Method for removing endotrophic bacteria in tissue culture system of nandina with red leaves

A technology of tissue culture and endophytic bacteria, which is applied in the field of plant cultivation, can solve the problems not mentioned above, and achieve the effects of no vitrification, normal performance and normal proliferation coefficient

Active Publication Date: 2013-02-20
ZHEJIANG SENHE SEED +1
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Problems solved by technology

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Method used

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Embodiment Construction

[0016] Now, the present invention will be further described in conjunction with the embodiments of the present invention.

[0017] 1) From the tissue culture seedlings of Nantian bamboo in a proliferating state, select the base of the plant in the culture medium where there is cloudy smudged endophyte contamination, the smudged part has not yet expanded to the surface of the medium, and the medium has not changed color significantly tissue culture seedlings;

[0018] 2) On the ultra-clean workbench, use sterilized tweezers to take out the selected tissue culture seedlings from the bottle, place them in a sterile stainless steel plate, cut off the 1cm part of the seedling tip with a sterilized blade, and cut off Do not touch the base of the seedling, place it in another aseptic stainless steel plate and strip the stem tip;

[0019] 3) Cut off the 0.3cm part of the apex of the stem tip, and quickly add 50 mg·L -1 Penicillin G-K or 1.5 g·L -1 50% carbendazim wettable powder i...

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Abstract

The invention provides a method for removing endotrophic bacteria in a tissue culture system of a nandina with red leaves, which belongs to the technical field of plant cultivation. The method is characterized by comprising the following steps of: (1) selecting tissue culture seedlings with endotrophic bacterium infection in seedling bases in a culture medium from the tissue culture seedlings of the nandina with red leaves in a proliferated state; (2) taking out the tissue culture seedlings with endotrophic bacteria on an ultraclean workbench, cutting off apexes and peeling off stem apexes; (3) inoculating the peeled stem apexes into an inducing differentiation culture medium added with bacteriocidal substances; an (4) cutting off adventitious buds differentiated from the stem apexes after the stem apexes are cultured for a section of time, transferring into a proliferation culture medium to be cultured until the tissue culture seedlings grow normally and no endotrophic bacterium pollution is caused. By using the removing method, the removing rate of the endotrophic bacteria reaches 90%, and the obtained nandina with red leaves has excellent quality and broad application prospect.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, in particular to a method for removing endophytes in a tissue culture system of Nandina chinensis. Background technique [0002] Red-leaf Nandina belongs to Berberidaceae Berberidaceae Nandina Nandina A new cultivated variety of "Zhejiang Senhe Seed Industry Co., Ltd." was selected from the Nantian bamboo cultivation group. Red-leaf nandina is an evergreen shrub with a plant height of about 80cm. It grows in clusters and has few branches. The common nandina is 8-10cm in length; the leaves are compound leaves with two rounds and three leaflets, with obtuse apex, cuneate base, entire margin, thin leathery, 3-5cm long, 1.5-2cm wide, and 1.5-3cm petiole. Red-leaved Nandina likes half-shade, and can grow normally under strong light. It likes warm climate, fertile, moist and well-drained soil; the leaves that are exposed to direct sunlight in winter all turn red, and when it encounters cold...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 郑勇平邵春荣范文锋王越杜三峰
Owner ZHEJIANG SENHE SEED
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