laying hen carries Salmonella Enteritidis phage type 4 is shorted by 7 to 14 d.

The invention discloses application of a substance for reducing USP1 expression in preparation of a medicine for treating children T-series acute lymphocytic leukemia. The invention discloses short hairpin RNA (Ribonucleic Acid) for forming a stem-loop structure. The short hairpin RNA is shRNA-1 or shRNA-2. The deubiquitination enzyme USP1 has the effects of inhibiting cell apoptosis and promotingcell proliferation in T-series acute lymphocytic leukemia cells, and the invention proves that inhibition of USP1 expression can promote apoptosis of tumor leukemia cells and inhibit proliferation ofleukemia cells. The invention provides a novel way for the treatment of T-series acute lymphocytic leukemia, and the deubiquitinase USP1 is expected to become a potential target for anti-leukemia treatment and has a very wide application prospect in the medical field.

The invention relates to a human primary tumor cell separation preparation method which comprises the following steps of washing tumor cells which are separated out by using a 0.9 percent sodium chloride injection, removing blood, and shearing necrotic and paeoniae tissues; shearing the tumor tissues into 1mm<3>, placing the shorn tumor tissues in a centrifugal tube, adding type I collagenase in the centrifugal tube, carrying out cold digestion overnight, adding hyaluronidase and DNA enzyme next day, carrying out digestion, sieving a mixed liquid after digestion by a cell screen, collecting a filtrate, and carrying out centrifugation to remove supernatant to obtain a single-cell suspension; adding a serum-free primary culture medium, beating, uniformly mixing, counting cells, inoculating the cells into a culture bottle, supplementing the serum-free primary culture medium, and placing the culture bottle in a carbon dioxide constant-temperature and constant-humidity incubator to carry out culture; and carrying out cryopreservation. The human primary tumor cell separation preparation method provided by the invention is high in harvest rate of prepared cells, enables the cells to be rapid in proliferation, can improve the proliferation capability of human primary tumor cells, can increase the proliferation speed of the human primary tumor cells, reduces the culture period and has prominent progress.

The invention relates to a ripening and degenerating treatment and utilization method of alive enteromorpha prolifera in offshore sea areas, and belongs to the technical field of environmental protection and biological processing. According to the method, before the enteromorpha prolifera enters near shores, the enteromorpha prolifera floating on the sea surface are collected, pulped, ripened anddegenerated into semi-fluid algin, and the semi-fluid algin is directly discharged into the sea areas through a delivery pipe; the semi-fluid algin serves as a good bait for various marine organisms to achieve resource utilization. By the method, offshore on-site treatment of the enteromorpha prolifera can be realized, and food sources convenient to absorb and utilize can be provided for the marine organisms; gametes of the enteromorpha prolifera can be efficiently killed due to the thermotropy, the reproductive effect of the enteromorpha prolifera can be efficiently inhibited, the proliferation speed of the enteromorpha prolifera is decreased when the enteromorpha prolifera drifts near the shores, propagation control and limited expansion of the enteromorpha prolifera in the offshore seaareas are benefited, and good economic, social and ecological benefits are achieved.

The invention discloses a stem cell cryopreservation liquid as well as a preparation method and a use method thereof. The stem cell cryopreservation liquid is prepared from 0 to 20 percent of DMSO, 1to 2 percent of glycerol, 1 to 2 percent of trehalose, 2 to 5 percent of ethanol, 0.05 to 0.07 percent of N-dodecanoyl sarcosine, 0.7 to 1.0 percent of calcium phosphate, 0.03 to 0.06 percent of polyporus acid C, 0.02 to 0.05 percent of trigonelline, 0.01 to 0.04 percent of 1, 8-cineole, and the balance of DMEM culture medium. After the stem cell cryopreservation liquid is used for cryopreservation, the stem cell resuscitation rate reaches 90% or above, and compared with non-cryopreserved cells, the proliferation speed is not obviously reduced and even increased.

The invention provides a purifying method for a sewer of a caravan. The purifying method comprises the following steps: 1) allowing sunlight to pass through a Fresnel lens, focus on a light collecting block and to be transmitted to a main optical fiber, then allowing the sunlight to pass through the main optical fiber and transmitting the sunlight to a scattering head of a photosynthetic bacterium culture cage via the main optical fiber, wherein light emitted by the scattering head provides a light source for the photosynthetic bacterium culture cage; (2) allowing green sulphur bacteria, red sulphur bacteria and Rhodospira bacteria suspended in a nutrient solution in the bacterium culture cage to be propagate under irradiation of sunlight, wherein the green sulphur bacteria, red sulphur bacteria and Rhodospira bacteria can mature in 7 days; and (3) closing an outlet valve of the sewer, opening a butterfly valve of an output pipe and allowing the green sulphur bacteria, red sulphur bacteria and Rhodospira bacteria cultured in the step 2 to flow into the sewer. The method can prevent microbial corrosion to a pipeline by harmful bacteria, realizes purification of an environment in the sewer and prolongs the service life of the pipeline of the sewer.

The invention discloses a traditional Chinese medicine compound composition for treating cancer. The technical essential is as follows: the traditional Chinese medicine compound composition comprises the following raw materials in parts by weight: 1.6-7 parts of chitosan oligosaccharide, 3-10 parts of red ginseng, 1-8 parts of trogopterus dung, 3-10 parts of pseudo-ginseng, 0.1-0.5 part of ganoderma lucidum spore, 0.1-0.5 part of grape seeds, 1-8 parts of amber, 3-10 parts of placenta and 0.5-5 parts of prepared pangolin scales. The combination is definite in curative effect, low in side effects, and suitable for traditional Chinese medicine oral medicines for treating cancer, bacterial canker and chronic diseases.

The invention belongs to the technical field of tumor molecular biology, and particularly relates to application of circ_2157 in preparation of a therapeutic preparation for nasopharyngeal carcinoma and the therapeutic preparation. Since siRNA has a good silencing effect, an MTT assay is performed in nasopharyngeal carcinoma cell lines HONE1, HNE2 and CNE2 in which the circ_2157 is silenced afterthe circ_2157 is disturbed, and the proliferation rate of cells in the siRNA group is significantly lower than that in the NC group, that is, the silenced circ_2157 inhibits the proliferation of nasopharyngeal carcinoma cells. That is, inhibition of the circ_2157 can treat the nasopharyngeal carcinoma, and the circ_2157 has far-reaching clinical significance and important application prospects.

The invention provides a perishable garbage containing device. The perishable garbage containing device comprises a box body, the box body is shaped like a square barrel, and the upper portion of thebox body is provided with a perishable garbage putting port; a first fence, a second fence, a stainless steel filter screen and a water accumulating basin are sequentially arranged in the box body from top to bottom; the first fence comprises a first fixing rod and multiple first straight rods arranged in parallel, and one end of each first straight rod is fixed to the first fixing rod; the secondfence comprises a second fixing rod and multiple second straight rods arranged in parallel, and one end of each second straight rod is fixed to the second fixing rod; the first fixing rod and the second fixing rod are both horizontally arranged and installed on the two inner walls with the opposite directions in the box body, the first fixing rod and the second fixing rod are both hinged to the corresponding inner walls in the box body, and the first straight rods and the second straight rods can rotate in the box body by taking the first fixing rod and the second fixing rod as shafts respectively; and limiting supporting blocks are arranged under the first fixing rod and the second fixing rod, and therefore the rotating angles of the first straight rods and the second straight rods underthe horizontal line do not exceed 15 degrees.

The invention belongs to the technical field of tumor molecular biology, and specifically relates to the application of circ_2157 in the preparation of nasopharyngeal carcinoma treatment preparations and the treatment preparations. Because siRNA has a good silencing effect, after ensuring that circ_2157 is interfered, the present invention conducts MTT experiments in the nasopharyngeal carcinoma cell lines HONE1, HNE2 and CNE2 that have silenced circ_2157. Compared with the NC group, the proliferation rate of cells in the siRNA group is obvious Slowing down, i.e. silencing circ_2157, suppressed the proliferation of nasopharyngeal carcinoma cells. That is, inhibiting circ_2157 can treat nasopharyngeal carcinoma, which has far-reaching clinical significance and important prospects for promotion and application.

The invention relates to a compound fresh-preserving agent, which is prepared by the following raw materials in proportion by weight: 5-20 nisin from streptococcus lactis, 10-60 sodium dehydroacetate, 5-50 Glucono- beta-lactone, 5-40 Sodium Erythorbate, 5-50 sodium chloride, and 1-50 sodium tripolyphosphate. The compound fresh-preserving agent of the invention uses the synergistic effect of various raw materials to enable the fresh-preserving effect to be obvious and stabilize sense, chemistry and microorganism indexes of meat and meat products, thus prolonging the shelf life of the meat and the meat products; and the invention has the advantages of wide application product range, convenient processing and use, safety and no toxicity.

The invention provides microRNA used for inducing leukemia cell differentiation and application thereof in the induction of erythroid differentiation of tumor cell lines K562. The microRNA not only has the advantages of cheapness, no toxic or side effect, long duration, high transfection efficiency and the like, but also has the characteristics of stronger action and effectiveness of various indexes for tumor cell differentiation compared with a conventional human leukemia cell erythroid differentiation siRNA inducer.

The invention discloses a production method of a high-salt diluted and low-temperature fragrant soy sauce, which comprises the following steps of: (1) curing raw materials including wheat and defatted soybeans, and then uniformly mixing the wheat and the defatted soybeans; (2) inoculating aspergillus oryzae strains to the obtained raw material mixture so as to obtain a yeast; (3) cultivating the yeast for 44-48 hours at a temperature of 25-30 DEG C so as to obtain a mature yeast; (4) adding 2.0-2.5 weight times of saline water into the mature yeast so as to obtain a raw soy sauce mash; (5) moving the raw soy sauce mash to the inside of an airtight fermentation tank for fermenting: firstly fermenting for 23-27 days at a temperature of 13-17 DEG C, then continuing to ferment at a temperature of 28-32 DEG C, and on the 28-32nd days, adding mixed fermentation strains into the fermentation tank and continuing to ferment; in the process of fermentation, stirring by using a compressed air flow; and fermenting for more than six months so as to obtain a mature soy sauce mash; and (6) squeezing and filtering the mature soy sauce mash so as to obtain the low-temperature fragrant soy sauce. According to the invention, the conversion rate of proteins in raw materials is high, and the contents of ammonia nitrogen, total nitrogen, soybean peptides and soybean oligosaccharides in the prepared soy sauce are high.

The invention relates to a method for rapidly culturing a rice suspension cell line, and belongs to the biotechnical field. The method for rapidly culturing the rice suspension cell line includes the following steps: selection of an explant, sterilization of the explant, callus induction, callus subculture, and suspension cell culture. A suspension cell culture medium and a suspension cell subculture treatment technology are improved. During the subculture treatment of suspension cells, the suspension cultured cells are sequentially screened by sterilized screens with the aperture sizes being about 10 meshes, 20 meshes and 40 meshes after the third-time subculture using a liquid medium to filter out large-granule cell clusters and only reserve cell clusters with uniform size, and the good embryogenic suspension cell line is established through the method in 1.5-2 months. The method has the advantages of low cost, simplicity in operation, shortening of the test cycle, and reduction of the suspension cell line contamination risk, is a new efficient and rapid method, and provides a platform for the relevant study of the rice suspension cell line used as an experimental material.

The invention relates to the technical field of toxoplasma gondii vaccines, in particular to a toxoplasma gondii attenuated live vaccine with deletion of AP2IV-1 gene and a construction method thereof. The invention provides a toxoplasma gondii strain with deletion of the AP2IV-1 gene. The amino acid sequence of AP2IV-1 is shown in SEQ ID NO.1.. Compared with a starting insect strain, the AP2IV-1-gene-deleted insect strain is remarkably reduced in toxicity, and the AP2IV-1-gene-deleted insect strain hardly has pathogenicity in a mouse body and has relatively high safety to a host. The AP2IV-1-gene-deleted strain can provide long-term effective immune protection for acute and chronic reinfection of different types of high-dose toxoplasma gondii, and has the potential of becoming an attenuated live vaccine for preventing animal toxoplasmosis.

The invention belongs to the technical field of tumor molecular biology, in particular to an application of circ_ADARB1 in preparing a nasopharyngeal carcinoma therapeutic preparation and a therapeutic preparation. For the first time, we found that circ_ADARB1 may become a potential target for nasopharyngeal carcinoma treatment. As the siRNA has a good silencing effect, the siRNA designed for a splicing site at the head-tail junction of the circular RNA is adopted to interfere with circ_ADARB1, scratch healing experiments and matrix gel invasion experiments are carried out in nasopharyngeal carcinoma cell lines HNE2 and CNE2, compared with a control group, the invasion and migration ability of cells in circ_ADARB1 group knocked down by the siRNA are obviously weakened, namely, the silencing circ_ADARB1 inhibits the invasion and metastasis of nasopharyngeal carcinoma cells. The application shows that inhibition of circ_ADARB1 can treat nasopharyngeal carcinoma and has far-reaching clinical significance and important popularization and application prospects.