Tissue culture propagation method for clematis lanuginosa

Abstract

The invention discloses a tissue culture propagation method for clematis lanuginosa. The excellent effects of high multiplication rate, stable hereditary feature, high reproduction coefficient and vigorous test-tube plantlet growth are achieved through the steps of primary culture, multiplication culture, rooting culture, seedling hardening and transplanting mainly, the germinating rate of the callus of the clematis lanuginosa is up to 98%, the multiplication multiple is up to 5.7 times, the rooting rate is up to 100%, and the final survival rate is up to 96%.

Description

technical field

[0001] The invention relates to a tissue culture propagation method suitable for clematis tomentosa. Background technique

[0002] Clematis trichomes is a climbing vine, often with opposite single leaves, very rare with three compound leaves; single flowers terminal; apex acute, base tapering, inner surface glabrous, base with three straight midribs and lateral network veins It can be seen that the outside forms a lanceolate band along the three straight midribs, covered with yellow curly hairs, the outside is covered with light downy hairs, and the edges are nearly glabrous; the flowering period is June, and the fruiting period is July-August. Produced in northeast Zhejiang. It often grows in valleys and streamside shrubs at an altitude of 100-400 meters.

[0003] At present, no one has studied the technology of using tissue culture to breed clematis, the tissue culture system has not been established, the popularization of fine varieties has not been comp...

Examples

Embodiment Construction

[0014] The present invention will be further described below in conjunction with the examples, and the following examples are only used to illustrate the present invention, and do not limit the present invention.

[0015] The tissue culture propagation method of clematis pilosa, the method comprises the following steps:

[0016] (1) Selection of explants: From April to July, the young and tender stems of Clematis tomentosa which grow vigorously are taken as explants;

[0017] (2) Material processing method: first use a soft brush to gently brush off the dust on the surface of Clematis clematis, rinse with running water for 10 minutes, then irradiate with ultraviolet light for 10 minutes, transfer to the ultra-clean workbench, and place the dust on the ultra-clean workbench Soak in 70% ethanol for 10 seconds, then soak in 2-3 drops of 0.1% mercuric chloride solution with a soil temperature of 80°C for 2-3 minutes, then rinse with sterile water for 3-4 times, and finally Divide...

technical field

[0001] The invention relates to a tissue culture propagation method suitable for clematis tomentosa. Background technique

[0002] Clematis trichomes is a climbing vine, often with opposite single leaves, very rare with three compound leaves; single flowers terminal; apex acute, base tapering, inner surface glabrous, base with three straight midribs and lateral network veins It can be seen that the outside forms a lanceolate band along the three straight midribs, covered with yellow curly hairs, the outside is covered with light downy hairs, and the edges are nearly glabrous; the flowering period is June, and the fruiting period is July-August. Produced in northeast Zhejiang. It often grows in valleys and streamside shrubs at an altitude of 100-400 meters.

[0003] At present, no one has studied the technology of using tissue culture to breed clematis, the tissue culture system has not been established, the popularization of fine varieties has not been comp...

Examples

Embodiment Construction

[0014] The present invention will be further described below in conjunction with the examples, and the following examples are only used to illustrate the present invention, and do not limit the present invention.

[0015] The tissue culture propagation method of clematis pilosa, the method comprises the following steps:

[0016] (1) Selection of explants: From April to July, the young and tender stems of Clematis tomentosa which grow vigorously are taken as explants;

[0017] (2) Material processing method: first use a soft brush to gently brush off the dust on the surface of Clematis clematis, rinse with running water for 10 minutes, then irradiate with ultraviolet light for 10 minutes, transfer to the ultra-clean workbench, and place the dust on the ultra-clean workbench Soak in 70% ethanol for 10 seconds, then soak in 2-3 drops of 0.1% mercuric chloride solution with a soil temperature of 80°C for 2-3 minutes, then rinse with sterile water for 3-4 times, and finally Divide...