Anti-aging and anti-wrinkle composition containing various polypeptides as well as preparation method and application of anti-aging and anti-wrinkle composition

Abstract

The invention discloses an anti-aging and anti-wrinkle composition containing various polypeptides as well as a preparation method and application of the anti-aging and anti-wrinkle composition. According to the anti-aging and anti-wrinkle composition, various polypeptide components are compounded, a synergistic interaction system of the polypeptides is formed, acetyl hexapeptide-8 and snake venom-like peptide are taken as cores, and a dual inhibition pathway is established aiming at dynamic wrinkles; hexapeptide-9 and palmitoyl pentapeptide-4 are compounded to repair a matrix, and regeneration of corium layer structural protein is promoted; with the assistance of mu-conopeptide, palmitoyl tripeptide-1 and palmitoyl tripeptide-5, static wrinkle improvement and skin density improvement are enhanced; and finally, acetyl tetrapeptide-9 is assisted to activate elastin and optimize the contour supporting force, so that the synergistic effect of three effects of resisting wrinkles, tightening and repairing is realized, and the composition has more excellent inhibition rate of elastase and excellent free radical scavenging capacity, and achieves a remarkable antioxidant effect and an excellent anti-aging and anti-wrinkle effect.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to an anti-aging and anti-wrinkle composition containing multiple polypeptides, and a preparation method and application thereof. Background Art

[0002] The main layer of the skin is the dermis, which is primarily composed of fibroblasts and matrix components. Fibroblasts produce glycosaminoglycans such as collagen and hyaluronic acid, forming connective tissue and playing a vital role in the skin. Decreased fibroblast function due to aging or other factors, such as ultraviolet radiation, can lead to a decrease and denaturation of matrix components such as collagen and hyaluronic acid. Oxidative stress, such as ultraviolet radiation, damages the skin, causing it to become rough and trigger other adverse reactions. This decrease in matrix components and oxidative stress accelerate skin aging, resulting in wrinkles, spots, a dull appearance, a loss of smooth texture, and decreased elasticity, among other signs of aging. The most prominent characteristic of skin aging is the increase in facial wrinkles, which is both genetically pre-programmed and influenced by environmental factors. Physiologically, skin aging manifests itself in a decrease in collagen and elastin and a disordered distribution of skin lipids.

[0003] Peptides are another important direction for skin anti-aging. Many substances with antioxidant properties in organisms belong to proteins and peptides. Since proteins and peptides have excellent emulsifying properties and can mediate at the oil-water interface, they are of great significance in scavenging excess free radicals in the body and inhibiting membrane lipid peroxidation. However, the absorption degree of anti-aging skin care products by skin of different skin types and different age stages is not the same. The anti-aging products in the existing technology are also varied. Most anti-aging skin care products have a single effect and a large molecular weight, which makes them difficult to be absorbed and utilized by the skin, resulting in poor anti-aging effects. Summary of the Invention

[0004] Based on this, in order to solve the problem that the existing anti-aging skin care products have a single effect and a large molecular weight, which makes them difficult to be absorbed and utilized by the skin, resulting in poor anti-aging effect, the present invention provides an anti-aging and anti-wrinkle composition containing multiple polypeptides, and its preparation method and application. The specific technical solution is as follows:

[0005] An anti-aging and anti-wrinkle composition containing multiple polypeptides, the anti-aging and anti-wrinkle composition comprising the following components in percentage by mass:

[0006] Acetyl hexapeptide-8: 0.05-0.2%, snake venom peptide: 0.01-0.1%, hexapeptide-9: 0.01-0.2%, arginine polypeptide: 0.001-0.1%, μ-cono peptide: 0.01-0.1%, acetyl tetrapeptide-9: 0.01-0.1%, palmitoyl pentapeptide-4: 0.01-0.1%, palmitoyl tripeptide-1: 0.01-0.1%, palmitoyl tripeptide-5: 0.01-0.1%, butylene glycol: 15-40%, glycerin: 5-30%, 1,2-hexanediol: 0.5-5%, ethylhexylglycerin: 0.01-0.1%, steareth-20: 1-5%, purified water: balance

[0007] Furthermore, the arginine polypeptide can be replaced by a lysine polypeptide.

[0008] Furthermore, the anti-aging and anti-wrinkle composition comprises the following components in percentage by mass:

[0009] Acetyl Hexapeptide-8: 0.10%, Snake Venom-like Peptide: 0.05%, Hexapeptide-9: 0.05%, Arginine / Lysine Peptide: 0.005%, μ-Conopeptide: 0.05%, Acetyl Tetrapeptide-9: 0.012%, Palmitoyl Pentapeptide-4: 0.012%, Palmitoyl Tripeptide-1: 0.012%, Palmitoyl Tripeptide-5: 0.012%, Butylene Glycol: 30%, Glycerin: 20%, 1,2-Hexanediol: 2.0%, Ethylhexylglycerin: 0.05%, Steareth-20: 2.0%, Purified Water: 45.697%.

[0010] Furthermore, the anti-aging and anti-wrinkle composition comprises the following components in percentage by mass:

[0011] Acetyl Hexapeptide-8: 0.05%, Snake Venom-like Peptide: 0.10%, Hexapeptide-9: 0.10%, Arginine / Lysine Peptide: 0.005%, μ-Conopeptide: 0.02%, Acetyl Tetrapeptide-9: 0.02%, Palmitoyl Pentapeptide-4: 0.02%, Palmitoyl Tripeptide-1: 0.02%, Palmitoyl Tripeptide-5: 0.02%, Butylene Glycol: 20%, Glycerin: 30%, 1,2-Hexanediol: 1.0%, Ethylhexylglycerin: 0.15%, Steareth-20: 2.0%, Purified Water: Balance

[0012] Furthermore, the anti-aging and anti-wrinkle composition comprises the following components in percentage by mass:

[0013] Acetyl Hexapeptide-8: 0.2%, Snake Venom-like Peptide: 0.10%, Hexapeptide-9: 0.05%, Arginine / Lysine Peptide: 0.01%, μ-Conopeptide: 0.05%, Acetyl Tetrapeptide-9: 0.012%, Palmitoyl Pentapeptide-4: 0.012%, Palmitoyl Tripeptide-1: 0.012%, Palmitoyl Tripeptide-5: 0.012%, Butylene Glycol: 40%, Glycerin: 20%, 1,2-Hexanediol: 2.0%, Ethylhexylglycerin: 0.05%, Steareth-20: 2.0%, Purified Water: Balance

[0014] In addition, the present invention also provides a method for preparing an anti-aging and anti-wrinkle composition, the preparation method comprising the following steps:

[0015] The purified water was heated to 40° C. to 45° C., and then butanediol and glycerol were added, and the mixture was stirred at a speed of 300 r / min to 500 r / min for 10 minutes. Acetyl hexapeptide-8, snake venom peptide, hexapeptide-9, arginine peptide / lysine peptide, μ-conopeptide, acetyl tetrapeptide-9, palmitoyl pentapeptide-4, palmitoyl tripeptide-1, and palmitoyl tripeptide-5 were added in sequence, and the mixture was stirred for 15 minutes to 30 minutes to obtain a mixture A.

[0016] Add steareth-20 to the mixture A, raise the temperature to 60° C. to 75° C., and homogenize at a speed of 1000 r / min to 2000 r / min for 3 min to 5 min to obtain a mixture B;

[0017] 1,2-Hexanediol and ethylhexylglycerin are added to the mixture B, and the mixture is homogenized at a rotation speed of 1000 r / min to 1500 r / min for 1 to 5 minutes, and then allowed to stand for degassing to obtain an anti-aging and anti-wrinkle composition.

[0018] In addition, the present invention also provides an application of the anti-aging and anti-wrinkle composition, which is the application of the anti-aging and anti-wrinkle composition in the preparation of anti-aging and anti-wrinkle skin care products.

[0019] Furthermore, the anti-aging and anti-wrinkle skin care product comprises the anti-aging and anti-wrinkle composition and chemically acceptable auxiliary materials.

[0020] Furthermore, the auxiliary material is at least one of acetate, hydrochloride, citrate, and trifluoroacetate.

[0021] Furthermore, the dosage form of the anti-aging and anti-wrinkle skin care product is at least one of an aqueous solution, an emulsion, a gel, a cream, a powder, an oil, a spray and a mask.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] 1. After the polypeptides are compounded according to a specific ratio, the present invention inhibits skin aging and wrinkle formation through multiple mechanisms of action, produces a significant synergistic effect in terms of antioxidant efficacy, and achieves a significant anti-aging effect.

[0024] 2. The present invention adds acetyl hexapeptide-8, which makes it impossible for synaptic vesicles to effectively release acetylcholine, reduces the contraction signal of nerves to muscles, thereby relieving muscle contraction and having a very obvious inhibitory effect on dynamic lines; snake venom peptides can specifically bind to nicotinic acetylcholine receptors on muscle cells, preventing acetylcholine from binding to the receptors; hexapeptide-9 promotes the synthesis of extracellular matrix and inhibits the activity of metalloproteinases to play an anti-aging, repair skin barrier and improve wrinkles; μ-cono peptide can inhibit muscle cell sodium channels (Nav1.4), block action potentials, and prevent nerve signals from being effectively transmitted to muscles, so that facial muscles relax and reduce dynamic lines. For wrinkles (such as crow's feet and forehead wrinkles), the combination of μ-conopeptide and hexapeptide-9 can produce dual anti-aging effects of anti-wrinkle and collagen regeneration; acetyl tetrapeptide-9 can improve microcirculation and enhance vascular elasticity; palmitoyl pentapeptide-4 can regulate the key growth factors of extracellular matrix synthesis; palmitoyl tripeptide-1 can stimulate fibroblast proliferation and promote the gene expression of type I collagen (COL1A1), type III collagen (COL3A1) and elastin (ELN); palmitoyl tripeptide-5 can strongly promote the synthesis of collagen and elastin by specifically activating the TGF-β pathway, while inhibiting collagen degradation, thereby achieving deep anti-wrinkle and skin structure reconstruction.

[0025] 3. The present invention forms a synergistic enhancement system of polypeptides by compounding multiple polypeptide ingredients, with acetyl hexapeptide-8 and snake venom peptide as the core, to establish a dual inhibition pathway for dynamic lines; compound hexapeptide-9 and palmitoyl pentapeptide-4 to repair the matrix and promote the regeneration of dermal structural proteins; supplemented with μ-cono peptide, palmitoyl tripeptide-1 and palmitoyl tripeptide-5, it strengthens the improvement of static lines and increases skin density; finally supplemented with acetyl tetrapeptide-9 to activate elastin and optimize contour support, achieving the triple synergistic effects of anti-wrinkle, firming and repair, with a better inhibition rate of elastase, excellent free radical scavenging ability, and significant antioxidant effect, achieving excellent anti-aging and anti-wrinkle effects.

[0026] 4. Human skin tests demonstrated that the anti-aging and anti-wrinkle composition of the present invention significantly improved skin after 30 days of continuous use. This indicates that the anti-aging and anti-wrinkle composition of the present invention is excellent in increasing the moisture content of the stratum corneum, improving skin elasticity, and reducing fine lines, thereby achieving the purpose of anti-aging and anti-wrinkle. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with its embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and do not limit the scope of protection of the present invention.

[0028] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this invention pertains. The terms used herein in the specification of the present invention are for the purpose of describing specific embodiments only and are not intended to limit the present invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0029] In one embodiment of the present invention, an anti-aging and anti-wrinkle composition containing multiple polypeptides comprises the following components in percentage by weight:

[0030] Acetyl Hexapeptide-8: 0.05-0.2%, Snake Venom-like Peptide: 0.01-0.1%, Hexapeptide-9: 0.01-0.2%, Arginine / Lysine Peptide: 0.001-0.1%, μ-Conopeptide: 0.01-0.1%, Acetyl Tetrapeptide-9: 0.01-0.1%, Palmitoyl Pentapeptide-4: 0.01-0.1%, Palmitoyl Tripeptide-1: 0.01-0.1%, Palmitoyl Tripeptide-5: 0.01-0.1%, Butylene Glycol: 15-40%, Glycerin: 5-30%, 1,2-Hexanediol: 0.5-5%, Ethylhexylglycerin: 0.01-0.1%, Steareth-20: 1-5%, Purified Water: Balance

[0031] The invention compounds the drug by adding acetyl hexapeptide-8, snake venom peptide, hexapeptide-9, arginine polypeptide, μ-conopeptide, acetyl tetrapeptide-9, palmitoyl pentapeptide-4, palmitoyl tripeptide-1 and palmitoyl tripeptide-5.

[0032] Among them, acetyl hexapeptide-8 targets the SNARE complex by mimicking the N-terminal sequence of SNAP-25. It competitively binds to the Syntaxin protein and interferes with the complete assembly of the SNARE complex. As a result, the synaptic vesicles that cause wrinkles cannot effectively release acetylcholine, reducing the contraction signal of the nerve to the muscle, thereby relieving muscle contraction and having a very significant inhibitory effect on dynamic lines.

[0033] Snake venom peptides specifically bind to nicotinic acetylcholine receptors (nAChRs) on muscle cells, preventing acetylcholine (ACh) from binding to the receptors. This prevents nerve signals from reaching the muscles, reducing muscle contraction and thus alleviating dynamic wrinkles. Snake venom peptides reduce repetitive muscle movement and the frequency of skin folding, thereby delaying the conversion of dynamic lines into static lines.

[0034] Hexapeptide-9's main targets are fibroblasts and MMPs. It promotes the synthesis of the extracellular matrix (ECM) and inhibits the activity of metalloproteinases (MMPs) to play an anti-aging, skin barrier repair and wrinkle improvement role. Based on its target, hexapeptide-9 can repair the dermis in the long term, promote collagen synthesis, and resist photoaging. It achieves anti-aging through a dual pathway of "rebuilding dermal structure + protecting collagen", and is suitable for improving skin sagging and static wrinkles caused by photoaging or natural aging.

[0035] Arginine / lysine peptides can regulate key growth factors in the synthesis of the extracellular matrix (ECM). Palmitoyl tripeptide-1 stimulates fibroblast proliferation by activating the TGF-β / Smad signaling pathway and promotes gene expression of type I collagen (COL1A1), type III collagen (COL3A1), and elastin (ELN).

[0036] μ-Conopeptide can inhibit the sodium channel (Nav1.4) in muscle cells, blocking action potentials and preventing nerve signals from being effectively transmitted to the muscles. This relaxes facial muscles and reduces dynamic wrinkles (such as crow's feet and forehead wrinkles). The combination of μ-Conopeptide and Hexapeptide-9 can produce a dual anti-aging effect, including anti-wrinkle and collagen regeneration.

[0037] Acetyl tetrapeptide-9 is an active peptide commonly used for anti-wrinkle and anti-edema. Its core function is to improve microcirculation and enhance vascular elasticity. When used in combination with traditional neurotransmitter inhibitory peptides (such as acetyl hexapeptide-8 and conopeptide), acetyl tetrapeptide-9 can alleviate the stimulation of neurotransmitter inhibitory peptides on skin microvessels, promote metabolism and reduce inflammation, making the skin firmer.

[0038] Palmitoyl tripeptide-1 can upregulate the expression of hyaluronic acid synthase (HAS2), increase the production of endogenous hyaluronic acid in the skin, and enhance moisturizing ability and skin plumpness. Palmitoyl tripeptide-5 and palmitoyl tripeptide-4 can strongly promote the synthesis of collagen and elastin by specifically activating the TGF-β pathway, while inhibiting collagen degradation, thereby achieving deep anti-wrinkle and skin structure reconstruction. With the addition of palmitoyl tripeptide-1 and palmitoyl tripeptide-5, in the early stage of the aqueous solution system of multiple polypeptides, all polypeptides can be cross-linked through some physical and chemical bonds (such as hydrogen bonds). Through the action of the palmitoyl group itself, the permeability of the skin is greatly enhanced. Compared with the individual polypeptide components, the corresponding transdermal absorption rate of the composition will be greatly improved. At the same time, palmitoyl tripeptide-1 and palmitoyl tripeptide-5 can also promote the formation of collagen, increase skin elasticity, and produce anti-aging effects. Based on the synergistic peptide enhancement system, with acetyl hexapeptide-8 and snake venom peptide as the core, a dual inhibition pathway is established for dynamic lines; hexapeptide-9 and palmitoyl pentapeptide-4 are compounded to repair the matrix and promote the regeneration of dermal structural proteins; supplemented with conoside peptide and palmitoyl tripeptide-1 / 5, it strengthens the improvement of static lines and increases skin density; finally, supplemented with acetyl tetrapeptide-9 to activate elastin and optimize contour support, achieving the synergistic triple effects of anti-wrinkle, firming and repair.

[0039] Therefore, the anti-aging and anti-wrinkle composition containing multiple peptides can comprehensively inhibit all factors that cause skin aging through different mechanisms of action at different targets. It can simultaneously solve the skin aging caused by dynamic and static lines, and simultaneously improve microcirculation in the deep layers of the skin, enhance vascular elasticity, and improve skin firmness. Most importantly, the addition of acetyl tetrapeptide-9 can improve the stimulation of active substances on some sensitive skin inflammation, and simultaneously achieve a soothing effect.

[0040] In one embodiment, the arginine polypeptide can be replaced by a lysine polypeptide.

[0041] In one embodiment, the anti-aging and anti-wrinkle composition comprises the following ingredients in percentage by weight:

[0042] Acetyl Hexapeptide-8: 0.10%, Snake Venom-like Peptide: 0.05%, Hexapeptide-9: 0.05%, Arginine / Lysine Peptide: 0.005%, μ-Conopeptide: 0.05%, Acetyl Tetrapeptide-9: 0.012%, Palmitoyl Pentapeptide-4: 0.012%, Palmitoyl Tripeptide-1: 0.012%, Palmitoyl Tripeptide-5: 0.012%, Butylene Glycol: 30%, Glycerin: 20%, 1,2-Hexanediol: 2.0%, Ethylhexylglycerin: 0.05%, Steareth-20: 2.0%, Purified Water: Balance

[0043] In one embodiment, the anti-aging and anti-wrinkle composition comprises the following ingredients in percentage by weight:

[0044] Acetyl Hexapeptide-8: 0.05%, Snake Venom-like Peptide: 0.10%, Hexapeptide-9: 0.10%, Arginine / Lysine Peptide: 0.005%, μ-Conopeptide: 0.02%, Acetyl Tetrapeptide-9: 0.02%, Palmitoyl Pentapeptide-4: 0.02%, Palmitoyl Tripeptide-1: 0.02%, Palmitoyl Tripeptide-5: 0.02%, Butylene Glycol: 20%, Glycerin: 30%, 1,2-Hexanediol: 1.0%, Ethylhexylglycerin: 0.15%, Steareth-20: 2.0%, Purified Water: Balance

[0045] In one embodiment, the anti-aging and anti-wrinkle composition comprises the following ingredients in percentage by weight:

[0046] Acetyl Hexapeptide-8: 0.2%, Snake Venom-like Peptide: 0.10%, Hexapeptide-9: 0.05%, Arginine / Lysine Peptide: 0.01%, μ-Conopeptide: 0.05%, Acetyl Tetrapeptide-9: 0.012%, Palmitoyl Pentapeptide-4: 0.012%, Palmitoyl Tripeptide-1: 0.012%, Palmitoyl Tripeptide-5: 0.012%, Butylene Glycol: 40%, Glycerin: 20%, 1,2-Hexanediol: 2.0%, Ethylhexylglycerin: 0.05%, Steareth-20: 2.0%, Purified Water: Balance

[0047] In one embodiment, the snake venom-like peptide is dipeptide diaminobutyrylbenzylamide diacetate.

[0048] In one embodiment, the anti-aging and anti-wrinkle composition may further include a salt acceptable in cosmetics, including but not limited to at least one of acetate, hydrochloride, citrate, and trifluoroacetate.

[0049] In addition, the present invention also provides a method for preparing an anti-aging and anti-wrinkle composition, the preparation method comprising the following steps:

[0050] The purified water was heated to 40° C. to 45° C., and then butanediol and glycerol were added, and the mixture was stirred at a speed of 300 r / min to 500 r / min for 10 minutes. Acetyl hexapeptide-8, snake venom peptide, hexapeptide-9, arginine peptide / lysine peptide, μ-conopeptide, acetyl tetrapeptide-9, palmitoyl pentapeptide-4, palmitoyl tripeptide-1, and palmitoyl tripeptide-5 were added in sequence, and the mixture was stirred for 15 minutes to 30 minutes to obtain a mixture A.

[0051] Add steareth-20 to the mixture A, raise the temperature to 60° C. to 75° C., and homogenize at a speed of 1000 r / min to 2000 r / min for 3 min to 5 min to obtain a mixture B;

[0052] 1,2-Hexanediol and ethylhexylglycerin are added to the mixture B, and the mixture is homogenized at a rotation speed of 1000 r / min to 1500 r / min for 1 to 5 minutes, and then allowed to stand for degassing to obtain an anti-aging and anti-wrinkle composition.

[0053] In one embodiment, the pretreatment is: placing in ammonium sulfate with a mass percentage concentration of 1% to 5% for salting out for 20 to 30 hours, controlling the salting out temperature at 5-10° C., and dialyzing with purified water.

[0054] In one embodiment, the standing degassing time is 30 minutes to 60 minutes.

[0055] In addition, the present invention also provides an application of the anti-aging and anti-wrinkle composition, which is the application of the anti-aging and anti-wrinkle composition in the preparation of anti-aging and anti-wrinkle skin care products.

[0056] In one embodiment, the anti-aging and anti-wrinkle skin care product comprises the anti-aging and anti-wrinkle composition and chemically acceptable excipients.

[0057] In one embodiment, the excipient is at least one of acetate, hydrochloride, citrate, and trifluoroacetate.

[0058] In one embodiment, the dosage form of the anti-aging and anti-wrinkle skin care product is at least one of an aqueous solution, an emulsion, a gel, a cream, a powder, an oil, a spray, and a mask.

[0059] The above solution, by compounding multiple polypeptide ingredients and forming a synergistic enhancement system of polypeptides, can be effectively absorbed by the skin, resulting in significant anti-aging and anti-wrinkle effects.

[0060] The embodiments of the present invention will be described in detail below with reference to specific examples.

[0061] Example 1:

[0062] An anti-aging and anti-wrinkle composition comprising the following ingredients in percentage by mass:

[0063] Acetyl Hexapeptide-8: 0.10%, Dipeptide Diaminobutyroyl Benzylamide Diacetate: 0.05%, Hexapeptide-9: 0.05%, Arginine Peptide: 0.005%, μ-Conopeptide: 0.05%, Acetyl Tetrapeptide-9: 0.012%, Palmitoyl Pentapeptide-4: 0.012%, Palmitoyl Tripeptide-1: 0.012%, Palmitoyl Tripeptide-5: 0.012%, Butylene Glycol: 30%, Glycerin: 20%, 1,2-Hexanediol: 2.0%, Ethylhexylglycerin: 0.05%, Steareth-20: 2.0%, Purified Water: 45.647%;

[0064] A method for preparing an anti-aging and anti-wrinkle composition comprises the following steps:

[0065] Purified water was heated to 40° C., and then butanediol and glycerol were added, and the mixture was stirred at a speed of 300 r / min for 10 minutes. Acetyl hexapeptide-8, dipeptide diaminobutyryl benzylamide diacetate, hexapeptide-9, arginine peptide / lysine peptide, μ-conopeptide, acetyl tetrapeptide-9, palmitoyl pentapeptide-4, palmitoyl tripeptide-1, and palmitoyl tripeptide-5 were added in sequence, and the mixture was stirred for 25 minutes to obtain a mixture A.

[0066] Adding steareth-20 to the mixture A, heating to 65° C., homogenizing at 1000 r / min for 5 minutes, then placing in 3% by mass ammonium sulfate for salting out for 25 hours at a salting-out temperature of 5° C., and dialyzing with purified water to obtain a mixture B;

[0067] 1,2-Hexanediol and ethylhexylglycerin were added to the mixture B, and the mixture was homogenized at a speed of 1200 r / min for 3 minutes, and then allowed to stand for degassing for 45 minutes to obtain an anti-aging and anti-wrinkle composition containing multiple polypeptides.

[0068] Example 2:

[0069] An anti-aging and anti-wrinkle composition comprising the following ingredients in percentage by mass:

[0070] Acetyl Hexapeptide-8: 0.05%, Dipeptide Diaminobutyroyl Benzylamide Diacetate: 0.10%, Hexapeptide-9: 0.10%, Arginine Peptide: 0.005%, μ-Conopeptide: 0.02%, Acetyl Tetrapeptide-9: 0.02%, Palmitoyl Pentapeptide-4: 0.02%, Palmitoyl Tripeptide-1: 0.02%, Palmitoyl Tripeptide-5: 0.02%, Butylene Glycol: 20%, Glycerin: 30%, 1,2-Hexanediol: 1.0%, Ethylhexylglycerin: 0.15%, Steareth-20: 2.0%, Purified Water: 46.495%;

[0071] A method for preparing an anti-aging and anti-wrinkle composition comprises the following steps:

[0072] Purified water was heated to 45° C., and then butanediol and glycerol were added, and the mixture was stirred at a speed of 500 r / min for 10 minutes. Acetyl hexapeptide-8, dipeptide diaminobutyryl benzylamide diacetate, hexapeptide-9, arginine peptide / lysine peptide, μ-conopeptide, acetyl tetrapeptide-9, palmitoyl pentapeptide-4, palmitoyl tripeptide-1, and palmitoyl tripeptide-5 were added in sequence, and the mixture was stirred for 20 minutes to obtain a mixture A.

[0073] Adding steareth-20 to the mixture A, heating to 65° C., homogenizing at 1500 r / min for 5 minutes, then salting out in 3% by mass ammonium sulfate for 25 hours at a temperature of 5° C., and dialyzing with purified water to obtain a mixture B;

[0074] 1,2-Hexanediol and ethylhexylglycerin were added to the mixture B, and the mixture was homogenized at a speed of 1200 r / min for 3 minutes, and then allowed to stand for degassing for 50 minutes to obtain an anti-aging and anti-wrinkle composition containing multiple polypeptides.

[0075] Example 3:

[0076] An anti-aging and anti-wrinkle composition comprising the following ingredients in percentage by mass:

[0077] Acetyl Hexapeptide-8: 0.2%, Dipeptide Diaminobutyroyl Benzylamide Diacetate: 0.10%, Hexapeptide-9: 0.05%, Lysine Polypeptide: 0.01%, μ-Conopeptide: 0.05%, Acetyl Tetrapeptide-9: 0.012%, Palmitoyl Pentapeptide-4: 0.012%, Palmitoyl Tripeptide-1: 0.012%, Palmitoyl Tripeptide-5: 0.012%, Butylene Glycol: 40%, Glycerin: 20%, 1,2-Hexanediol: 2.0%, Ethylhexylglycerin: 0.05%, Steareth-20: 2.0%, Purified Water: 35.492%;

[0078] A method for preparing an anti-aging and anti-wrinkle composition comprises the following steps:

[0079] Purified water was heated to 45° C., and then butanediol and glycerol were added, and the mixture was stirred at a speed of 500 r / min for 10 minutes. Acetyl hexapeptide-8, dipeptide diaminobutyryl benzylamide diacetate, hexapeptide-9, lysine polypeptide, μ-conopeptide, acetyl tetrapeptide-9, palmitoyl pentapeptide-4, palmitoyl tripeptide-1, and palmitoyl tripeptide-5 were added in sequence, and the mixture was stirred for 25 minutes to obtain a mixture A.

[0080] Add steareth-20 to the mixture A, raise the temperature to 70° C., and homogenize at 1500 r / min for 5 minutes. Then, place the mixture in 3% by mass ammonium sulfate for salting out for 28 hours at a temperature of 5° C., and dialyze with purified water to obtain a mixture B.

[0081] 1,2-Hexanediol and ethylhexylglycerin were added to the mixture B, and the mixture was homogenized at a speed of 1200 r / min for 3 minutes, and then allowed to stand for degassing for 50 minutes to obtain an anti-aging and anti-wrinkle composition containing multiple polypeptides.

[0082] Comparative Examples 1 to 15:

[0083] Compared with Example 3, the ingredients and component ratios in Comparative Examples 1 to 15 are different, as shown in Table 1.

[0084] 1. The anti-aging and anti-wrinkle compositions containing multiple polypeptides in Examples 1 to 3 and the anti-aging and anti-wrinkle compositions in Comparative Examples 1 to 15 were tested for elastase inhibition rate, DPPH free radical scavenging rate, and ROS scavenging rate. The results are shown in Table 1.

[0085] Table 1:

[0086]

[0087]

[0088]

[0089]

[0090] From the data analysis in Table 1, it can be seen that the present invention, by compounding a variety of polypeptide ingredients and forming a synergistic enhancement system of polypeptides, takes acetyl hexapeptide-8 and snake venom peptide as the core to establish a dual inhibition pathway for dynamic lines; compound hexapeptide-9 and palmitoyl pentapeptide-4 to repair the matrix and promote the regeneration of dermal structural proteins; supplemented with μ-cono peptide, palmitoyl tripeptide-1 and palmitoyl tripeptide-5, strengthen the improvement of static lines and increase skin density; finally supplemented with acetyl tetrapeptide-9 to activate elastin and optimize contour support, achieving the synergistic triple effects of anti-wrinkle, firming and repair, with a better inhibition rate of elastase, excellent free radical scavenging ability, and achieving significant antioxidant effect, excellent anti-aging and anti-wrinkle effect.

[0091] In order to further verify the effects of the anti-aging and anti-wrinkle composition containing multiple polypeptides of the present invention, the following transdermal tests and application tests were conducted.

[0092] 2. Transdermal test

[0093] Rats were killed by cervical dislocation, and the abdominal skin was removed to remove subcutaneous fat. The skin was rinsed with normal saline and stored in normal saline at 4°C until use. Before use, the skin was soaked in pH 7.4 PBS solution for 30 min, rinsed, and the surface moisture was dried with filter paper.

[0094] Test samples: Anti-aging and anti-wrinkle composition samples containing multiple polypeptides obtained in Examples 1 to 3;

[0095] Test method: Franz drug transdermal diffusion tester was used for testing, with a receiving cell volume of 6.5 mL and a transdermal diffusion area of ​​3 cm 2 PBS (pH 7.0) was used as a diffusion grafting agent. The corresponding sample was evenly coated on the surface of the ex vivo skin. The test was carried out at 200 rpm and 35 ± 0.5 ° C. 0.5 mL of sample was taken at 4 h, 8 h, 12 h, and 24 h (while adding an equal amount of isothermal PBS). The absorbance per unit area (cm 2 ) Transmission amount Qm, the test results are shown in Table 2.

[0096] Table 2: Transdermal test results

[0097]

[0098] From the data analysis in Table 2, it can be seen that the anti-aging and anti-wrinkle composition containing multiple polypeptides of the present invention can easily penetrate into the internal cells of the skin, enhance the skin's immune repair activity, improve the skin's texture and anti-wrinkle effect, and can be absorbed and effectively utilized by the skin, thereby exerting its anti-aging and anti-wrinkle efficacy.

[0099] 3. Application testing:

[0100] Test samples: Examples 1 to 3 obtained anti-aging and anti-wrinkle composition samples containing multiple polypeptides.

[0101] Testing method: Volunteers with sagging skin and wrinkles (including fine lines and dry lines) were screened and tested with the samples in each embodiment; the volunteers applied the product once in the morning and once in the evening after cleansing their faces. No other functional products were used at the same time. After a 30-day trial, the volunteers evaluated the application experience, skin firming effect, skin smoothness after use, and dry lines and fine lines on the face. The percentage of people corresponding to each evaluation interval relative to the total number of people in the group was calculated; each group had 50 people. The statistical results are shown in Table 3 (data are rounded off).

[0102] Table 3: Application test results

[0103]

[0104] From the data analysis in Table 3, it can be seen that the anti-aging and anti-wrinkle composition containing multiple polypeptides of the present invention has the effect of significantly reducing fine lines in actual use, and can improve skin pores and skin texture, making the skin smoother and more delicate, and can also exert its significant skin firming and lifting effects, thereby achieving the purpose of anti-aging and anti-wrinkle.

[0105] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0106] The above-described embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the patent for this invention shall be determined by the appended claims.

Claims

1. An anti-aging and anti-wrinkle composition containing multiple polypeptides, characterized in that: The anti-aging and anti-wrinkle composition comprises the following components in percentage by mass: Acetyl hexapeptide-8: 0.05-0.2%, snake venom peptide: 0.01-0.1%, hexapeptide-9: 0.01-0.2%, arginine polypeptide: 0.001-0.1%, μ-cono peptide: 0.01-0.1%, acetyl tetrapeptide-9: 0.01-0.1%, palmitoyl pentapeptide-4: 0.01-0.1%, palmitoyl tripeptide-1: 0.01-0.1%, palmitoyl tripeptide-5: 0.01-0.1%, butylene glycol: 15-40%, glycerin: 5-30%, 1,2-hexanediol: 0.5-5%, ethylhexylglycerin: 0.01-0.1%, steareth-20: 1-5%, purified water: balance 2. The anti-aging and anti-wrinkle composition according to claim 1, characterized in that: The arginine polypeptide may be replaced by a lysine polypeptide.

3. The anti-aging and anti-wrinkle composition according to claim 2, characterized in that: The anti-aging and anti-wrinkle composition comprises the following components in percentage by mass: Acetyl hexapeptide-8: 0.10%, snake venom peptide: 0.05%, hexapeptide-9: 0.05%, Arginine Polypeptide / Lysine Polypeptide: 0.005%, μ-Conopeptide: 0.05%, Acetyl Tetrapeptide-9: 0.012%, Palmitoyl Pentapeptide-4: 0.012%, Palmitoyl Tripeptide-1: 0.012%, Palmitoyl Tripeptide-5: 0.012%, Butylene Glycol: 30%, Glycerin: 20%, 1,2-Hexanediol: 2.0%, Ethylhexylglycerin: 0.05%, Stearyl Alcohol Polyether-20: 2.0%, Purified Water: 45.697%.

4. The anti-aging and anti-wrinkle composition according to claim 2, characterized in that: The anti-aging and anti-wrinkle composition comprises the following components in percentage by mass: Acetyl Hexapeptide-8: 0.05%, Snake Venom-like Peptide: 0.10%, Hexapeptide-9: 0.10%, Arginine / Lysine Peptide: 0.005%, μ-Conopeptide: 0.02%, Acetyl Tetrapeptide-9: 0.02%, Palmitoyl Pentapeptide-4: 0.02%, Palmitoyl Tripeptide-1: 0.02%, Palmitoyl Tripeptide-5: 0.02%, Butylene Glycol: 20%, Glycerin: 30%, 1,2-Hexanediol: 1.0%, Ethylhexylglycerin: 0.15%, Steareth-20: 2.0%, Purified Water: Balance 5. The anti-aging and anti-wrinkle composition according to claim 2, characterized in that: The anti-aging and anti-wrinkle composition comprises the following components in percentage by mass: Acetyl Hexapeptide-8: 0.2%, Snake Venom-like Peptide: 0.10%, Hexapeptide-9: 0.05%, Arginine / Lysine Peptide: 0.01%, μ-Conopeptide: 0.05%, Acetyl Tetrapeptide-9: 0.012%, Palmitoyl Pentapeptide-4: 0.012%, Palmitoyl Tripeptide-1: 0.012%, Palmitoyl Tripeptide-5: 0.012%, Butylene Glycol: 40%, Glycerin: 20%, 1,2-Hexanediol: 2.0%, Ethylhexylglycerin: 0.05%, Steareth-20: 2.0%, Purified Water: Balance 6. A method for preparing an anti-aging and anti-wrinkle composition containing multiple polypeptides, characterized in that: The preparation method is used to prepare the anti-aging and anti-wrinkle composition according to any one of claims 1 to 5, and the preparation method comprises the following steps: The purified water was heated to 40° C. to 45° C., and then butanediol and glycerol were added, and the mixture was stirred at a speed of 300 r / min to 500 r / min for 10 minutes. Acetyl hexapeptide-8, snake venom peptide, hexapeptide-9, arginine peptide / lysine peptide, μ-conopeptide, acetyl tetrapeptide-9, palmitoyl pentapeptide-4, palmitoyl tripeptide-1, and palmitoyl tripeptide-5 were added in sequence, and the mixture was stirred for 15 minutes to 30 minutes to obtain a mixture A. Add steareth-20 to the mixture A, raise the temperature to 60° C. to 75° C., and homogenize at a speed of 1000 r / min to 2000 r / min for 3 min to 5 min to obtain a mixture B; 1,2-Hexanediol and ethylhexylglycerin are added to the mixture B, and the mixture is homogenized at a speed of 1000 r / min to 1500 r / min for 1 to 5 minutes, and then allowed to stand for degassing to obtain an anti-aging and anti-wrinkle composition.

7. An application of an anti-aging and anti-wrinkle composition containing multiple polypeptides, characterized in that: The application is the application of the anti-aging and anti-wrinkle composition obtained by the preparation method according to claim 6 in the preparation of anti-aging and anti-wrinkle skin care products.

8. The use according to claim 7, characterized in that The anti-aging and anti-wrinkle skin care product comprises the anti-aging and anti-wrinkle composition according to any one of claims 1 to 5 and chemically acceptable auxiliary materials.

9. The use according to claim 8, characterized in that The auxiliary material is at least one of acetate, hydrochloride, citrate and trifluoroacetate.

10. The use according to claim 7, characterized in that The dosage form of the anti-aging and anti-wrinkle skin care product is at least one of an aqueous solution, an emulsion, a gel, a cream, a powder, an oil, a spray and a mask.

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