Method for producing polygalacic acid with milkwort feather shaped root system culture

Abstract

The invention relates to a method for cultivating and producing polygalacic acid with polygala root hair-shaped roots, which uses polygala root hypocotyl and blades as an explantation and converts by activating hair-shaped root agrobacterium, and leads T-DNA of hair-shaped root agrobacterium Ri plasmid to be integrated to polygala root nucleus DNA for expressing and forming hair-shaped roots, and polygala root hair-shaped roots are gained through plural times of subinoculation. The polygala root hair-shaped roots which are gained contain the same components of polygalacic acid (namely, senega-saponin) as that of traditional Chinese medicine polygala root, and the content is not lower than the wild traditional Chinese medicine polygala root. The invention can be used as new medicine resources to provide polygala root products, which solves the practical problems that the demand amount of polygala root is increased year by year, and wild resources are lacked and the quality of cultivated breeds is reduced, which brings practical problems to clinic, Chinese patent drug processes and export.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, in particular to a method for efficiently inducing and cultivating polygala hairy root system to produce polygalic acid. Background technique [0002] Polygala was first recorded in "Shen Nong's Materia Medica", which said: "The whole plant can improve the wisdom and strengthen the will, so it is called Polygala. It is mainly used for coughing and injuring, replenishing deficiencies, eliminating evil spirits, benefiting nine orifices, benefiting wisdom, being smart about eyes and ears, not forgetting, Strong ambition and double strength". Li Shizhen recorded in "Compendium of Materia Medica": "Polygalae has two types of large leaves and small leaves, which enter the kidney meridian of foot Shaoyin, not the medicine of the heart meridian. Its function is dedicated to strengthening the will and improving essence, and curing forgetfulness." "Pharmacopoeia of the People's Republic of China" (2005 ...

AI Technical Summary

Problems solved by technology

According to the survey of the production area, the main reasons are the reduction of wild resources, the destruction of resources, the shrinkage of the production area, poor regeneration (polygala seeds are small, spread by insects such as ants, and natural regeneration is not easy) and home planting difficulties (polygala likes to be born in sufficient sunlight, In places with deep, fertile, neutral or slightly alkaline soil, it takes one year to germinate and grow, and the growth period takes four years. A few places have successfully studied artificial cultivation, but there are still problems in large-scale promotion) and other reasons

In view of the huge demand and high value of Polygala in the market, the limited wild resources and serious damage, and the artificial planting of Polygala with long time, small scale and low yield, the theory and means of modern biotechnology are applied to solve the problem that medicinal plant resources cannot meet market demand. question