Anti-aging composition containing roxburgh rose peptide as well as preparation method and application of anti-aging composition

Abstract

The invention discloses an anti-aging composition and a preparation method thereof, and belongs to the technical field of anti-aging of cosmetics. The anti-aging composition is prepared from the following components in percentage by mass: 0.2 to 0.6 percent of roxburgh rose peptide, 1.1 to 1.3 percent of hydrolyzed abelmoschus esculentus extract, 0.7 to 0.9 percent of ligusticum wallichii root extract, 1.5 to 1.7 percent of silybum marianum seed oil and the balance of auxiliary materials, the roxburgh rose peptide is prepared by a two-step enzymolysis method, and the hydrolyzed abelmoschus esculentus extract is prepared by a two-step enzymolysis method. The ligusticum wallichii root extract is obtained through supercritical CO2 extraction. Performance tests show that the composition can significantly inhibit the activity of elastase, the inhibition rate reaches 70% or above, the composition has excellent DPPH free radical scavenging capacity, the scavenging rate reaches 80% or above, all the components are synergistic, skin aging can be effectively delayed, and the composition has good application prospects.

Description

Technical Field

[0001] The present invention belongs to the technical field of daily cosmetics, and in particular relates to an anti-aging composition containing roxburghii peptide, and a preparation method and application thereof. Background Art

[0002] Skin aging results from the combined effects of endogenous factors (such as aging and genetic regulation) and exogenous factors (such as ultraviolet radiation, environmental pollution, and oxidative stress). It manifests itself as decreased skin elasticity, increased wrinkles, and sagging. Its core mechanisms involve two key pathways: first, overactivation of elastase leads to the degradation of skin elastic fibers, destroying the skin's supporting structure and causing a loss of elasticity; second, oxidative stress triggers the accumulation of free radicals, leading to oxidative damage to lipids and proteins and accelerating the aging process.

[0003] Currently, most anti-aging products on the market rely on a single active ingredient (such as vitamins and plant extracts), resulting in limited efficacy and mechanism of action. While some products attempt to compound ingredients, they struggle to achieve the desired anti-aging effect due to poor synergy between ingredients and low retention of active ingredients. Furthermore, traditional extraction or preparation methods (such as organic solvent extraction and high-temperature extraction) can easily destroy the structure of the active ingredient, further impacting product efficacy.

[0004] Therefore, it is of great significance to develop an anti-aging composition that has multi-component synergistic effects, retains complete active ingredients, and has both elastase inhibition and antioxidant effects. Summary of the Invention

[0005] In response to the problems existing in the prior art of anti-aging products, such as single efficacy, poor ingredient synergy, and low active ingredient retention rate, the present invention discloses the following technical solutions:

[0006] In a first aspect, the present invention provides an anti-aging composition, comprising roxburghii peptide, hydrolyzed amla extract, ligusticum chuanxiong root extract, and silybum marianum seed oil, as well as cosmetically acceptable excipients and solvents.

[0007] Preferably, the composition contains the following components by mass percentage:

[0008]

[0009]

[0010] Further preferably, the preparation method of the roxburghii peptide comprises the following steps:

[0011] Step 1.1. Crush the roxburghii fruit into a homogenate, add deionized water at a solid-liquid ratio of 1:(5-10) g / mL, adjust the pH to 4.5-5.0, stir in a 45-55°C water bath for 25-35 minutes, then centrifuge to collect the precipitate, and wash 2-3 times with deionized water to obtain a crude roxburghii protein extract;

[0012] Step 1.2. The crude protein extract of the roxburghii was used as a substrate for a two-step enzymatic hydrolysis method. First, 3-5% of a composite enzyme 1 consisting of a neutral protease, kiwi protease, and papain was added to the substrate by weight. The enzyme was hydrolyzed at 45-55°C and pH 6.5-7.0 for 2-4 hours. After the hydrolysis was completed, the enzyme was inactivated in a water bath at 80-90°C for 10-15 minutes. The inactivated enzyme mixture was used as a substrate. 3-5% of a composite enzyme 2 consisting of an aminopeptidase and bromelain was added by weight of the substrate. The enzyme was hydrolyzed at 45-55°C and pH 6.5-7.0 for 2-4 hours. After the hydrolysis was completed, the enzyme was inactivated in a water bath at 80-90°C for 10-15 minutes. The supernatant was collected by centrifugation.

[0013] Step 1.3. The supernatant was ultrafiltered through an ultrafiltration membrane with a molecular weight cutoff of 3000Da, and the permeate was further ultrafiltered through a 500Da molecular weight cutoff ultrafiltration membrane to obtain a retentate. 1%-2% activated carbon was added to the retentate, and the mixture was stirred at 45-50°C for 20-30 minutes. The activated carbon was removed by filtration and then concentrated under reduced pressure and freeze-dried to obtain the roxburghii peptide.

[0014] More preferably, the neutral protease activity is 5×10 4 U / g, kiwifruit protease activity 5×10 3 U / g, papain activity 2×10 4 U / g, complex enzyme 1 composed of neutral protease, kiwi protease and papain in a mass ratio of 1:1:1.

[0015] More preferably, the aminopeptidase activity is 5×10 3 U / g, bromelain enzyme activity 1×10 5 U / g, complex enzyme 2 composed of aminopeptidase and bromelain in a mass ratio of 1:1.

[0016] Further preferably, the preparation method of the Chuanxiong root extract comprises the following steps:

[0017] Step 2.1. Grind the dried Chuanxiong root through a 60-mesh sieve to obtain Chuanxiong root powder.

[0018] Step 2.2. Load the Chuanxiong root powder into a supercritical CO2 extraction vessel for extraction and separation to obtain an extract;

[0019] Step 2.3. Filter the extract through a 0.22 μm microfiltration membrane to obtain the Chuanxiong root extract.

[0020] Further preferably, the extraction parameters in step 2.2 are: extraction kettle pressure 18-22 MPa, extraction temperature 30-40°C, CO2 flow rate 15-18 L / h, extraction time 1-1.5 h; the separation parameters are: pressure 6-10 MPa, temperature 45-55°C, separation time 2 h, to obtain an extract.

[0021] In a second aspect, the present invention provides use of the anti-aging composition described in the first aspect in the preparation of a skin care product having antioxidant, anti-aging, whitening, firming and / or anti-wrinkle effects.

[0022] Preferably, the anti-aging composition is added to the skin care product in an amount of 5-10 wt %.

[0023] In the present invention:

[0024] Through a two-step enzymatic hydrolysis method (combined hydrolysis with neutral protease, kiwi protease, and papain, followed by combined hydrolysis with aminopeptidase and bromelain), small-molecule roxburghii peptides with molecular weights ranging from 500 to 3000 Da are obtained. These peptides are rich in antioxidant amino acid residues that can directly scavenge free radicals while specifically inhibiting elastase activity, reducing elastic fiber degradation and exerting a dual anti-aging effect.

[0025] Hydrolyzed coffee sunflower extract is rich in active ingredients such as vitamin C, soluble fiber, polyphenols, unsaturated fatty acids, etc. It has strong antioxidant ability, can effectively scavenge free radicals, reduce oxidative stress, and delay skin aging. At the same time, unsaturated fatty acids can effectively enhance the skin's moisturizing ability, help the skin absorb and lock in moisture, and keep the skin moisturized for a long time.

[0026] The root extract of Chuanxiong is obtained by supercritical CO2 extraction. It is rich in active ingredients such as volatile oils (such as ligustrazine) and phenolic acids. The volatile oil components can improve skin microcirculation and promote the absorption of active ingredients. Phenolic acids have dual anti-inflammatory and antioxidant effects, which can reduce skin inflammatory reactions caused by oxidative stress and delay the aging process.

[0027] Milk thistle seed oil is rich in flavonolignans, unsaturated fatty acids (such as oleic acid and linoleic acid), and fat-soluble vitamins (vitamin E and vitamin A). Unsaturated fatty acids moisturize the skin, repair the skin barrier, and reduce water loss. Flavonolignans and fat-soluble vitamins act as natural antioxidants, quenching free radicals and synergizing with other water-soluble antioxidants to enhance overall antioxidant capacity.

[0028] Beneficial effects of the present invention:

[0029] 1. The present invention achieves a multi-pathway anti-aging effect of "inhibiting elastase + scavenging free radicals + repairing the barrier + improving circulation" through the scientific combination of roxburghii peptide, hydrolyzed sunflower extract, ligusticum root extract, and milk thistle seed oil. Performance tests show that the elastase inhibition rate of the combination is over 70%, and the DPPH free radical scavenging rate is over 80%, which are significantly better than the single-ingredient control group, and some indicators are better than the positive control;

[0030] 2. The Rosa roxburghii peptide is hydrolyzed using a two-step enzymatic method to avoid high temperature damage to its activity. The Chuanxiong root extract is extracted using supercritical CO2, leaving no organic solvent residue and effectively retaining fat-soluble active ingredients, ensuring the maximum efficacy of each component.

[0031] 3. The raw materials used in the present invention, including roxburghii fruit, hydrolyzed coffee sunflower, chuanxiong root, and milk thistle seed oil, are all of natural origin and have high purity after scientific extraction and refinement. The auxiliary materials are selected from cosmetic grade raw materials, without irritating ingredients, and are suitable for a variety of skin types. DETAILED DESCRIPTION

[0032] To make the objectives, technical solutions, and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to specific embodiments. However, the following embodiments are merely preferred embodiments of the present invention and are not exhaustive. For the sake of clarity, not all features of the actual embodiments are described.

[0033] Based on the embodiments in the implementation manner, other embodiments obtained by those skilled in the art without making any creative work shall all fall within the protection scope of the present invention.

[0034] In the present invention:

[0035] Rosa roxburghii: The fruit of the plant Rosa roxburghii of the Rosaceae family, commercially available;

[0036] Hydrolyzed Hibiscus esculentus extract: Hydrolyzed Hibiscus esculentus extract, purchased from Shanghai Gefeng Biotechnology Co., Ltd., model: MF210;

[0037] Chuanxiong root: the root of Ligusticum chuanxiong of the genus Ligusticum in the family Apiaceae, commercially available;

[0038] Milk thistle seed oil: Milk thistle (SILYBUM MARIANUM) seed oil was purchased from Shanghai Meiyan Biotechnology Co., Ltd.

[0039] Enzyme preparation: Neutral protease activity 5×10 4 U / g, kiwifruit protease activity 5×10 3 U / g, papain activity 2×104 U / g, aminopeptidase activity 5×10 3 U / g, bromelain enzyme activity 1×10 5 U / g, purchased from Shanghai Xingye Biotechnology Co., Ltd.;

[0040] Preparation of Rosa roxburghii peptide

[0041] Step 1.1. Fresh roxburghii fruit was crushed into a homogenate using a high-speed tissue crusher at 10,000 rpm. Deionized water was added at a solid-liquid ratio of 1:5 g / mL. The pH was adjusted to 4.5-5.0 with 1 mol / L NaOH. The mixture was stirred in a 50°C water bath for 30 min. The precipitate was collected by centrifugation at 4,000 rpm for 15 min. The precipitate was washed with deionized water and the above method was repeated twice to obtain a crude roxburghii protein extract.

[0042] Step 1.2. The crude protein extract of sea buckthorn was used as a substrate for a two-step enzymatic hydrolysis method. First, 5% (by substrate mass) of a composite enzyme 1 consisting of a neutral protease, kiwi protease and papain in a mass ratio of 1:1:1 was added to the substrate. The enzyme was hydrolyzed at 45-55°C and pH 6.5-7.0 for 3 hours. After the enzymatic hydrolysis was completed, the enzyme was inactivated in a water bath at 90°C for 15 minutes. The inactivated enzyme mixture was used as a substrate, and 4% (by substrate mass) of a composite enzyme 2 consisting of an aminopeptidase and bromelain in a mass ratio of 1:1 was added. The enzyme was hydrolyzed at 45-55°C and pH 6.5-7.0 for 4 hours. After the enzymatic hydrolysis was completed, the enzyme was inactivated in a water bath at 90°C for 15 minutes, and the supernatant was collected by centrifugation at 6000 r / min for 20 minutes.

[0043] Step 1.3. The supernatant was ultrafiltered through an ultrafiltration membrane with a molecular weight cutoff of 3000Da, and the permeate was further refined with an ultrafiltration membrane with a molecular weight cutoff of 500Da to obtain a retentate. 200-mesh activated carbon, 2% of the mass of the retentate, was added to the retentate. After stirring at 50°C for 30 minutes, the activated carbon was removed by filtration with a 0.22μm microfiltration membrane to decolorize and deodorize, and then concentrated under reduced pressure and freeze-dried to obtain the roxburghii peptide.

[0044] Preparation of Chuanxiong root extract

[0045] Step 2.1. Grind the dried Chuanxiong root in a grinder and pass it through a 60-mesh sieve to obtain Chuanxiong root powder.

[0046] Step 2.2. The Chuanxiong root powder was evenly charged into a supercritical CO2 extraction vessel. The extraction parameters were set as follows: extraction vessel pressure of 20 MPa, extraction temperature of 35°C, CO2 flow rate of 16 L / h, and extraction time of 1.5 h. After the extraction step, the supercritical CO2 fluid containing the extract entered a separation vessel. The separation parameters were set as follows: pressure of 8 MPa, temperature of 50°C, and separation time of 2 h to obtain an extract.

[0047] Step 2.3. Filter the extract through a 0.22 μm microfiltration membrane to obtain the Chuanxiong root extract.

[0048] Preparation of anti-aging composition

[0049] Weigh the raw materials according to the mass percentages in Table 1 and mix them evenly to obtain an anti-aging composition;

[0050] Table 1 Raw materials mass percentage

[0051]

[0052] Note: “ / ” in the table means no additive.

[0053] Performance Testing

[0054] The anti-aging compositions 1-8 obtained above were prepared with deionized water to prepare 10 mg / mL sample test solutions 1-8, and the following performance tests were performed.

[0055] 1. Elastase inhibition test

[0056] Objective: To evaluate the anti-aging potential of anti-aging compositions 1-8 by measuring their inhibition rate on elastase. Overactivation of elastase can lead to degradation of skin elastic fibers, and inhibiting the activity of this enzyme is one of the important indicators for measuring the efficacy of anti-aging compositions.

[0057] Reagents: BR-grade porcine pancreatic elastase, diluted to 0.5 U / mL with 0.05 mol / L Tris-HCl buffer (pH 8.0) before use; 98% pure N-succinyl-L-alanyl-L-alanyl-L-alanine, dissolved in 0.05 mol / L Tris-HCl buffer (pH 8.0) to prepare a 1 mmol / L substrate solution, prepared immediately before use; 98% pure epigallocatechin gallate (EGCG); 0.05 mol / L Tris-HCl buffer (pH 8.0); and deionized water.

[0058] Test methods

[0059] (1) Treatment of control and test samples

[0060] Sample group: sample test solution 1-8;

[0061] Positive control (EGCG): Dilute with pure water to a positive control concentration of 1 mg / mL;

[0062] Negative control: deionized water.

[0063] (2) Test operation steps

[0064] Set up sample group, sample background group, solvent group and solvent background group. Each group needs to set up 3 parallels. Add different reagent solutions into the 96-well plate respectively, as follows:

[0065] Reagent (μL) Sample group / positive control group Sample background group / positive control background group Solvent group Solvent background group Sample test solution / positive control 20 20 / / Enzyme solution 20 / 20 / Substrate solution 160 160 160 160 Tris-HCl buffer / 20 / 20 Negative control / / 20 20

[0066] After sample addition, gently mix the 96-well plate on a vortex mixer for 30 seconds to avoid creating bubbles. Incubate the 96-well plate in a 25°C incubator for 15 minutes, avoiding exposure to strong light. Immediately after incubation, place the 96-well plate in a microplate reader and measure the absorbance of each well at 410 nm. Record the results. Measure each well three times, and take the average value as the final absorbance value for that well.

[0067] (3) The calculation formula is shown in formula (1)

[0068] Inhibition rate / % = [1-(CD) / (AB)] × 100% (1) where:

[0069] A——average absorbance of solvent group;

[0070] B——average absorbance of solvent background group;

[0071] C——average absorbance of the sample group (or positive control group);

[0072] D——The average absorbance of the sample background group (or positive control background group).

[0073] (4) Test results

[0074] The elastase inhibition test results of the positive control and sample test solutions 1-8 are shown in Table 2.

[0075] Table 2 Elastase inhibition rate

[0076] Group Inhibition rate / % Positive control (EGCG) 76.82±3.15 Sample test solution 1 (corresponding to composition 1) 65.36±2.87 Sample test solution 2 (corresponding to composition 2) 71.70±1.95 Sample test solution 3 (corresponding to composition 3) 72.46±2.43 Sample test solution 4 (corresponding to composition 4) 31.27±3.62*** Sample test solution 5 (corresponding to composition 5) 18.50±2.85*** Sample test solution 6 (corresponding to composition 6) 14.15±2.10*** Sample test solution 7 (corresponding to composition 7) 9.10±1.50*** Sample test solution 8 (corresponding to composition 8) 0.65±0.02***

[0077] Note: “*” indicates significant difference compared with the sample solution 2, P < 0.05, “**” indicates P < 0.01, and “***” indicates P < 0.001.

[0078] Result analysis:

[0079] Overactivation of elastase is a key mechanism that leads to the degradation of skin elastic fibers and triggers skin aging. Therefore, elastase inhibition is a key indicator for evaluating the efficacy of anti-aging compositions. In this study, epigallocatechin gallate (EGCG) was used as a positive control, demonstrating a 76.82% inhibition rate, indicating strong elastase inhibitory activity.

[0080] Judging from the sample test results, compositions 1-3 (containing roxburghii peptide, hydrolyzed coffee sunflower extract, chuanxiong root extract and milk thistle seed oil) all showed significant elastase inhibition effects, with inhibition rates of 65.36%, 71.70% and 72.46%, respectively. Among them, the inhibition rates of compositions 2 and 3 were close to the positive control level, indicating that these two formulas can effectively inhibit elastase activity and have good potential to protect skin elastic fibers.

[0081] The inhibition rates of the control groups containing only a single active ingredient (samples 4-7) and the control group containing only the basic ingredient (sample 8) were significantly reduced. Statistical analysis showed that there were extremely significant differences (P < 0.001) between the above control groups and composition 2, proving that roxburghii peptide, hydrolyzed coffee sunflower extract, Chuanxiong root extract, and milk thistle seed oil played a synergistic role in inhibiting elastase activity. The absence of a single component or multiple components would lead to a significant decrease in the inhibitory effect.

[0082] 2. Antioxidant test

[0083] DPPH scavenging ability assay

[0084] Weigh 7.8 mg of DPPH powder and add 100 mL of anhydrous ethanol to prepare a 0.2 mmol / L DPPH anhydrous ethanol mixed solution. Accurately pipette 200 μL of the DPPH anhydrous ethanol mixed solution and 200 μL of the sample solution to be tested, shake and mix, and then place it in the dark at room temperature for 30 minutes. Accurately pipette 200 μL of anhydrous ethanol solution and 200 μL of the sample solution to be tested, shake and mix. Add 200 μL of deionized water and 200 μL of the DPPH anhydrous ethanol mixed solution, shake and mix. Use vitamin C (VC) with a concentration of 0.01 mg / mL as a positive control. Measure its OD value at a wavelength of 517 nm in an enzyme reader, and calculate the clearance rate according to formula (2).

[0085] Clearance (%) = [1-(A1-A2) / A3] × 100% (2)

[0086] Where:

[0087] A1 is the OD value of 200 μL of the sample solution and 200 μL of the DPPH anhydrous ethanol mixture;

[0088] A2 is the OD value of 200 μL of anhydrous ethanol and the sample solution to be tested;

[0089] A3 is the OD value of 200 μL of DPPH anhydrous ethanol mixture and deionized water.

[0090] Table 2 Antioxidant test results

[0091] Group DPPH clearance rate / % Positive control (VC) 67.24±4.11 Sample test solution 1 (corresponding to composition 1) 78.95±3.20 Sample test solution 2 (corresponding to composition 2) 83.63±2.15 Sample test solution 3 (corresponding to composition 3) 81.15±2.80 Sample test solution 4 (corresponding to composition 4) 19.60±4.05*** Sample test solution 5 (corresponding to composition 5) 25.23±3.15*** Sample test solution 6 (corresponding to composition 6) 16.34±2.65*** Sample test solution 7 (corresponding to composition 7) 15.80±2.05*** Sample test solution 8 (corresponding to composition 8) 0.77±0.20***

[0092] Note: “*” indicates significant difference compared with the sample solution 2, P < 0.05, “**” indicates P < 0.01, and “***” indicates P < 0.001.

[0093] Result Analysis

[0094] Skin aging results from an imbalance between oxidative stress and the antioxidant system. Accumulation of free radicals can trigger oxidative damage to lipids and proteins. DPPH free radical scavenging capacity is a classic indicator for evaluating a composition's antioxidant activity. In this study, vitamin C (VC) was used as a positive control, and its DPPH scavenging rate was 67.24±4.11%, indicating strong antioxidant capacity.

[0095] The test results showed that the DPPH scavenging rates of compositions 1-3 were significantly higher than that of the positive control, among which composition 2 had the highest scavenging rate of 83.63%, while compositions 1 and 3 were 78.95% and 81.15%, respectively, indicating that these three compositions have excellent free radical scavenging ability and can effectively reduce the damage of oxidative stress to the skin.

[0096] The DPPH scavenging rate of sample 4 was 19.60%, sample 5 was 25.23%, sample 6 was 16.34%, sample 7 was 15.80%, and sample 8 was only 0.77%, all of which were much lower than compositions 1-3. Statistical analysis showed that there were extremely significant differences between the control group and composition 2 (P < 0.001), verifying the synergistic antioxidant effect of roxburghii peptide, hydrolyzed coffee sunflower extract, Chuanxiong root extract and milk thistle seed oil - when multiple ingredients work together, they can significantly enhance the free radical scavenging ability, while the absence of a single or multiple active ingredients will lead to a sharp decrease in antioxidant activity.

[0097] From the perspective of active ingredient characteristics, sea buckthorn peptides are obtained through two-step enzymatic hydrolysis to obtain small molecule peptides, which retain rich antioxidant amino acid residues. Chuanxiong root extract is prepared by supercritical CO2 extraction technology, which can effectively retain fat-soluble antioxidant components. The hydrolyzed coffee sunflower extract is rich in vitamin C and unsaturated fatty acids. Milk thistle seed oil itself is rich in unsaturated fatty acids and fat-soluble antioxidants. The four can synergize to enhance antioxidant efficacy from different dimensions.

[0098] In summary, the anti-aging compositions provided herein demonstrate excellent performance in two key anti-aging pathways: inhibiting elastase activity and scavenging free radicals, through the synergistic effects of roxburghii peptides, hydrolyzed amomum villosum extract, ligusticum chuanxiong root extract, and milk thistle seed oil. Compositions 1-3 effectively protect skin elastic fibers from elastase degradation and mitigate oxidative stress damage through strong antioxidant activity, demonstrating promising potential for anti-aging applications.

[0099] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art will be able to modify the technical solutions described in the aforementioned embodiments or substitute equivalents for some of the technical features. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the present invention.

Claims

1. An anti-aging composition, characterized in that The composition contains roxburghii peptide, hydrolyzed amla extract, ligusticum chuanxiong root extract, silybum marianum seed oil, and auxiliary materials and solvents acceptable in cosmetics.

2. The anti-aging composition according to claim 1, characterized in that The composition comprises the following components by mass percentage:

3. The anti-aging composition according to claim 1 or 2, characterized in that The preparation method of the roxburghii peptide comprises the following steps: Step 1.

1. Crush the roxburghii fruit into a homogenate, add deionized water at a solid-liquid ratio of 1:(5-10) g / mL, adjust the pH to 4.5-5.0, stir in a 45-55°C water bath for 25-35 minutes, then centrifuge to collect the precipitate, and wash 2-3 times with deionized water to obtain a crude roxburghii protein extract; Step 1.

2. The crude protein extract of the roxburghii was used as a substrate for a two-step enzymatic hydrolysis method. First, 3-5% of a composite enzyme 1 consisting of a neutral protease, kiwi protease, and papain was added to the substrate by weight. The enzyme was hydrolyzed at 45-55°C and pH 6.5-7.0 for 2-4 hours. After the hydrolysis was completed, the enzyme was inactivated in a water bath at 80-90°C for 10-15 minutes. The inactivated enzyme mixture was used as a substrate. 3-5% of a composite enzyme 2 consisting of an aminopeptidase and bromelain was added by weight of the substrate. The enzyme was hydrolyzed at 45-55°C and pH 6.5-7.0 for 2-4 hours. After the hydrolysis was completed, the enzyme was inactivated in a water bath at 80-90°C for 10-15 minutes. The supernatant was collected by centrifugation. Step 1.

3. The supernatant was ultrafiltered through an ultrafiltration membrane with a molecular weight cutoff of 3000Da, and the permeate was further ultrafiltered through a 500Da molecular weight cutoff ultrafiltration membrane to obtain a retentate. 1%-2% activated carbon was added to the retentate, and the mixture was stirred at 45-50°C for 20-30 minutes. The activated carbon was removed by filtration and then concentrated under reduced pressure and freeze-dried to obtain the roxburghii peptide.

4. The anti-aging composition according to claim 3, characterized in that The neutral protease activity was 5×10 4 U / g, kiwifruit protease activity 5×10 3 U / g, papain activity 2×10 4 U / g, complex enzyme 1 composed of neutral protease, kiwi protease and papain in a mass ratio of 1:1:

1.

5. The anti-aging composition according to claim 3, characterized in that The aminopeptidase activity was 5×10 3 U / g, bromelain enzyme activity 1×10 5 U / g, complex enzyme 2 composed of aminopeptidase and bromelain in a mass ratio of 1:

1.

6. The anti-aging composition according to claim 1 or 2, characterized in that The preparation method of the Chuanxiong root extract comprises the following steps: Step 2.

1. Grind the dried Chuanxiong root through a 60-mesh sieve to obtain Chuanxiong root powder. Step 2.

2. Load the Chuanxiong root powder into a supercritical CO2 extraction vessel for extraction and separation to obtain an extract; Step 2.

3. Filter the extract through a 0.22 μm microfiltration membrane to obtain the Chuanxiong root extract.

7. The anti-aging composition according to claim 6, characterized in that The extraction parameters in step 2.2 are: extraction kettle pressure 18-22 MPa, extraction temperature 30-40°C, CO2 flow rate 15-18 L / h, extraction time 1-1.5 h; the separation parameters are: pressure 6-10 MPa, temperature 45-55°C, separation time 2 h, to obtain an extract.

8. Use of the anti-aging composition according to any one of claims 1 to 7 in the preparation of skin care products with antioxidant, anti-aging, whitening, firming and / or anti-wrinkle effects.

9. The use according to claim 8, characterized in that The anti-aging composition is added in an amount of 5-10 wt % in the skin care product.

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