A kind of borneol camphor plantlet multiplication and rejuvenation method

A technology of borneol camphor and test-tube seedlings, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of vitrification, low multiplication coefficient, browning, etc., reduce vitrification and increase multiplication coefficient , the effect of alleviating the problem of browning

Inactive Publication Date: 2018-12-07
HUNAN ACAD OF FORESTRY
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The present invention aims at the problems of low multiplication coefficient, vitrification and browning in the long-term in vitro multiplication culture process of borneol camphor, and provides a multiplication method with high multiplication coefficient, effectively reducing vitrification and browning, and improving the quality of test-tube plantlets. Rejuvenation culture method

Method used

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  • A kind of borneol camphor plantlet multiplication and rejuvenation method
  • A kind of borneol camphor plantlet multiplication and rejuvenation method
  • A kind of borneol camphor plantlet multiplication and rejuvenation method

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Experimental program
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Effect test

Embodiment 1

[0027] A, Proliferation culture: Cut out the adventitious buds of borneol camphor camphora camphora with a stem length of 1-2 cm, cut off all the leaves, and inoculate the improved MS (doubling of calcium chloride, nitric acid) at the 1 / 3 of the base of the lateral buds. Ammonium was reduced to 1 / 4 of the original concentration) in the medium (Table 1), and additional mitogen 6-BA 1.0mg / L, auxin NAA 0.05mg / L, gibberellin GA 3 0.3mg / L, anti-browning agent AgNO 3 (See photodecomposition) 1.0mg / L, sucrose 30g / L and carrageenan 6.0g / L, pH=6.0. Culture conditions: After inoculation, culture in a dark environment for 3 days (to avoid the decomposition of silver nitrate), then switch to light culture, light intensity 3000lx, light cycle 12h / d, culture temperature 25±1°C, culture cycle 30 days.

[0028] B. Rejuvenation culture: After three generations of continuous multiplication of tissue culture seedlings, transfer to rejuvenation culture, cut out robust adventitious buds and keep...

Embodiment 2

[0036]Compared with Example 1, the culture period in step A was changed to 25 days, and the culture period in step B was changed to 15 days. At the end of the cultivation period, the average proliferation coefficient of the tissue cultured seedlings reached 9.28, and there was basically no vitrification.

Embodiment 3

[0038] Compared with Example 1, the light intensity in steps A and B is all changed to 2000lx. At the end of the cultivation period, the average proliferation coefficient of the tissue cultured seedlings reached 9.07, and there was basically no vitrification.

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Abstract

The invention relates to a cirmamonun camphora test-tube plantlet propagation and rejuvenation method, which comprises propagation culture and rejuvenation culture of test-tube plantlets. In propagation culture of cirmamonun camphora test-tube plantlets, modified MS is taken as the basic culture medium, mitogen 6-BA (0.8-1.2 mg / L), auxin NAA (0.045-0.055 mg / L), gibberellin GA3 (0.1-0.3 mg / L), and an anti-browning agent AgNO3 (1.0-2.0 mg / L) are added, darkness culture is carried out for 1 to 3 days, then light culture is performed, the culture period is 25 to 35 days; after three continuous generations of propagation culture, rejuvenation culture is carried out, modified 1 / 2 MS is taken as the basic culture medium, auxin NAA (0.045-0.055 mg / L), active carbon (1.0-1.5 g / L), and coconut juice (50-100 mL / L) are added, light culture lasts for 15 to 20 days, and after each three generates of propagation culture, one generation of rejuvenation culture is performed. The provided method can overcome the problems that after long term isolated culture of cirmamonun camphora test-tube plantlets, the propagation coefficient is not high, vitrification and browning happen, and the leaves become yellow and withered. The method has a high propagation coefficient, can reduced vitrification and browning, and is capable of guaranteeing the growth quality of cirmamonun camphora test-tube plantlets.

Description

technical field [0001] The invention belongs to the technical field of tissue culture, and in particular relates to a method for proliferating and rejuvenating borneol camphor test-tube seedlings. Background technique [0002] Borneo camphor is a specific chemical type of camphor tree (Cinnamomum camphora) discovered for the first time in my country - borneol-type camphor. The essential oil of its leaves is rich in d-borneol (natural borneol), which is currently the most suitable plant resource for extracting natural borneol. The discovery of borneol camphor has also filled in the historical blank that my country does not produce natural borneol. In recent years, the propagation of borneol camphor is mainly cutting, and it can also be sown and grafted. However, the traditional propagation method has a low multiplication coefficient, long cycle, and large area, which is far from meeting the needs of large-scale and standardized production of commercial seedlings, and is the m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 何洪城李刘泽木杨夕宽何可丹
Owner HUNAN ACAD OF FORESTRY
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