Tissue culture propagation method of anemone dichotoma

Abstract

The invention discloses a tissue culture propagation method of anemone dichotoma. The method comprises the steps of primary culture, enrichment culture, rooting culture, acclimatization and transplant, achieves the good effects of high multiplication rate, stable inheritable characters, high propagation coefficient and vigorous growth of test-tube plantlets, and ensures that the germinating rate of callus tissues of the anemone dichotoma reaches 99 percent, the proliferation times reaches 8.7, the rooting percentage reaches 100 percent, and the final survival rate reaches 97 percent.

Description

technical field [0001] The invention relates to a tissue culture and propagation method suitable for bifid anemone. Background technique [0002] Anemone dichotoma (scientific name: Anemone dichotoma L.) is a herbaceous plant with a plant height of 35-60 cm. Basal leaves 1, involucre bracts 2, fan-shaped, three-parted near base. Flowers solitary on inflorescence branches; sepals 5, white or pinkish, obovate or elliptic, carpels about 30, ovary oblong, with short styles curving outwards. Achenes flat, ovate or elliptic, with margins and slightly curved persistent styles. Blooms in June. On hills or slopes wet grasslands or forests. Distributed in northern Asia and Europe, Jilin and Heilongjiang, China. [0003] At present, no one has studied the technology of using tissue culture to breed Anemone difidis, the tissue culture system has not been established, the promotion and popularization of fine varieties has not been completed, and the contradiction between supply and ...

AI Technical Summary

Problems solved by technology

[0003] At present, no one has studied the technology of using tissue culture to breed Anemone difidis, the tissue culture system has not yet been established, the promotion and popularization of fine varieties has not been completed, and the contradiction between supply and demand of seedlings has become increasingly serious, which has seriously affected the garden application and promotion of Anemone difidis.