Antineoplastic medicine prepared through gelsmium elegans medicinal materials

An anti-tumor drug and anti-tumor technology, applied in the field of anti-tumor drugs, can solve the problems of the clinical application constraints of the original medicinal materials and crude extracts of Gelkins

Inactive Publication Date: 2017-03-29
FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide an anti-tumor drug prepared by using the medicinal material of gills, aiming to solve the problem that the clinical application of the raw medicinal material and the crude extract of the gills are greatly restricted

Method used

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  • Antineoplastic medicine prepared through gelsmium elegans medicinal materials
  • Antineoplastic medicine prepared through gelsmium elegans medicinal materials
  • Antineoplastic medicine prepared through gelsmium elegans medicinal materials

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preparation example Construction

[0031] Such as figure 1 As shown, the preparation method of the anti-tumor drug prepared by using the medicinal material of gecko according to the embodiment of the present invention comprises the following steps:

[0032] S101: Take fresh or dried decoction pieces of vine stems, roots and leaves, and add ethanol (50-95% ethanol concentration, preferably 70%) in an amount 6 to 12 times the mass of fresh or dry decoction pieces of vine stems, roots and leaves, or Methanol, acetone, ethyl acetate and other organic solvents, soaked and extracted, the extract was filtered while it was hot, and the organic solvents such as ethanol were recovered under reduced pressure with a vacuum rotary evaporator, extracted 1 to 3 times, the filtrates were combined, concentrated until no alcohol smell, added Dissolve a certain amount of acid solution such as hydrochloric acid or sulfuric acid and adjust the pH to acidity 1-5. Do this for 3-6 times until the alkaloid test of the supernatant is ne...

Embodiment 1

[0040] (1) Take the fresh or dried decoction pieces of the Glycyrrhizae vine, add ethanol (50% to 95% ethanol concentration, preferably 70%) or methanol in an amount 6 to 12 times the mass of the fresh or dry decoction pieces of the Glycyrrhizae vine, soak and extract, and the extract Suction filtration while it is hot, recover ethanol and other organic solvents under reduced pressure with a vacuum rotary evaporator, extract 1 to 3 times, combine the filtrates, concentrate until there is no alcohol smell, add a certain amount of hydrochloric acid to dissolve and adjust the pH to acidity 1 to 5, This operation was performed 3 to 6 times until the alkaloid test of the supernatant was negative or weakly positive, and the supernatant was combined.

[0041] (2) Add sodium hydroxide to adjust the pH to 8-13 alkaline, and extract the alkalized solution with 2-6 times the amount of chloroform for 3-6 times until the liquid alkaloid test after alkalization is negative or weakly positive...

Embodiment 2

[0046] (1) Take fresh or dried fresh or dried decoction pieces of Glycyrrhiza foliage, add ethanol (50% to 95% ethanol concentration, preferably 70%) or acetone in an amount 6 to 12 times the mass of fresh or dry decoction pieces of Glycyrrhiza foliage, soak and extract, The extract is filtered while it is hot, and organic solvents such as ethanol are recovered under reduced pressure with a vacuum rotary evaporator, extracted for 1 to 3 times, the filtrate is combined, concentrated until there is no alcohol smell, dissolved in an acid solution with a certain amount of sulfuric acid, and the pH is adjusted to an acidic value of 1-3. 5. Do this for 3 to 6 times until the alkaloid test of the supernatant is negative or weakly positive, and then combine the supernatant.

[0047] (2) Add potassium hydroxide to adjust the pH to 8-13 alkaline, and use 2-6 times the amount of dichloromethane petroleum ether organic solvent to extract the alkalized solution for 3-6 times until the alkal...

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Abstract

The invention discloses an antineoplastic medicine prepared through gelsmium elegans medicinal materials. The antineoplastic medicine comprises the following steps that fresh gelsmium elegans rattan stems and/or roots and/or leave and/or dry medicinal slices are taken, steeping and extracting are conducted, ethyl alcohol is recycled by reducing pressure, concentration is conducted until the alcohol taste is eliminated, acid is added for dissolving, PH is adjusted, and upper clear liquid is taken; alkali is added, PH is adjusted to be 9-10, solution extraction is conducted, organic solvent is recycled, and gelsmium elegans total alkaloid extractum is obtained; the extractum is dissolved through methyl alcohol and the like and blended with silica gel, drying and crushing are conducted, the upper silica gel is prepared to a column, gradient elute is conducted by organic solvent, and rough-wrought portions of sempervirine are obtained; and high-middle-pressure phase reversal silica gel column preparation is conducted. The gelsmium elegans total alkaloid begins to exert influences on activity of tumour cells after being added for four hours, and the multiplication speed of the tumour cells is lowered a little compared with a blank group; and the sempervirine begins to exert influences on activity of the tumour cells after being added for five hours, the sempervirine with a low dosage can lower the multiplication speed of the tumour cells, and the sempervirine with a high dosage can kill the tumour cells.

Description

technical field [0001] The invention belongs to the technical field of antineoplastic drugs, and in particular relates to an antineoplastic drug prepared by using the medicinal material of gecko. Background technique [0002] Gelkins is a well-known toxic traditional Chinese medicine, which has certain curative effects in analgesic and anti-tumor, but because of its high toxicity, the clinical application of its raw materials and crude extracts is greatly restricted. In recent years, more than 40 monomers such as kinesin, kelesine A, and sargosine A have been extracted, isolated and purified from the medicinal materials of kinesin, such as kelesine, kelesine A, The analgesic and antitumor effects and preparations of chemical components such as 1-methoxygesine have been reported. [0003] Sempervirine (sempervirine) is an indole alkaloid isolated from Gelkins, and the anti-tumor active drug of Gelsynine is a technical blank. Contents of the invention [0004] The purpose ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/475A61K36/56A61P35/00
CPCA61K31/475A61K36/56
Inventor 吴水生
Owner FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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