Grafting method for model arabidopsis thaliana

Abstract

The invention discloses a grafting method for model arabidopsis thaliana.The method comprises the following steps that an arabidopsis thaliana plant with the seedling age of 6-8 days, thick and strong hypocotyl explant, consistent growth vigour and a main leaf emerging newly is used for grafting; stock and scion materials used for grafting are placed in the middle of 1.2% sepharose gel, the hypocotyl explant is transversely cut, and it is ensured that the hypocotyl explant part of the scion material is short and the hypocotyl explant part of the stock material is long; material notches are in tight contact on the sepharose gel, a 1 / 2MS+3% saccharose solid culture medium with the area larger than the stock root area is cut to cover the stock root, and a grafting body is formed; the grafting body is placed in the environment under the temperature of 21 DEG C to be vertically cultured for 7 days; after grafting notches are healed, the grafting body is transferred into a water culture device with 1 / 4MS as a liquid culture medium to be cultured.The problems that operation is difficult, notches are not round or normal, scion side roots are frequently produced, and the grafting survival rate is low due to the fact that arabidopsis thaliana plants are thin and weak are solved.

Description

technical field [0001] The invention relates to the field of plant grafting, in particular to a grafting method of the model plant Arabidopsis thaliana. Background technique [0002] Research on the interaction mechanism between rootstock and scion of horticultural crops is of great significance for the scientific selection of rootstock and scion combinations. However, due to the lack of understanding of the genetic background of horticultural crops, further elucidation of the molecular mechanism of rootstock-ear interaction is limited. The model plant Arabidopsis thaliana is propagated by seeds, with neat emergence, short growth cycle and clear genetic background. It is a very suitable material for molecular mechanism research. There are few reports on Arabidopsis grafting technology, and due to the small size of Arabidopsis plants, thin hypocotyls, and soft materials, the following problems are likely to exist during the grafting process, which ultimately reduces the surv...

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Problems solved by technology

There are few reports on Arabidopsis grafting technology, and due to the small size of Arabidopsis plants, thin hypocotyls, and soft materials, the following problems are likely to exist during the grafting process, which ultimately reduces the survival rate of grafting:

[0003] The Arabidopsis plants are small and the hypocotyls are thin and weak, so it is difficult to perform grafting operations. How to cultivate and obtain Arabidopsis plants with strong hypocotyls suitable for grafting operations is an urgent problem to be solved

When cutting the hypocotyl of Arabidopsis grafting, the incision is easy to be flattened by the blade, it is difficult to keep the incision round, and the grafting surface becomes smaller, which affects the healing of the grafting interface

The material of Arabidopsis thaliana is soft, and it cannot be cultivated upright like woody plants after grafting. Horizontal cultivation is the most used cultivation method, but when cultivated horizontally, because the leaves of Arabidopsis are opposite, the growth of scion leaves is very easy to cause The dislocation of the grafting interface reduces the survival rate of grafting

After grafting, the growth of rootstock roots will inevitably have a relatively low period, and at this time, the scion part is very easy to grow lateral roots from the hypocotyl, thereby ensuring the growth of its own plants, and the germination of lateral roots will affect the grafting interface. The healing of rootstock and the growth of main root will reduce the survival rate of grafting

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