A kind of rapid culture method of rice suspension cell line

Abstract

The invention relates to a method for rapidly culturing a rice suspension cell line, and belongs to the biotechnical field. The method for rapidly culturing the rice suspension cell line includes the following steps: selection of an explant, sterilization of the explant, callus induction, callus subculture, and suspension cell culture. A suspension cell culture medium and a suspension cell subculture treatment technology are improved. During the subculture treatment of suspension cells, the suspension cultured cells are sequentially screened by sterilized screens with the aperture sizes being about 10 meshes, 20 meshes and 40 meshes after the third-time subculture using a liquid medium to filter out large-granule cell clusters and only reserve cell clusters with uniform size, and the good embryogenic suspension cell line is established through the method in 1.5-2 months. The method has the advantages of low cost, simplicity in operation, shortening of the test cycle, and reduction of the suspension cell line contamination risk, is a new efficient and rapid method, and provides a platform for the relevant study of the rice suspension cell line used as an experimental material.

Description

technical field

[0001] The invention relates to a method for rapidly cultivating rice suspension cell lines, which belongs to the field of biological technology. Background technique

[0002] Rice is one of the most important food crops in the world, the staple food of more than 50% of the world's population, and also one of the model plants for genetics and genome research. The wild rice verruca is mainly distributed in Yunnan Province and Hainan Province of my country. Because wild rice has been in the wild state for a long time, it has withstood natural selection of various disasters and adverse environments, and has strong stress resistance. It is a natural gene pool and maintains favorable genes that cultivated rice does not have or has disappeared.

[0003] Suspension cell lines have a unified source and a consistent genetic background, and there is no influence of individual differences in plant research; the growth conditions are easy to control, the physiological s...