Tissue culture propagation method of anemone dichotoma

A technique of tissue culture of Anemone dichasii, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the aggravated contradiction between supply and demand of seedlings, affect the application and promotion of Anemone dichasium gardens, and the popularization of fine varieties has not yet been completed and other issues to achieve the effect of stable genetic traits

CN104396748AInactive Publication Date: 2015-03-11罗翼
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2015-03-11
Estimated Expiration
Not applicable · inactive patent
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Abstract

The invention discloses a tissue culture propagation method of anemone dichotoma. The method comprises the steps of primary culture, enrichment culture, rooting culture, acclimatization and transplant, achieves the good effects of high multiplication rate, stable inheritable characters, high propagation coefficient and vigorous growth of test-tube plantlets, and ensures that the germinating rate of callus tissues of the anemone dichotoma reaches 99 percent, the proliferation times reaches 8.7, the rooting percentage reaches 100 percent, and the final survival rate reaches 97 percent.
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Description

technical field

[0001] The invention relates to a tissue culture and propagation method suitable for bifid anemone. Background technique

[0002] Anemone dichotoma (scientific name: Anemone dichotoma L.) is a herbaceous plant with a plant height of 35-60 cm. Basal leaves 1, involucre bracts 2, fan-shaped, three-parted near base. Flowers solitary on inflorescence branches; sepals 5, white or pinkish, obovate or elliptic, carpels about 30, ovary oblong, with short styles curving outwards. Achenes flat, ovate or elliptic, with margins and slightly curved persistent styles. Blooms in June. On hills or slopes wet grasslands or forests. Distributed in northern Asia and Europe, Jilin and Heilongjiang, China.

[0003] At present, no one has studied the technology of using tissue culture to breed Anemone difidis, the tissue culture system has not been established, the promotion and popularization of fine varieties has not been completed, and the contradiction between supply and ...

Examples

Embodiment Construction

[0014] The present invention will be further described below in conjunction with the examples, and the following examples are only used to illustrate the present invention, and do not limit the present invention.

[0015] The tissue culture propagation method of Anemone bifidum, the method comprises the following steps:

[0016] (1) Selection of explants: From April to July, the young and tender stems of Anemone bifidum, which grow robustly, are used as explants;

[0017] (2) Material processing method: first use a soft brush to gently brush off the dust on the surface of the bifid anemone, rinse with running water for 3 minutes, then irradiate with ultraviolet light for 20 minutes, transfer to the ultra-clean workbench, and put it on the ultra-clean workbench First soak in 70% ethanol for 10 seconds, then transfer to 0.1% mercuric chloride solution with 2 drops of soil temperature 80 and soak for 2 minutes, then rinse with sterile water for 2 times, and finally divide the mat...