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Fast lavandulol regeneration

A lavender, fast technology, applied in the field of bioengineering, can solve the problems of easy variation in morphological and chemical characteristics, high cost of planting lavender, slow reproduction speed, etc., and achieve the effects of shortening production cycle, strong practical value, and fast growth rate.

Inactive Publication Date: 2006-08-16
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows people who want to grow more plants quickly without having to wait longer periods than usual or requiring much manpower. It uses specific techniques that induce stem cells from plant roots into tiny pieces called cotyledons (the main part) which are then used during various stages of growing new crops such as vegetables or fruits. These small parts help speed up the process while still producing good quality food products at lower cost compared to traditional ways of breeding.

Problems solved by technology

The technical problem addressed in this patented text relates to developing improved methods for growing lavenders without relying heavily upon naturally occurring sources like seeds from grasses during cultivating processes. Existing techniques involve culturing suspended cells called xianthofervescens lacteus under controlled conditions while controllably generating different types of vegetative fluids including alcoholic glycosides. These techniques require expensive equipment and produce poor yields due to variations between individuals.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0028] Obtaining Lavender Hypocotyl Explants

[0029] a. Pick plump lavender seeds, soak them in warm water at 40°C, continue to soak for 24 hours after the water cools, rinse the soaked lavender seeds with running water for 30 minutes, rinse them with 75% ethanol for 25 seconds, rinse them once with sterile water, and rinse them with 0.2% ethanol Soak in sodium dichloroisocyanurate (UCl) solution for 20 minutes, then rinse with sterile water 4-5 times, inoculate the seeds in 0.4% agar-solidified MS solid medium, place at 25±1°C, light for 8 hours, The culture was carried out under dark culture conditions for 16 hours, and the light intensity was 1500 lx. After 15-20 days, the seeds began to germinate, and the germination rate was 53%.

[0030] Direct Differentiation of Lavender Hypocotyl Explants to Adventitious Buds

[0031] b. Take the hypocotyls of the sterile seedlings that have undergone seed germination and cut them into small sections of ab...

Embodiment 2

[0036] Obtaining Lavender Hypocotyl Explants

[0037] a. Pick plump lavender seeds, soak them in warm water at 40°C, continue to soak for 24 hours after the water cools, rinse the soaked lavender seeds with running water for 30 minutes, rinse them with 75% ethanol for 25 seconds, rinse them once with sterile water, and rinse them with 0.2% ethanol Soak in sodium dichloroisocyanurate (UCl) solution for 20 minutes, then rinse with sterile water 4-5 times, inoculate the seeds in 0.4% agar-solidified MS solid medium, place at 25±1°C, light for 8 hours, The culture was carried out under dark culture conditions for 16 hours, and the light intensity was 1500 lx. After 15-20 days, the seeds began to germinate, and the germination rate was 53%.

[0038] Direct Differentiation of Lavender Hypocotyl Explants to Adventitious Buds

[0039] b. Take the hypocotyls of the sterile seedlings that have undergone seed germination and cut them into small sections of abo...

Embodiment 3

[0044] Obtaining Lavender Hypocotyl Explants

[0045] a. Pick plump lavender seeds, soak them in warm water at 40°C, and continue soaking for 24 hours after the water cools down. Rinse the soaked lavender seeds with running water for 30min, rinse with 75% ethanol for 25s, rinse once with sterile water, soak for 20min with 0.2% sodium dichloroisocyanurate (euchlorozine) solution, and then rinse with sterile water 4-5 times, the seeds were inoculated in 0.4% agar-solidified MS solid medium, placed at 25±1°C, 8h light, 16h dark culture conditions, and the light intensity was 1500lx. After 15-20 days, the seeds began to germinate. The germination rate was 53%.

[0046] Direct Differentiation of Lavender Hypocotyl Explants to Adventitious Buds

[0047] b. Take the hypocotyls of the sterile seedlings that have undergone seed germination and cut them into small sections of about 5mm, place them horizontally, and insert 0.5% agar to solidify MS+IAA 0.1mg·L ...

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Abstract

A method for fast regeneration of lavender includes such steps as taking the hypocotyl from the germinated seed of lavender, direct differentiating in the regenerating solid culture medium to generate adventitious bud, and rooting in the rooting solid culture medium to obtain complete plant. Its advantages are short period (60 days), and high germination percentage (46.7%) and rooting rate (95%).

Description

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Claims

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Application Information

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Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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