High-density continuous culture method and device for microalgae

Abstract

The invention belongs to the technical field of microalgae and in particular relates to a high-density continuous culture method and device for the microalgae. The device comprises a photobioreactor circulating unit, a nutrient solution replenishing unit, a ventilating carbon-replenishing unit and a microalgae collecting unit. The method using the device comprises the following steps: (1) inoculating the microalgae; (2) multiplying the microalgae; (3) collecting the microalgae; (4) replenishing a culture medium; (5) repeating the steps (2) to (4). According to the method, the multiplication rate of microalgae cells is adjusted and controlled through timely collecting and replenishing processes, so that the logarithm growth period of the microalgae cells in the photobioreactor circulating unit is longer, the high-density continuous culture of the microalgae is achieved, a microalgae product with higher yield can be obtained, and the yield can be increased by more than 30% in comparison with a conventional batch culture method in the microalgae industry. According to the device, a control valve can adopt a solenoid valve and large-scale centralized control can be achieved.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and in particular relates to a method and device for high-density continuous cultivation of microalgae. Background technique [0002] Microalgae are not only rich in protein, fat and carbohydrates, which are three essential substances for human beings, but also contain various amino acids, vitamins, antibiotics, high unsaturated fatty acids and other biologically active substances. , biochemical reagents, fine chemical products, fuels and other materials are an important way. With the increasing pressure of global resource shortage, the development and utilization of microalgae will be one of the important ways to solve human food resources and energy. [0003] At present, the cultivation of microalgae mainly adopts an open method, which is simple in construction, low in cost and easy to operate. However, there are still many deficiencies in the open photobioreactor: (1) easily affected ...

AI Technical Summary

Problems solved by technology

However, there are still many deficiencies in the open photobioreactor: (1) easily affected by the external environment, it is difficult to maintain a suitable temperature and light; (2) it will be polluted by dust, insects and bacteria, and it is difficult to maintain high-quality single Algae cultivation; (3) light energy and CO 2 The utilization rate is not high, and it is impossible to achieve high-density single algae culture; these factors will lead to low cell culture density, resulting in higher harvesting costs, and the microalgae that can adapt to large pond culture must be able to grow rapidly in extreme environments species of algae such as spirulina, chlorella and salina

However, closed culture is difficult to achieve high-density large-scale continuous production of microalgae

[0004] To sum up, in the prior art, the large-scale continuous culture of microalgae has the problems of large production system, easy to be affected by weather, low yield and high harvesting cost.

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