Method of tissue culture of iris ensata

A technique of tissue culture and Jade cicada, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems affecting the application and promotion of Jade cicada gardens, intensified contradictions between supply and demand of seedlings, weak growth of ramets, etc. , to achieve the effect of shortening the breeding time, increasing the survival rate and vigorous growth

Inactive Publication Date: 2012-07-04
广州市卉通农业科技有限公司
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Problems solved by technology

[0004] Same as other rhizome irises, Japonica has both sexual seeds and asexual ramifications. However, the use of seeds for propagation is slow, the reproduction efficiency is low, and the rate of variation is high, while the use of ramets will cause As a result, the growth potential of ramet seedlings is getting weaker and weaker. The technology of using tissue culture technology to breed Yu Chanhua has not yet been studied, and the popularization of fine varieties has not been completed.

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Embodiment Construction

[0019] The present invention will be further described below in conjunction with the examples. The following examples are only used to illustrate the present invention, but not to limit the present invention.

[0020] The tissue culture method of Yu Chan flower is characterized in that the method comprises the following steps:

[0021] 1. Selection of explants: take the stem segment and sub-nodes of the scapes as explants before the flowering of Yuchania japonica;

[0022] ②Material treatment method: first use a soft brush to gently brush off the dust on the surface of the explant, rinse it with running water for 1 hour, and then irradiate it with an ultraviolet lamp for 20 minutes. Soak in 70-75% ethanol for 10 seconds, then rinse with sterile water, then soak in 0.1% mercuric chloride solution with 2 to 3 drops of soil temperature 80 for 7 minutes, rinse with sterile water for 4 times, and finally Divide the material under sterile conditions, and cut the stem into 0.5-0.6 c...

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Abstract

The invention discloses a method of a tissue culture of iris ensata. The good effects of quick multiplication rate, stable hereditary feature, high reproduction coefficient and exuberant test-tube plantlet growth are reached mainly through the steps of primary culture, secondary culture, multiplication culture, rooting culture and hardening-plantlet transplantation during a tissue culture process, a germinating rate of a callus of the iris ensata is up to 96%, the multiplication multiple is up to 4.35 times, the rooting rate is up to 100%, and the transplanting survival rate is up to 98%.

Description

technical field [0001] The present invention relates to a method for tissue culture of Japonica japonica. Background technique [0002] The genus Iris is a perennial herbaceous plant belonging to the family Iridaceae, widely distributed in the northern temperate zone, with differentiation centers from China to the Middle East (Paleo-Mediterranean). China is very rich in resources of Iris, especially Iris bearded mainly distributed in the southwest, northwest and northeast regions. Iris has wide ecological adaptability, strong resistance, is suitable for cultivation in different habitats, is resistant to extensive management, and has a high reproduction coefficient. After frost in early spring and autumn, it can also be used as feed for cattle and sheep, with extremely wide application prospects. Domestic research on Iris started relatively late, and mainly focused on taxonomic research, and most species are still in a wild state that has not yet been exploited. [0003] J...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李玉弟
Owner 广州市卉通农业科技有限公司
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