Catalpa tissue culture rapid propagation method

Abstract

The invention provides a catalpa tissue culture rapid propagation method, and relates to the technical field of plant tissue culture. A catalpa tissue culture medium comprises a primary culture medium, a proliferation culture medium and a rooting culture medium. The primary culture medium and the proliferation culture medium are Qj culture mediums: N6+6-BA 1.25 mg/L+IBA 0.07 mg/L+ agar 6.5 g/L+ sucrose 30g/L, and the rooting culture medium selects a Qg culture medium: 1/2MS+6-BA0.01mg/L+IBA 0.5mg/L+ agar 6.5g/L+ sucrose 15g/L. The method catalpa tissue culture rapid propagation method using the catalpa tissue culture medium includes the following steps: 1) obtaining sterile seedlings, to be more specific, selecting new shoots, treating the lengths, cleaning, disinfecting, sterilizing, cutting, inoculating into the Qj mediums, and cultivating to obtain sterile seedlings, 2) performing proliferation culture, to be more specific, treating the lengths of the sterile seedlings, transferringthe sterile seedlings to the Qj mediums, and cultivating to obtain subculture seedlings; 3) performing rooting culture, to be more specific, treating the subculture seedlings, inoculating the subculture seedlings into the Qg culture medium, and cultivating to obtain rooted seedlings; and 4) hardening seedlings and transplanting, to be more specific, transferring the rooted seedlings to a greenhouse for hardening the seedlings, transplanting the seedlings to an aperture disk, and transplanting after cultivation. The catalpa tissue culture rapid propagation method has the beneficial effects that effective multiplication rate of rooting culture is high, seedling rate is high, and factory production can be facilitated.

Description

technical field [0001] The invention provides a method for tissue culture and rapid propagation of golden wire, which relates to the technical field of plant tissue culture. Background technique [0002] Catalpa bungei'jinsiqiu'., a tall deciduous tree belonging to the genus catalpa of the family Bignoniaceae, is an extremely important local species of high-grade wood in catalpa (Catalpa bungei). It is named after the golden thread. Due to its obvious variation characteristics and certain adaptation areas, Zhang Junpei et al. believed that Catalpa is a geographical variety of Catalpa (Zhang Junpei et al., 2008). Not only is the material tough and the pattern is beautiful, but it also has the advantages of straight trunk, strong adaptability, and fast growth. It is the preferred tree species for agricultural and forestry intercropping, afforestation and landscaping. At present, the production of golden catalpa is mainly carried out by means of root cuttings and grafting. Th...

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Problems solved by technology

[0006] The present invention provides a high effective value-added rate and a high rate of seedling growth for the tissue culture medium and rapid propagation method of the golden silk weed, which solves the problem of the small input-output ratio of the golden silk tissue culture in the prior art and the poor rapid propagation effect. ideal technical questions

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