Tissue culture propagation method for clematis lanuginosa

Abstract

The invention discloses a tissue culture propagation method for clematis lanuginosa. The excellent effects of high multiplication rate, stable hereditary feature, high reproduction coefficient and vigorous test-tube plantlet growth are achieved through the steps of primary culture, multiplication culture, rooting culture, seedling hardening and transplanting mainly, the germinating rate of the callus of the clematis lanuginosa is up to 98%, the multiplication multiple is up to 5.7 times, the rooting rate is up to 100%, and the final survival rate is up to 96%.

Description

technical field [0001] The invention relates to a tissue culture propagation method suitable for clematis tomentosa. Background technique [0002] Clematis trichomes is a climbing vine, often with opposite single leaves, very rare with three compound leaves; single flowers terminal; apex acute, base tapering, inner surface glabrous, base with three straight midribs and lateral network veins It can be seen that the outside forms a lanceolate band along the three straight midribs, covered with yellow curly hairs, the outside is covered with light downy hairs, and the edges are nearly glabrous; the flowering period is June, and the fruiting period is July-August. Produced in northeast Zhejiang. It often grows in valleys and streamside shrubs at an altitude of 100-400 meters. [0003] At present, no one has studied the technology of using tissue culture to breed clematis, the tissue culture system has not been established, the popularization of fine varieties has not been comp...

AI Technical Summary

Problems solved by technology

[0003] At present, no one has studied the technology of using tissue culture to breed clematis, the tissue culture system has not been established, the popularization of fine varieties has not b...