Stem cell cryopreservation liquid and preparation and use method thereof
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A technology of stem cells and cryopreservation solution, which is applied in the field of cell biology and can solve problems such as unsatisfactory results
Inactive Publication Date: 2020-10-30
海南济民博鳌国际医院有限公司
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Problems solved by technology
The current use of stem cell cryopreservation solutions is not ideal. Therefore, it is very important to develop a stem cell cryopreservation solution that can effectively prevent cell low temperature damage and maintain cell viability.
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Embodiment 1
[0031] A stem cell cryopreservation solution, comprising the following components: by mass percentage,
[0032] DMSO 5%, Glycerin 1%, Trehalose 2%, Ethanol 2%, N-Lauryl Sarcosine 0.05%, Calcium Phosphate 1.0%, Polyporine C 0.03%, Trigonelline 0.03%, 1,8 - 0.04% eucalyptol, and the balance is DMEM medium.
Embodiment 2
[0034] A stem cell cryopreservation solution, comprising the following components: by mass percentage,
[0035] DMSO 5%, Glycerin 1%, Trehalose 2%, Ethanol 2%, N-Lauryl Sarcosine 0.07%, Calcium Phosphate 0.7%, Polyporine C 0.03%, Trigonelline 0.03%, 1,8 - 0.03% eucalyptol, and the balance is DMEM medium.
Embodiment 3
[0037] A stem cell cryopreservation solution, comprising the following components: by mass percentage,
[0038] DMSO 0%, Glycerin 2%, Trehalose 1%, Ethanol 3%, N-Lauryl Sarcosine 0.06%, Calcium Phosphate 0.8%, Polyporine C 0.04%, Trigonelline 0.05%, 1,8 - 0.02% eucalyptol, and the balance is DMEM medium.
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Abstract
The invention discloses a stem cell cryopreservation liquid as well as a preparation method and a use method thereof. The stem cell cryopreservation liquid is prepared from 0 to 20 percent of DMSO, 1to 2 percent of glycerol, 1 to 2 percent of trehalose, 2 to 5 percent of ethanol, 0.05 to 0.07 percent of N-dodecanoyl sarcosine, 0.7 to 1.0 percent of calcium phosphate, 0.03 to 0.06 percent of polyporus acid C, 0.02 to 0.05 percent of trigonelline, 0.01 to 0.04 percent of 1, 8-cineole, and the balance of DMEM culture medium. After the stem cell cryopreservation liquid is used for cryopreservation, the stem cell resuscitation rate reaches 90% or above, and compared with non-cryopreserved cells, the proliferation speed is not obviously reduced and even increased.
Description
technical field [0001] The invention relates to the technical field of cell biology, in particular to a stem cell cryopreservation solution and a method for preparing and using the same. Background technique [0002] Stem cells are a type of cells with high self-renewal proliferation and multi-directional differentiation potential, including neural stem cells, mesenchymal stem cells, etc., which can differentiate into various types of cells under certain conditions. Stem cells have multiple uses and functions, and can be used to study the growth and development of tissues and organs in vitro, to establish disease models, to screen drugs, and to treat diseases. For example, mesenchymal stem cells can differentiate into fat, cartilage, muscle, tendon, ligament, nerve, liver, heart muscle, endothelial and other tissue cells under specific induction conditions in vivo or in vitro, and can be used as ideal seed cells for aging and repair of tissue and organ damage caused by dise...
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