A kind of borneol camphor plantlet multiplication and rejuvenation method
A technology of borneol camphor and test-tube seedlings, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of vitrification, low multiplication coefficient, browning, etc., reduce vitrification and increase multiplication coefficient , the effect of alleviating the problem of browning
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Embodiment 1
[0027] A, Proliferation culture: Cut out the adventitious buds of borneol camphor camphora camphora with a stem length of 1-2 cm, cut off all the leaves, and inoculate the improved MS (doubling of calcium chloride, nitric acid) at the 1 / 3 of the base of the lateral buds. Ammonium was reduced to 1 / 4 of the original concentration) in the medium (Table 1), and additional mitogen 6-BA 1.0mg / L, auxin NAA 0.05mg / L, gibberellin GA 3 0.3mg / L, anti-browning agent AgNO 3 (See photodecomposition) 1.0mg / L, sucrose 30g / L and carrageenan 6.0g / L, pH=6.0. Culture conditions: After inoculation, culture in a dark environment for 3 days (to avoid the decomposition of silver nitrate), then switch to light culture, light intensity 3000lx, light cycle 12h / d, culture temperature 25±1°C, culture cycle 30 days.
[0028] B. Rejuvenation culture: After three generations of continuous multiplication of tissue culture seedlings, transfer to rejuvenation culture, cut out robust adventitious buds and keep...
Embodiment 2
[0036]Compared with Example 1, the culture period in step A was changed to 25 days, and the culture period in step B was changed to 15 days. At the end of the cultivation period, the average proliferation coefficient of the tissue cultured seedlings reached 9.28, and there was basically no vitrification.
Embodiment 3
[0038] Compared with Example 1, the light intensity in steps A and B is all changed to 2000lx. At the end of the cultivation period, the average proliferation coefficient of the tissue cultured seedlings reached 9.07, and there was basically no vitrification.
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